Abstract:
:N-glycosidically-linked glycans released by hydrazinolysis of human factor VIII/von Willebrand factor (FVIII/vWf) were separated by high-voltage electrophoresis. Five fractions were obtained, one of them representing 60% of the total amount of the N-glycosidically-linked glycans of FVIII/vWf. On the basis of the carbohydrate composition, methylation analysis and 500 MHz 1H-NMR spectroscopy, we describe the primary structure of this major glycan which is of the monosialylated and monofucosylated biantennary N-acetyllactosaminic type.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Debeire P,Montreuil J,Samor B,Mazurier C,Goudemand M,van Halbeek H,Vliegenthart JFdoi
10.1016/0014-5793(83)80334-2subject
Has Abstractpub_date
1983-01-10 00:00:00pages
22-6issue
1eissn
0014-5793issn
1873-3468journal_volume
151pub_type
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