The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom.

abstract：:The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...

journal_title：FEBS letters

authors： Müller C,Lukas P,Böhmert M,Hildebrandt JP

更新日期：2020-03-01 00:00:00

abstract：:The binding of phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)) to profilin at a region distinct from the actin interaction surface is demonstrated by experiments with covalently cross-linked profilin:beta-actin. The result is in agreement with observations made with several mutant profilins and provides strong ev...

journal_title：FEBS letters

authors： Skare P,Karlsson R

更新日期：2002-07-03 00:00:00

abstract：:The ABCG2 protein has a key role in the transport of a wide range of structurally dissimilar endo- and xenobiotics in the human body, especially in the tissue barriers and the metabolizing or secreting organs. The human ABCG2 gene harbors a high number of polymorphisms and mutations, which may significantly modulate i...

journal_title：FEBS letters

authors： Sarkadi B,Homolya L,Hegedűs T

更新日期：2020-10-04 00:00:00

abstract：:Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

journal_title：FEBS letters

authors： Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

更新日期：2018-04-01 00:00:00

abstract：:Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...

journal_title：FEBS letters

authors： Abe M,Hashimoto H,Yoda K

更新日期：1999-09-24 00:00:00

abstract：:N-glycosidically-linked glycans released by hydrazinolysis of human factor VIII/von Willebrand factor (FVIII/vWf) were separated by high-voltage electrophoresis. Five fractions were obtained, one of them representing 60% of the total amount of the N-glycosidically-linked glycans of FVIII/vWf. On the basis of the carbo...

journal_title：FEBS letters

authors： Debeire P,Montreuil J,Samor B,Mazurier C,Goudemand M,van Halbeek H,Vliegenthart JF

更新日期：1983-01-10 00:00:00

abstract：:Phenylalanine ammonia-lyase immobilized within semipermeable microcapsules has an assayed enzyme activity which is 20% +/- 4% of the enzyme in free solution. The Km for the immobilized enzyme remained the same as that of the free enzyme. The pH optimum also remained unchanged at pH 8.5 +/- 1.0. At the lower pH range, ...

journal_title：FEBS letters

authors： Bourget L,Chang TM

更新日期：1985-01-21 00:00:00

abstract：:The final step in the biosynthesis of nicotinamide-adenine dinucleotide, a major coenzyme in cellular redox reactions and involved in intracellular signaling, is catalyzed by the enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT). The X-ray structure of human NMNAT in complex with nicotinamide mononucleoti...

journal_title：FEBS letters

authors： Werner E,Ziegler M,Lerner F,Schweiger M,Heinemann U

更新日期：2002-04-10 00:00:00

abstract：:A new secondary structure model for parts of the 7 SL RNA is proposed which indicates for a stretch of at least 40 bases a strong structural homology to the ribosomal protein L5 binding site of eukaryotic 5 S rRNA. It is suggested that the 5 S rRNA-like structural part of 7 SL RNA mediates binding of the signal recogn...

journal_title：FEBS letters

authors： Boehm S

更新日期：1987-02-09 00:00:00

abstract：:Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...

journal_title：FEBS letters

authors： Takahashi M,Tezuka T,Kakegawa H,Katunuma N

更新日期：1994-03-07 00:00:00

abstract：:Nanoscale protein supported phospholipid bilayer discs, or Nanodiscs, were produced for the purpose of studying the phase transition behavior of the incorporated lipids. Nanodiscs and vesicles were prepared with two phospholipids, dipalmitoyl phosphatidylcholine and dimyristoyl phosphatidylcholine, and the phase trans...

journal_title：FEBS letters

authors： Shaw AW,McLean MA,Sligar SG

更新日期：2004-01-02 00:00:00

abstract：:Reticulocyte-type 15-lipoxygenase is known to dioxygenate phospholipids without preceding action of phospholipases A2 (PLA2). Therefore we studied the reaction of the secretory PLA2s (sPLA2) from pancreas and snake venom, and of the human cytosolic PLA2 (cPLA2) with 1-palmitoyl-2-arachidonoyl phosphatidylcholine (PAPC...

journal_title：FEBS letters

authors： Chaitidis P,Schewe T,Sutherland M,Kühn H,Nigam S

更新日期：1998-09-04 00:00:00

abstract：:Two clones were isolated by screening a shrimp hepatopancreas cDNA library with a DNA fragment obtained by PCR amplification using two oligonucleotides based on the partial protein sequence of Penaeus vanameii chymotrypsin purified earlier. One of these clones, PVC 7 contains a complete cDNA coding for a serine protea...

journal_title：FEBS letters

authors： Sellos D,Van Wormhoudt A

更新日期：1992-09-14 00:00:00

abstract：:Monomeric and trimeric PS I complexes missing the three stromal subunits E,C and D (termed PS I core complexes) were prepared from the thermophilic cyanobacterium Synechococcus sp. by incubation with urea. The subunits E,C and D are sequentially removed. In the monomeric PS I the subunit C is removed with a half life ...

journal_title：FEBS letters

authors： Lüneberg J,Fromme P,Jekow P,Schlodder E

更新日期：1994-01-31 00:00:00

abstract：:Rat peritoneal mast cells, labelled with [14C]arachidonic acid, released histamine and [14C]arachidonic acid upon the addition of A23187. The release induced by low concentrations of A23187 was suppressed by removal of extracellular Na+ and by addition of the Na+/H+ exchange inhibitor, amiloride. Addition of protein k...

journal_title：FEBS letters

authors： Linnebjerg H,Hansen HS,Jensen B

更新日期：1988-11-21 00:00:00

abstract：:The inhibitory effects of various nitrogen-containing sugars on porcine kidney trehalase were studied. Validamycin A, validoxylamine A and MDL 25,637 were found to be potent, time-dependent inhibitors of the enzyme in vitro. The validoxylamine A-inhibited enzyme showed slow but reversible reactivation over time (t1/2 ...

journal_title：FEBS letters

authors： Salleh HM,Honek JF

更新日期：1990-03-26 00:00:00

abstract：:The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T ...

journal_title：FEBS letters

authors： Mullin M,Lightfoot K,Clarke R,Miller M,Lahesmaa R,Cantrell D

更新日期：2007-09-04 00:00:00

abstract：:Treatment of either crude or purified preparations of the gamma-aminobutyrate (GABA)/benzodiazepine receptor complex with arginine-specific reagents resulted in a time- and concentration-dependent loss of [3H]muscimol binding activity. Following exposure to either 2,3-butanedione or phenylglyoxal (less than or equal t...

journal_title：FEBS letters

authors： Widdows KB,Kirkness EF,Turner AJ

更新日期：1987-09-28 00:00:00

abstract：:Activities of prevalent protein phosphatases decreased by nearly 95% and those of individual protein kinases were differentially reduced at low temperature. Inhibition of phosphatase activity at temperatures below 12 degrees C resulted in marked hyperphosphorylation of a 58-kDa protein (PP58). The temperature threshol...

journal_title：FEBS letters

authors： Monroy AF,Labbé E,Dhindsa RS

更新日期：1997-06-30 00:00:00

abstract：:Human Bfl-1 is an anti-apoptotic Bcl-2 family member. Here, we found that Bfl-1 was converted into a potent death-promoting protein by green fluorescent protein (GFP) fusion with its N-terminus. The transient expression of GFP-Bfl-1 induced cytochrome c release and triggered apoptosis in 293T cells, which depended on ...

journal_title：FEBS letters

authors： Ko JK,Choi KH,Kim HJ,Choi HY,Yeo DJ,Park SO,Yang WS,Kim YN,Kim CW

更新日期：2003-09-11 00:00:00

abstract：:Adaptation to hypoxia is essential for tumor progression. Transcriptional activation of hypoxia-regulated genes is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1alpha and ARNT (Ah receptor nuclear translocator; HIF-1beta). Using representational difference analysis, we identified three novel hy...

journal_title：FEBS letters

authors： Saarikoski ST,Rivera SP,Hankinson O

更新日期：2002-10-23 00:00:00

abstract：:Stomach infection with pathogenic strains of Helicobacter pylori causes in some patients severe gastroduodenal diseases. These bacteria produce various virulence factors and, here, we review the recent acquisition on the biochemical mode of action of three major factors. We discuss the role of urease both as buffer of...

journal_title：FEBS letters

authors： Montecucco C,Papini E,de Bernard M,Zoratti M

更新日期：1999-06-04 00:00:00

abstract：:A recent report of electrical measurements on oriented bacteriorhodopsin in gels [(1986) FEBS Lett. 195, 164 168] concluded that low concentrations of diamines reversed the direction of the proton pump. Calculations are presented which show that in low diamine concentrations, charge displacements of the counterion atm...

journal_title：FEBS letters

authors： Marinetti T

更新日期：1987-05-25 00:00:00

abstract：:Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...

journal_title：FEBS letters

authors： Ohnuma T,Umemoto N,Kondo K,Numata T,Fukamizo T

更新日期：2013-08-19 00:00:00

abstract：:Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...

journal_title：FEBS letters

authors： Shuvaev VV,Siest G

更新日期：1996-03-25 00:00:00

abstract：:The role of p53 as the "guardian of the genome" in differentiated somatic cells, triggering various biological processes, is well established. Recent studies in the stem cell field have highlighted a profound role of p53 in stem cell biology as well. These studies, combined with basic data obtained 20 years ago, provi...

journal_title：FEBS letters

authors： Aloni-Grinstein R,Shetzer Y,Kaufman T,Rotter V

更新日期：2014-08-19 00:00:00

abstract：:The KlICL1 gene, encoding isocitrate lyase in Kluyveromyces lactis, is essential for ethanol utilization. Deletion analyses identified two functional promoter elements, CSRE-A and CSRE-B. Transcription is activated on ethanol, but not on glucose, glycerol or lactate. Expression depends on the KlCat8p transcription fac...

journal_title：FEBS letters

authors： Rodicio R,López ML,Cuadrado S,Cid AF,Redruello B,Moreno F,Heinisch JJ,Hegewald AK,Breunig KD

更新日期：2008-03-05 00:00:00

abstract：:The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequen...

journal_title：FEBS letters

authors： Mohr E,Richter D

更新日期：1990-01-29 00:00:00

abstract：:We present the three-dimensional structure of the N-terminal FK506-binding protein (FKBP)-like domain of the immunophilin FKBP42 from Arabidopsis thaliana. The data provide the structural background for the explanation of key functional properties reported previously. ...

journal_title：FEBS letters

authors： Weiergräber OH,Eckhoff A,Granzin J

更新日期：2006-01-09 00:00:00

abstract：:Cell stimulation raised the cytosolic free Ca2+ concentration, [Ca(2+]i and induces activation of Na+/H+ exchange which raises the cytosolic pH, pHi. Recent studies have addressed the question whether Na+/H+ exchange plays a role in Ca(2+) influx and, specifically, whether a rise in pHi alone suffices to open Ca(2+) c...

journal_title：FEBS letters

authors： Siffert W,Akkerman JW

更新日期：1989-12-18 00:00:00