Inositol phospholipid turnover and protein kinase C translocation are stimulated by poly(I).poly(C) in human amnion cells (UAC).

abstract：:The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...

journal_title：FEBS letters

authors： Forsmark P,Aberg F,Norling B,Nordenbrand K,Dallner G,Ernster L

更新日期：1991-07-08 00:00:00

abstract：:Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To unders...

journal_title：FEBS letters

authors： Fanzani A,Giuliani R,Colombo F,Zizioli D,Presta M,Preti A,Marchesini S

更新日期：2003-07-17 00:00:00

abstract：:Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...

journal_title：FEBS letters

authors： Charnet P,Bourinet E,Dubel SJ,Snutch TP,Nargeot J

更新日期：1994-05-09 00:00:00

abstract：:We transfected the COS-7 cells with cDNAs encoding different human somatostatin receptor (hSSTR) subtypes, and found that hSSTR subtypes mediate not only the inhibition of forskolin-induced cAMP accumulation but also the stimulation of phospholipase C (PLC) and Ca2+ mobilization. Activation of PLC by 1 microM somatost...

journal_title：FEBS letters

authors： Akbar M,Okajima F,Tomura H,Majid MA,Yamada Y,Seino S,Kondo Y

更新日期：1994-07-11 00:00:00

abstract：:Annexins are multifunctional intracellular proteins with Ca2+- and phospholipid-binding properties. Their structures consist of four conserved repeat domains that form the core and a diverse N-terminal tail, from which their functional differences may arise. We searched for cellular proteins that interact with the N-t...

journal_title：FEBS letters

authors： Lee KH,Na DS,Kim JW

更新日期：1999-01-15 00:00:00

abstract：:Protein misfolding and aggregation are linked to several degenerative diseases and are responsible for the formation of bacterial inclusion bodies. Roles of molecular chaperones in promoting protein deposition have been speculated but not proven in vivo. We have investigated the involvement of individual chaperones in...

journal_title：FEBS letters

authors： Carrió MM,Villaverde A

更新日期：2003-02-27 00:00:00

abstract：:We have expressed the cloned rat kappa-opioid receptor in human embryonic kidney 293 cells and studied the ability of kappa-selective ligands to inhibit adenylyl cyclase. In transfected 293 cells, activation of the kappa-opioid receptor by U50,488 and the dynorphins resulted in the inhibition of cAMP accumulation. The...

journal_title：FEBS letters

authors： Lai HW,Minami M,Satoh M,Wong YH

更新日期：1995-02-20 00:00:00

abstract：:We have characterized the cell surface of zebrafish stratified epithelium using a combined approach of light and atomic force microscopy under conditions which simulate wound healing. Microridges rise on average 100 nm above the surface of living epithelial cells, which correlate to bundles of cytochalasin B-insensiti...

journal_title：FEBS letters

authors： Sharma A,Anderson KI,Müller DJ

更新日期：2005-03-28 00:00:00

abstract：:[3H]inositol-labelled products are released from adrenal medullary cells during exocytotic secretion. In 'leaky' cells in which small molecules readily enter and leave the cytoplasm, addition of micromolar calcium ions in the presence of ATP stimulates exocytosis and causes the release of inositol polyphosphates. Thes...

journal_title：FEBS letters

authors： Whitaker M

更新日期：1985-09-09 00:00:00

abstract：:Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of p...

journal_title：FEBS letters

authors： McCarthy L,Bentley-DeSousa A,Denoncourt A,Tseng YC,Gabriel M,Downey M

更新日期：2020-01-01 00:00:00

abstract：:(7-methoxycoumarin-4-yl)Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L- 2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) has been synthesised as a fluorogenic substrate for the matrix metalloproteinases. The highly fluorescent 7-methoxycoumarin group is efficiently quenched by energy transfer t...

journal_title：FEBS letters

authors： Knight CG,Willenbrock F,Murphy G

更新日期：1992-01-27 00:00:00

abstract：:Tissue-transglutaminase (t-TGase) is a family of calcium-dependent enzymes. A Ca2+-independent soluble enzyme, in addition to t-TGase, capable of incorporating polyamines into proteins was demonstrated in rat intestinal mucosa. The Ca2+-independent enzyme was stimulated 2- to 5-fold by Fe2+ and Co2+ ions but inhibited...

journal_title：FEBS letters

authors： Tsai YH,Lai WF,Wu YW,Johnson LR

更新日期：1998-09-18 00:00:00

abstract：:Liposomes can be loaded with weak acids and bases, which exist in solutions in equilibrium with membrane permeable uncharged form, using various gradients across their membranes. Because in some cases the estimated drug concentration in the loaded liposomes exceeds their aqueous solubility we investigated the physical...

journal_title：FEBS letters

authors： Lasic DD,Frederik PM,Stuart MC,Barenholz Y,McIntosh TJ

更新日期：1992-11-09 00:00:00

abstract：:In Gram-positive bacteria the tRNA-dependent T box riboswitch regulates the expression of many amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. The Specifier domain of the T box riboswitch contains the Specifier sequence that is complementary to the tRNA antico...

journal_title：FEBS letters

authors： Chang AT,Nikonowicz EP

更新日期：2013-11-01 00:00:00

abstract：:Galectin-3 is a galactose-specific lectin which has been shown to be involved in several biological functions such as cell growth regulation, cell aggregation and cell differentiation. The partial cloning of the human genomic sequences reveals the presence of a 651 bp intron, 18 bp downstream of the translation initia...

journal_title：FEBS letters

authors： Raimond J,Rouleux F,Monsigny M,Legrand A

更新日期：1995-04-17 00:00:00

abstract：:It is known that a high incidence of hepatocellular carcinoma in rat liver can be induced by such azo dye carcinogens as 3'-methyl-4-dimethylaminoazobenzene (3'-MeDAB). Mammalian S-adenosylmethionine (AdoMet) synthetase exists as two isozymes, non-hepatic-type and liver-type enzymes, which are the products of two diff...

journal_title：FEBS letters

authors： Horikawa S,Kobayashi Y,Sugiyama T,Terashima H,Wada K,Tsukada K,Hirokawa S corrected to Horikawa S

更新日期：1993-11-08 00:00:00

abstract：:Sterically hindered cyclic amines react with hydroxyl radicals in the presence of oxygen to yield stable nitroxide radicals which can be detected by EPR. This reaction provides a nonconventional spin-trapping tool for detection of hydroxyl radicals. ...

journal_title：FEBS letters

authors： Rosenthal I,Krishna CM,Yang GC,Kondo T,Riesz P

更新日期：1987-09-28 00:00:00

abstract：:The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...

journal_title：FEBS letters

authors： Nguyen TN,You DJ,Kanaya E,Koga Y,Kanaya S

更新日期：2013-05-02 00:00:00

abstract：:The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...

journal_title：FEBS letters

authors： Kim HM,Oh GT,Hong DH,Kim MS,Kang JS,Park SM,Han SB

更新日期：1997-07-21 00:00:00

abstract：:PTPRO is often silenced by DNA hypermethylation in primary human tumors and cancer cell lines and functions as a tumor suppressor. Here we show that PTPRO is a target of E2F1. In addition, the microRNA cluster miR-17-92, another target of E2F1, participates in PTPRO regulation. PTPRO mRNA was up-regulated during S pha...

journal_title：FEBS letters

authors： Xu X,Hong Y,Kong C,Xu L,Tan J,Liang Q,Huang B,Lu J

更新日期：2008-08-20 00:00:00

abstract：:Targeting of preproteins to mitochondria and their translocation across the outer membrane are mediated by the mitochondrial receptor complex. This protein complex contains the import receptors MOM19 and MOM72 and the general insertion pore GIP. All seven components of the receptor complex are synthesized in the cytos...

journal_title：FEBS letters

authors： Keil P,Pfanner N

更新日期：1993-04-26 00:00:00

abstract：:In PC12 cells, cAMP stimulates the MAP kinase pathway by an unknown mechanism. Firstly, we examined the role of calcium ion mobilization and of protein kinase C in cAMP-stimulated MAP kinase activation. We show that cAMP stimulates p44mapk independently of these events. Secondly, we studied the role of B-Raf in this p...

journal_title：FEBS letters

authors： Peraldi P,Frödin M,Barnier JV,Calleja V,Scimeca JC,Filloux C,Calothy G,Van Obberghen E

更新日期：1995-01-09 00:00:00

abstract：:Using the polymerase chain reaction (PCR), we identified a gene family including more than 60 members which encoded similar G protein-coupled seven-transmembrane receptors. Sequence analyses of six representatives out of the 60 PCR clones showed that they had significant structural similarity to olfactory and optic re...

journal_title：FEBS letters

authors： Abe K,Kusakabe Y,Tanemura K,Emori Y,Arai S

更新日期：1993-02-01 00:00:00

abstract：:Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known o...

journal_title：FEBS letters

authors： Faoro C,Ataide SF

更新日期：2014-10-16 00:00:00

abstract：:Pro-apoptotic functions of the BH3-only protein BAD are negatively regulated by survival signal-mediated phosphorylation at several serine residues. Recently, we found that the mutant BAD (BADD119G) with an amino acid substitution of Asp (Asp119 to Gly) within the BH3 domain displays strong pro-apoptotic activity in s...

journal_title：FEBS letters

authors： Adachi M,Zhang YB,Imai K

更新日期：2003-09-11 00:00:00

abstract：:Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1...

journal_title：FEBS letters

authors： Fitzpatrick KL,Tyerman SD,Kaiser BN

更新日期：2008-04-30 00:00:00

abstract：:To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...

journal_title：FEBS letters

authors： Hagiwara Y,Nureki O,Tateno M

更新日期：2009-02-18 00:00:00

abstract：:SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in ...

journal_title：FEBS letters

authors： Law PY,Liu YM,Geng H,Kwan KH,Waye MM,Ho YY

更新日期：2006-06-26 00:00:00

abstract：:Partially purified 2-methyleneglutarate mutase from Clostridium barkeri was separated from 3-methylitaconate delta-isomerase by treatment with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) followed by FPLC on the anion exchange column Mono Q in the presence of the detergent. When purified in the da...

journal_title：FEBS letters

authors： Michel C,Buckel W

更新日期：1991-04-09 00:00:00

abstract：:The mitochondrial dynamin-like GTPase Mgm1 exists as a long (l-Mgm1) and a short isoform (s-Mgm1). They both are essential for mitochondrial fusion. Here we show that the isoforms interact in a homotypic and heterotypic manner. Their submitochondrial distribution between inner boundary membrane and cristae was markedl...

journal_title：FEBS letters

authors： Zick M,Duvezin-Caubet S,Schäfer A,Vogel F,Neupert W,Reichert AS

更新日期：2009-07-07 00:00:00