Abstract:
:ISG20 is an interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease superfamily. We have solved the crystal structure of human ISG20 complexed with two Mn2+ ions and uridine 5'-monophosphate (UMP) at 1.9 A resolution. Its structure, including that of the active site, is very similar to those of the corresponding domains of two DEDDh-group DNases, the epsilon subunit of Escherichia coli DNA polymerase III and E. coli exonuclease I, strongly suggesting that its catalytic mechanism is identical to that of the two DNases. However, ISG20 also has distinctive residues, Met14 and Arg53, to accommodate hydrogen bonds with the 2'-OH group of the UMP ribose, and these residues may be responsible for the preference of ISG20 for RNA substrates.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Horio T,Murai M,Inoue T,Hamasaki T,Tanaka T,Ohgi Tdoi
10.1016/j.febslet.2004.09.074keywords:
subject
Has Abstractpub_date
2004-11-05 00:00:00pages
111-6issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579304012062journal_volume
577pub_type
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