Evidence for phosphorylation of rat liver glucose-regulated protein 58, GRP58/ERp57/ER-60, induced by fasting and leptin.

abstract：:The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e. TOCSY) experiments have been conducted for both the deuterated and natural-abundance samples. Using a 54 ms mixing time correlation peaks can be seen for all four...

journal_title：FEBS letters

authors： LeMaster DM

更新日期：1988-06-20 00:00:00

abstract：:The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with a DNA probe internal to the PCK1 gene was found only in cells ...

journal_title：FEBS letters

authors： Valdés-Hevia MD,de la Guerra R,Gancedo C

更新日期：1989-12-04 00:00:00

abstract：:A structural tree for proteins and domains containing S-like beta-sheets has been constructed. An S-like beta-sheet is taken as a starting structure in modelling or as a root structure of the tree. Larger structures are obtained by a stepwise addition of beta-strands and/or alpha-helices to the root S-like beta-sheet ...

journal_title：FEBS letters

authors： Efimov AV

更新日期：1998-10-23 00:00:00

abstract：:Cytochrome bd is a bacterial respiratory oxidase carrying three hemes but no copper. We show that nitric oxide (NO) reacts with the intermediate F of cytochrome bd from Azotobacter vinelandii: (i) with a 1:1 stoichiometry, (ii) rapidly (k=1.2 +/- 0.1 x 10(5)M(-1)s(-1) at 20 degrees C), and (iii) yielding the oxidized ...

journal_title：FEBS letters

authors： Borisov VB,Forte E,Sarti P,Brunori M,Konstantinov AA,Giuffrè A

更新日期：2006-09-04 00:00:00

abstract：:Oxaloacetate decarboxylase of Klebsiella pneumoniae was shown to contain between 0.6 and 1.0 mol zinc per mol enzyme in different preparations. The decarboxylase activity was completely abolished after 15 min incubation with 1 mM Hg(NO3)2 in phosphate buffer, while the activity decreased only 20% if the incubation was...

journal_title：FEBS letters

authors： Dimroth P,Thomer A

更新日期：1992-03-23 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...

journal_title：FEBS letters

authors： Hamada M,Kuwabara T,Warashina M,Nakayama A,Taira K

更新日期：1999-11-12 00:00:00

abstract：:To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subun...

journal_title：FEBS letters

authors： Shim EK,Moon CS,Lee GY,Ha YJ,Chae SK,Lee JR

更新日期：2004-09-24 00:00:00

abstract：:Microsomal membranes from the slow-growing Morris hepatoma 9618A catalyze, in the presence of t-butyl hydroperoxide, lower rates of lipid peroxidation than rat liver microsomes. The cytochrome P-450 content of hepatoma microsomes is about 40% that of the liver. SKF 525-A, an inhibitor of mixed-function oxidase, produc...

journal_title：FEBS letters

authors： Borrello S,Galeotti T,Palombini G,Minotti G

更新日期：1986-12-15 00:00:00

abstract：:Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis dem...

journal_title：FEBS letters

authors： Zhou L,Chen X,Shao Z,Zhou P,Knight DP,Vollrath F

更新日期：2003-11-20 00:00:00

abstract：:Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...

journal_title：FEBS letters

authors： Sandberg K,Markwick AJ,Trinh DP,Catt KJ

更新日期：1988-12-05 00:00:00

abstract：:Bilayers made of dioleoylphosphatidylcholine (DOPC)/dipalmitoylphosphatidylcholine (DPPC) mixture containing or not cholesterol (Chl) were used to investigate the interaction of a carrier peptide with membranes. Atomic force microscopy revealed that the C-terminal 9-32 fragment of human calcitonin (hCT (9-32)), free o...

journal_title：FEBS letters

authors： Boichot S,Krauss U,Plénat T,Rennert R,Milhiet PE,Beck-Sickinger A,Le Grimellec C

更新日期：2004-07-02 00:00:00

abstract：:Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 ...

journal_title：FEBS letters

authors： Telfer JF,Green CD

更新日期：1993-08-30 00:00:00

abstract：:Comparative allelotyping of the short arm of human chromosome 3 (3p) in four types of epithelial carcinomas was performed using an identical set of polymorphic markers. In total, 117 samples of non-papillary renal cell carcinoma (RCC), non-small cell lung carcinoma (NSCLC), carcinoma of uterine cervix (CC), and breast...

journal_title：FEBS letters

authors： Braga E,Pugacheva E,Bazov I,Ermilova V,Kazubskaya T,Mazurenko N,Kisseljov F,Liu J,Garkavtseva R,Zabarovsky E,Kisselev L

更新日期：1999-07-09 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

journal_title：FEBS letters

authors： Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

更新日期：1995-04-24 00:00:00

abstract：:Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

journal_title：FEBS letters

authors： Biggar KK,Storey KB

更新日期：2015-11-30 00:00:00

abstract：:The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

journal_title：FEBS letters

authors： Höcker B,Schmidt S,Sterner R

更新日期：2002-01-16 00:00:00

abstract：:The possible molecular heterogeneity of human transferrin receptors was analyzed using two murine monoclonal antibodies, Tü15 and Tü67. Both reagents precipitated from lysates of 125I-labeled HL-60 cells a major component of 88 kDa which could be identified as the transferrin receptor by comparison with the proteins d...

journal_title：FEBS letters

authors： Uchańska-Ziegler B,Wernet P,Liangru S,Ziegler A

更新日期：1984-10-01 00:00:00

abstract：:Blood flukes of the genus Schistosoma currently infect millions of people in tropical and subtropical countries. An enzyme playing a major role in hemoglobin (Hb) degradation by Schistosoma mansoni has been cloned and shown to be highly similar to the human cathepsin D aspartyl proteinase, although presenting a distin...

journal_title：FEBS letters

authors： Silva FP Jr,Ribeiro F,Katz N,Giovanni-De-Simone S

更新日期：2002-03-13 00:00:00

abstract：:Cytoplasmic dynein, a microtubule-based motor protein, is responsible for many cellular functions ranging from cargo transport to cell division. The various functions are carried out by a single isoform of cytoplasmic dynein, thus requiring different forms of motor regulation. A possible pathway to regulate motor func...

journal_title：FEBS letters

authors： Behrens VA,Walter WJ,Peters C,Wang T,Brenner B,Geeves MA,Scholz T,Steffen W

更新日期：2019-02-01 00:00:00

abstract：:Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...

journal_title：FEBS letters

authors： Dehio M,Gómez-Duarte OG,Dehio C,Meyer TF

更新日期：1998-03-06 00:00:00

abstract：:We identified cDNAs coding for homologues to tetrapod prion proteins (PrPs) in Atlantic salmon (Salmo salar) and Japanese pufferfish (Fugu rubripes), which were termed 'similar to PrPs' (stPrPs). Besides significant sequence homologies the fish stPrPs display characteristic structural features in common with tetrapod ...

journal_title：FEBS letters

authors： Oidtmann B,Simon D,Holtkamp N,Hoffmann R,Baier M

更新日期：2003-03-13 00:00:00

abstract：:The homeoprotein Engrailed (Engrailed-1/Engrailed-2, collectively En1/2) is not only a survival factor for mesencephalic dopaminergic (mDA) neurons during development, but continues to exert neuroprotective and physiological functions in adult mDA neurons. Loss of one En1 allele in the mouse leads to progressive demis...

journal_title：FEBS letters

authors： Rekaik H,Blaudin de Thé FX,Prochiantz A,Fuchs J,Joshi RL

更新日期：2015-12-21 00:00:00

abstract：:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...

journal_title：FEBS letters

authors： Will A,Tanner W

更新日期：1996-02-26 00:00:00

abstract：:The CA microsatellite repeat marker, D7S522, is located at the center of a approximately 1000 kb smallest common deleted region that is lost in many forms of human cancer. It has been proposed that a putative tumor suppressor gene lies in close proximity to D7S522, within this smallest common deleted region. However, ...

journal_title：FEBS letters

authors： Engelman JA,Zhang XL,Lisanti MP

更新日期：1999-04-09 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this rea...

journal_title：FEBS letters

authors： Gargano N,Cattaneo A

更新日期：1997-09-15 00:00:00

abstract：:The Mohr-Tranebjaerg syndrome (MTS), a neurodegenerative syndrome characterized by progressive sensorineural hearing loss, dystonia, mental retardation and blindness, is a mitochondrial disease caused by mutations in the deafness/dystonia peptide 1 (DDP1) gene. DDP1 shows similarity to the yeast proteins Tim9, Tim10 a...

journal_title：FEBS letters

authors： Bauer MF,Rothbauer U,Mühlenbein N,Smith RJ,Gerbitz K,Neupert W,Brunner M,Hofmann S

更新日期：1999-12-24 00:00:00

abstract：:The protein kinase D family of enzymes consists of three isoforms: PKD1/PKCmu PKD2 and PKD3/PKCnu. They all share a similar architecture with regulatory sub-domains that play specific roles in the activation, translocation and function of the enzymes. The PKD enzymes have recently been implicated in very diverse cellu...

journal_title：FEBS letters

authors： Rykx A,De Kimpe L,Mikhalap S,Vantus T,Seufferlein T,Vandenheede JR,Van Lint J

更新日期：2003-07-03 00:00:00

abstract：:The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality ...

journal_title：FEBS letters

authors： Skottman H,Dilber MS,Hovatta O

更新日期：2006-05-22 00:00:00