Abstract:
:The reduction and reoxidation kinetics of the first quinone-type electron acceptor in photosystem II, QA-, were measured by fluorescence in a light-tolerant, photosystem I-less strain of the cyanobacterium Synechocystis sp. PCC 6803. In this strain, which shows excellent amplitudes of variable fluorescence, the rate of QA- oxidation after photoreduction of the plastoquinone pool was about half of that in the presence of photosystem I. However, upon addition of 5 mM KCN, QA- decay was very slow, and the rate was comparable to that seen in the presence of diuron, which blocks electron transport between QA and QB. The KCN-imposed block of QA- oxidation was removed efficiently by addition of exogenous quinones that can oxidize the plastoquinone pool. These results indicate that, in the absence of photosystem I, photosystem II-generated electrons are used very effectively by an oxidase located in the thylakoid; this oxidase may be a component of the respiratory chain.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vermaas WF,Shen G,Styring Sdoi
10.1016/0014-5793(94)80638-1subject
Has Abstractpub_date
1994-01-03 00:00:00pages
103-8issue
1eissn
0014-5793issn
1873-3468journal_volume
337pub_type
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