Abstract:
:Elongation factors EF-G and EF-Tu are structural homologues and share near-identical binding sites on the ribosome, which encompass the GTPase-associated centre (GAC) and the sarcin-ricin loop (SRL). The SRL is fixed structure in the ribosome and contacts elongation factors in the vicinity of their GTP-binding site. In contrast, the GAC is mobile and we hypothesize that it interacts with the alpha helix D of the EF-Tu G-domain in the same way as with the alpha helix A of the G'-domain of EF-G. The mutual locations of these helices and GTP-binding sites in the structures of EF-Tu and EF-G are different. Thus, the orientation of the GAC relative to the SRL determines whether EF-G or EF-Tu will bind to the ribosome.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sergiev PV,Bogdanov AA,Dontsova OAdoi
10.1016/j.febslet.2005.09.010keywords:
subject
Has Abstractpub_date
2005-10-24 00:00:00pages
5439-42issue
25eissn
0014-5793issn
1873-3468pii
S0014-5793(05)01103-8journal_volume
579pub_type
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