Abstract:
:Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from the ischemic zone. In postischemic tissue, NF-kappaB levels increased biphasically with peak levels at 15 min and again at 3 h R. Immunoblotting showed minimal NF-kappaB p50 subunit at all times, with changes in p65 similar to EMSA results. Northern blots showed low p50 and increased p65 expression levels at both 2 and 3 h R. By contrast, AP-1 increased monophasically, with peak levels at 15 min R, which dropped steadily thereafter. These results indicate that NF-kappaB and AP-1 are differentially regulated during reperfusion, which may be a control mechanism for gene expression in reperfused myocardium.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chandrasekar B,Freeman GLdoi
10.1016/s0014-5793(96)01426-3subject
Has Abstractpub_date
1997-01-13 00:00:00pages
30-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(96)01426-3journal_volume
401pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzym...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.007
更新日期:2004-11-05 00:00:00
abstract::Retroviruses have been etiologically implicated with leukemia in humans and animals. Understanding the virus life cycle, the proteins and enzymes involved in its replication, is essential for developing potent anti-viral drugs. Phosphorylation of retroviral proteins may alter their shape in such a way as to increase o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00820-4
更新日期:1996-09-02 00:00:00
abstract::A functional rat heart Na(+)-Ca2+ exchanger gene has been obtained by splicing and ligating two partially overlapping clones isolated from a rat heart lambda ZAP cDNA library. The deduced primary structure of the protein encoded by the open reading frame corresponds to 971 amino acids, that can be organized into 12 tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81737-k
更新日期:1993-01-18 00:00:00
abstract::Tobacco SABP2, a 29kDa protein catalyzes the conversion of methyl salicylic acid (MeSA) into salicylic acid (SA) to induce SAR. Pretreatment of plants with acibenzolar-S-methyl (ASM), a functional analog of salicylic acid induces systemic acquired resistance (SAR). Data presented in this paper suggest that SABP2 catal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.046
更新日期:2010-08-04 00:00:00
abstract::Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13651
更新日期:2020-02-01 00:00:00
abstract::We previously obtained evidence for intrinsic aminopeptidase activity for leukotriene (LT)A4 hydrolase, an enzyme characterized to specifically catalyse the hydrolysis of LTA4 to LTB4, a chemotactic compound. From a sequence homology search between LTA4 hydrolase and several aminopeptidases, it became clear that they ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80806-r
更新日期:1992-09-14 00:00:00
abstract::Rho GTPases participate in various important signaling pathways and have been implicated in myogenic differentiation. Here the first evidence is provided that in C2C12 myoblasts sphingosine 1-phosphate (SPP) rapidly and transiently induced membrane association of Rho A in a pertussis toxin-insensitive manner. The bioa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02039-1
更新日期:2000-09-29 00:00:00
abstract::In photosynthetic reaction centers, the excitation with light leads to the formation of a charge separated state across the photosynthetic membrane. For the reaction center of purple non-sulphur bacteria, it was previously generally assumed that this primary charge separation could only start with the excitation of th...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(99)00810-8
更新日期:1999-07-16 00:00:00
abstract::We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and alpha1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00527-1
更新日期:2003-06-19 00:00:00
abstract::Several blood-feeding organisms, including the malaria parasite detoxify haem released from host haemoglobin by conversion to the insoluble crystalline ferriprotoporphyrin IX dimer known as haemozoin. To date the mechanism of haemozoin formation has remained unknown, although lipids or proteins have been suggested to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.043
更新日期:2006-09-18 00:00:00
abstract::Linkage between the high-capacity Ca2+-binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca2+-release during skeletal muscle excitation-contraction coupling. However, no direct biochemical evidence exists showing a connection between these high-molecular-mass complexes in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00621-8
更新日期:1998-06-16 00:00:00
abstract::A variety of mutations leading to amino acid substitutions have been described in the transthyretin gene in association with different familial amyloidoses and have been implicated to be involved in the pathogenesis of amyloid deposits. However, there has been disagreement whether or not a transthyretin mutation is pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80387-i
更新日期:1991-04-09 00:00:00
abstract::Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00335-9
更新日期:1997-05-19 00:00:00
abstract::Piriprost and nordihydroguiaretic acid (NDGA), specific inhibitors of arachidonate lipoxygenase, inhibited phytohaemagglutinin (PHA)-stimulated breakdown of inositol lipids in human T lymphocytes. The dual inhibitors eicosatetraynoic acid (ETYA) and BW 755C, which inhibit both lipoxygenase and cyclooxygenase, also had...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81621-7
更新日期:1989-11-20 00:00:00
abstract::Terminal oligopyrimidine (TOP) mRNAs are a group of messengers translationally regulated according to the growth status of the cell. Two hypotheses have been proposed for the mechanism of the regulation: (i) there is a specific translational regulator which can reversibly alter TOP-mRNA structure, (ii) a component of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01209-x
更新日期:1997-10-27 00:00:00
abstract::The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80847-0
更新日期:1988-04-25 00:00:00
abstract::Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.040
更新日期:2009-04-02 00:00:00
abstract::Lysosomal/phagosomal membranes of mammalian cells are coated by highly conserved glycoproteins (lamps) that are thought to protect the membranes from degradation. Interestingly, we identified an amino acid sequence in human lamp-1 characteristic of a cleavage site for the Neisseria gonorrhoeae IgA1 protease. Furthermo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00163-4
更新日期:1997-03-17 00:00:00
abstract::The insulin receptor family consists of the homologous tyrosine kinase receptors, insulin receptor (IR), insulin-like growth factor 1 receptor (IGF1R) and insulin receptor-related receptor. The three-dimensional structures of the tyrosine kinase domain of the IR and the first three extracellular domains (L1, Cys-rich ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01509-9
更新日期:1998-12-18 00:00:00
abstract::Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.04.055
更新日期:2005-06-06 00:00:00
abstract::The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Stre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.07.010
更新日期:2014-08-25 00:00:00
abstract::The binding of Mg2+ ADP to both rabbit skeletal and bovine cardiac myofibrils has been studied at two different temperatures. In each case a single class of binding sites was observed with a binding constant very close to that reported for the analogous actomyosin-subfragment one but much weaker than that seen with th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81067-4
更新日期:1984-08-20 00:00:00
abstract::The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80040-0
更新日期:1986-01-01 00:00:00
abstract::Galectin-3 is a galactose-specific lectin which has been shown to be involved in several biological functions such as cell growth regulation, cell aggregation and cell differentiation. The partial cloning of the human genomic sequences reveals the presence of a 651 bp intron, 18 bp downstream of the translation initia...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00310-6
更新日期:1995-04-17 00:00:00
abstract::Chemotaxis induction is a major effect evoked by stimulation of the chemokine receptor CXCR4 with its sole ligand CXCL12. We now report that treatment of CHP-100 human neuroepithelioma cells with the glucosylceramide synthase (GCS) inhibitor DL-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibits CXCR4...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.003
更新日期:2007-06-12 00:00:00
abstract::Plasminogen activation is regulated by the interaction between urokinase-type plasminogen activator (uPA) and its specific glycolipid-anchored cell surface receptor (uPAR). uPAR is composed of three homologous domains and is the only multi-domain member of the Ly-6 family of glycolipid-anchored membrane proteins. Rece...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(94)00674-1
更新日期:1994-08-01 00:00:00
abstract::We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.12.005
更新日期:2013-01-31 00:00:00
abstract::Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00303-q
更新日期:1995-04-24 00:00:00
abstract::Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81051-7
更新日期:1987-06-29 00:00:00
abstract::The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(96)00631-x
更新日期:1996-07-22 00:00:00