Evidence for two interaction regions for phosphatidylinositol(4,5)-bisphosphate on mammalian profilin I.

abstract：:Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...

journal_title：FEBS letters

authors： Heldman E,Balan A,Horowitz O,Ben-Zion S,Torten M

更新日期：1985-01-28 00:00:00

abstract：:Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...

journal_title：FEBS letters

authors： Kairane C,Zilmer M,Mutt V,Sillard R

更新日期：1994-05-23 00:00:00

abstract：:The scorpion toxin peptide BeKm-1 was synthesised by fluorenylmethoxycarbonyl solid phase chemistry and folded by air oxidation. The peptide's effects on heterologous human ether-a-go-go-related gene potassium current (I(HERG)) in HEK293 cells were assessed using 'whole-cell' patch clamp. Blockade of I(HERG) by BeKm-1...

journal_title：FEBS letters

authors： Milnes JT,Dempsey CE,Ridley JM,Crociani O,Arcangeli A,Hancox JC,Witchel HJ

更新日期：2003-07-17 00:00:00

abstract：:We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825-2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans. The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely...

journal_title：FEBS letters

authors： Raitio M,Pispa JM,Metso T,Saraste M

更新日期：1990-02-26 00:00:00

abstract：:We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B(12) activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear ma...

journal_title：FEBS letters

authors： Santos F,Vera JL,Lamosa P,de Valdez GF,de Vos WM,Santos H,Sesma F,Hugenholtz J

更新日期：2007-10-16 00:00:00

abstract：:Mitogen-activated protein (MAP) kinases mediate cellular responses to a wide variety of stimuli. Activation of a MAP kinase occurs after phosphorylation by an upstream dual-specificity protein kinase, known as a MAP kinase kinase or MEK. The Arabidopsis thaliana genome encodes 10 MEKs but few of these have been shown ...

journal_title：FEBS letters

authors： Melikant B,Giuliani C,Halbmayer-Watzina S,Limmongkon A,Heberle-Bors E,Wilson C

更新日期：2004-10-08 00:00:00

abstract：:For more than three decades, RNA recombination remained a puzzle and has only begun to be solved in the last few years. The available data provide evidence for a variety of RNA recombination mechanisms. Non-homologous recombination seems to be the most common for RNA. Recent experiments in both the in vitro and the in...

journal_title：FEBS letters

authors： Chetverin AB

更新日期：1999-10-22 00:00:00

abstract：:Neurons grow during development and extend long axons to make contact with their targets with the help of an intrinsic program of axonal growth as well as a range of extrinsic cues and a permissive milieu. Injury events in adulthood induce some neuron types to revert to a regenerative state in the peripheral nervous s...

journal_title：FEBS letters

authors： Doron-Mandel E,Fainzilber M,Terenzio M

更新日期：2015-06-22 00:00:00

abstract：:The enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi (TcG6PDH) catalyses the first step of the pentose phosphate pathway (PPP) and is considered a promising target for the discovery of a new drug against Chagas diseases. In the present work, we describe the crystal structure of TcG6PDH obtained in a ter...

journal_title：FEBS letters

authors： Mercaldi GF,Dawson A,Hunter WN,Cordeiro AT

更新日期：2016-08-01 00:00:00

abstract：:We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and be...

journal_title：FEBS letters

authors： Sakaguchi M,Honda S,Ozawa M,Nishimura O

更新日期：1988-03-28 00:00:00

abstract：:Pro-apoptotic functions of the BH3-only protein BAD are negatively regulated by survival signal-mediated phosphorylation at several serine residues. Recently, we found that the mutant BAD (BADD119G) with an amino acid substitution of Asp (Asp119 to Gly) within the BH3 domain displays strong pro-apoptotic activity in s...

journal_title：FEBS letters

authors： Adachi M,Zhang YB,Imai K

更新日期：2003-09-11 00:00:00

abstract：:alphaArg-376, betaLys-155, and betaArg-182 are catalytically important ATP synthase residues that were proposed to be involved in substrate Pi binding and subsequent steps of ATP synthesis [Senior, A.E., Nadanaciva, S. and Weber, J. (2002) Biochim. Biophys. Acta 1553, 188-211]. Here, it was shown using purified Escher...

journal_title：FEBS letters

authors： Ahmad Z,Senior AE

更新日期：2005-01-17 00:00:00

abstract：:All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr ...

journal_title：FEBS letters

authors： Kato H,Sakai T,Tamura K,Minoguchi S,Shirayoshi Y,Hamada Y,Tsujimoto Y,Honjo T

更新日期：1996-10-21 00:00:00

abstract：:Activation of the signal transducer and activator of transcription 3 (STAT3) in response to interleukin-6 (IL-6) type cytokines involves both phosphorylation of Tyr705, which enables dimerization, nuclear translocation and DNA binding, as well as ser727 phosphorylation. Here, we describe that the 65 C-terminal amino a...

journal_title：FEBS letters

authors： Schuringa JJ,Schepers H,Vellenga E,Kruijer W

更新日期：2001-04-20 00:00:00

abstract：:Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K(+)-efflux and Ca(2+)-influx in Arabidopsis roots. Pre-treatment with either 10μM cAMP or cGMP for 1 or 24h does significantly reduce net K(+)-leakage and Ca(2+)-influx, and in the case of the...

journal_title：FEBS letters

authors： Ordoñez NM,Marondedze C,Thomas L,Pasqualini S,Shabala L,Shabala S,Gehring C

更新日期：2014-03-18 00:00:00

abstract：:SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS). Here we use site-directed mutagenesis to examine the influence of negative charge and conformation at the site on T-ag nuclear import and recognition by the N...

journal_title：FEBS letters

authors： Xiao CY,Jans P,Jans DA

更新日期：1998-12-04 00:00:00

abstract：:Recombinant Plasmodium falciparum glyoxalase I (PfGlx I) was characterized as monomeric Zn(2+)-containing enzyme of 44 kDa. The K(M) value of the methylglyoxal-glutathione adduct is 77+/-15 microM, the k(cat) value being 4000 min(-1) at 25 degrees C and pH 7.0. PfGlx I consists of two halves, each of which is homologo...

journal_title：FEBS letters

authors： Iozef R,Rahlfs S,Chang T,Schirmer H,Becker K

更新日期：2003-11-20 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...

journal_title：FEBS letters

authors： Nambu Y,Ishii-Hyakutake M,Harada K,Mizuno S,Tsuge T

更新日期：2020-02-01 00:00:00

abstract：:Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...

journal_title：FEBS letters

authors： Charnet P,Bourinet E,Dubel SJ,Snutch TP,Nargeot J

更新日期：1994-05-09 00:00:00

abstract：:The amino-terminal part of prion protein (PrP), containing a series of octapeptide repeats with the consensus sequence PHGGGWGQ, has been implicated in the binding of copper ion. This region possesses amino acid residues susceptible to oxidation, such as histidine, lysine, arginine and proline. In this study, we have ...

journal_title：FEBS letters

authors： Shiraishi N,Nishikimi M

更新日期：2002-01-30 00:00:00

abstract：:The effect of neuropeptide Y (NPY) on cytosolic free Ca2+ concentration ([Ca2+]i) was studied in cultured smooth muscle cells from porcine aorta (PASMC) and compared with the effect of bradykinin (BK) and angiotensin II (ATII) on [Ca2+]i. All peptides induced dose-dependent and transient rises in [Ca2+]i which were no...

journal_title：FEBS letters

authors： Mihara S,Shigeri Y,Fujimoto M

更新日期：1989-12-18 00:00:00

abstract：:The induction of adipose conversion in 3T3-L1 cells by bezafibrate has been shown to be enhanced by dibutyryl-cAMP. We here report that the induction of adipose conversion in 3T3-L1 cells, by either bezafibrate and dibutyryl cAMP, or isobutylmethyxanthine alone, is associated with a very early phosphorylation of a 60-...

journal_title：FEBS letters

authors： Brandes R,Arad R,Bar-Tana J

更新日期：1991-07-08 00:00:00

abstract：:Insulin stimulated protein kinase B alpha (PKB alpha) more than 10-fold and decreased glycogen synthase kinase-3 (GSK3) activity by 50 +/- 10% in skeletal muscle and adipocytes. Rapamycin did not prevent the activation of PKB, inhibition of GSK3 or stimulation of glycogen synthase up to 5 min. Thus rapamycin-insensiti...

journal_title：FEBS letters

authors： Cross DA,Watt PW,Shaw M,van der Kaay J,Downes CP,Holder JC,Cohen P

更新日期：1997-04-07 00:00:00

abstract：:Lipids are essential eukaryotic cellular constituents. Lipid metabolism has a strong impact on cell physiology, and despite good progress in this area, many important basic questions remain unanswered concerning the functional diversity of lipid species and on the mechanisms that cells employ to sense and adjust their...

journal_title：FEBS letters

authors： Santos AX,Riezman H

更新日期：2012-08-31 00:00:00

abstract：:Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solub...

journal_title：FEBS letters

authors： Rich DH,Kawai M,Goodman HL,Engelke J,Suttie JW

更新日期：1983-02-07 00:00:00

abstract：:Although insulin secretion is usually regarded as a Ca2+-dependent mechanism, recent studies have suggested the existence of a Ca2+-independent pathway of regulation by glucose. Here, mouse islets were used to compare the contribution of Ca2+-dependent and -independent pathways. Glucose increased insulin release in a ...

journal_title：FEBS letters

authors： Sato Y,Nenquin M,Henquin JC

更新日期：1998-01-09 00:00:00

abstract：:Reconstituted Na+,K+-ATPase from either pig kidney or shark rectal glands was phosphorylated by cAMP dependent protein kinase, PKA. The stoichiometry was approximately 0.9 mol P(i)/mol alpha-subunit in the pig kidney enzyme and approximately 0.2 mol P(i)/mol alpha-subunit in the shark enzyme. In shark, Na+,K+-ATPase P...

journal_title：FEBS letters

authors： Cornelius F,Logvinenko N

更新日期：1996-02-19 00:00:00

abstract：:The interactions between clavulanate and three class C enzymes have been studied in detail. In all cases, the reactions followed branched pathways where 25-150 turnovers occurred before inactivation was completed. Reactivation rates were quite low. The poor efficiency of clavulanate as a class C inactivator appeared t...

journal_title：FEBS letters

authors： Monnaie D,Frere JM

更新日期：1993-11-22 00:00:00

abstract：:Genetically obese (ob/ob) mice develop a marked insulin resistance in brown adipose tissue soon after weaning, and this is paralleled by a fall in the acute activation of the mitochondrial proton conductance pathway in the tissue on cold exposure. Treatment of ob/ob mice with ciglitazone, a new oral hypoglycaemic, led...

journal_title：FEBS letters

authors： Mercer SW,Trayhurn P

更新日期：1986-01-20 00:00:00

abstract：:Post-transcriptional regulation of gene expression by RNA-binding proteins and by small non-coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch-2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RN...

journal_title：FEBS letters

authors： Boudoukha S,Rivera Vargas T,Dang I,Kropp J,Cuvellier S,Gautreau A,Polesskaya A

更新日期：2014-05-02 00:00:00