Calcium currents recorded from a neuronal alpha 1C L-type calcium channel in Xenopus oocytes.

abstract：:Polystyrene microtitre wells are commonly used as supports for the enzyme-linked immunosorbent assay (ELISA) method of biomolecular detection, which is employed in the routine diagnosis of a variety of medical conditions. We have used an atomic force microscope (AFM) to directly monitor specific molecular interactions...

journal_title：FEBS letters

authors： Allen S,Davies J,Dawkes AC,Davies MC,Edwards JC,Parker MC,Roberts CJ,Sefton J,Tendler SJ,Williams PM

更新日期：1996-07-22 00:00:00

abstract：:In adipogenesis, growth factors play a crucial role. Using serum depleted condition, we studied the causal role of endothelin-1 (ET-1) and epidermal growth factor (EGF), separately or together, in adipocyte differentiation of 3T3-L1 cells. ET-1 stimulation caused an anti-adipogenic response and this effect was potenti...

journal_title：FEBS letters

authors： Bhattacharya I,Ullrich A

更新日期：2006-10-16 00:00:00

abstract：:Chimeric proteins composed of ricin toxin A chain (RTA) and staphylococcal protein A (PA) have been produced in E. coli. Constructs consisting of N-terminal RTA and C-terminal PA (RTA-PA) or N-terminal PA and C-terminal (PA-RTA) were capable of binding to immunoglobulin G (via PA) and of specifically depurinating 28 S...

journal_title：FEBS letters

authors： O'Hare M,Brown AN,Hussain K,Gebhardt A,Watson G,Roberts LM,Vitetta ES,Thorpe PE,Lord JM

更新日期：1990-10-29 00:00:00

abstract：:A macromolecular complex containing survival of motor neurons (SMN), the spinal muscular atrophy protein, and Gemin2-7 interacts with Sm proteins and snRNAs to carry out the assembly of these components into spliceosomal small nuclear ribonucleoproteins (snRNPs). Here we report the characterization of unr-interacting ...

journal_title：FEBS letters

authors： Carissimi C,Baccon J,Straccia M,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L

更新日期：2005-04-25 00:00:00

abstract：:This paper first discusses the conditions in which a set of differential equations should give stable solutions, starting with linear systems assuming that these do not differ greatly in this respect from non-linear systems. Methods of investigating the stability of particular systems are briefly discussed. Most real ...

journal_title：FEBS letters

authors： Cooper GJ

更新日期：1969-03-01 00:00:00

abstract：:The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...

journal_title：FEBS letters

authors： Olds RJ,Lane DA,Boisclair M,Sas G,Bock SC,Thein SL

更新日期：1992-04-06 00:00:00

abstract：:A simple technique for rapid isolation of mitochondrial DNA (mtDNA) from animal cells is described. The method is based on the selective alkaline denaturation procedure of Birnboim and Doly [(1979) Nucleic Acids Res. 7, 1513-1523] and avoids the use of CsCl gradient centrifugation. The yield of mtDNA is comparable to ...

journal_title：FEBS letters

authors： Palva TK,Palva ET

更新日期：1985-11-18 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the clo...

journal_title：FEBS letters

authors： Taneike I,Zhang HM,Wakisaka-Saito N,Yamamoto T

更新日期：2002-07-31 00:00:00

abstract：:Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...

journal_title：FEBS letters

authors： Rentsch D,Laloi M,Rouhara I,Schmelzer E,Delrot S,Frommer WB

更新日期：1995-08-21 00:00:00

abstract：:The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence...

journal_title：FEBS letters

authors： Kunugi S,Yokoyama M,Sakamoto A

更新日期：1995-04-03 00:00:00

abstract：:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modificati...

journal_title：FEBS letters

authors： Schmidt MC,Hanna MM

更新日期：1986-01-06 00:00:00

abstract：:The serine peptidases, thrombocytin and PA-BJ, isolated from the venom of Bothrops atrox and Bothrops jararaca, respectively, induce platelet aggregation and granule secretion without clotting fibrinogen. The specific platelet aggregation activity of each enzyme was about 15 times lower than that of thrombin. This act...

journal_title：FEBS letters

authors： Santos BF,Serrano SM,Kuliopulos A,Niewiarowski S

更新日期：2000-07-21 00:00:00

abstract：:Treatment of yeast phosphoglycerate kinase (PGK) with trypsin results in a fourfold increase in the Vmax of this enzyme, without affecting the Km. This activation is shown to be due to the removal of the C-terminal lysine residue. The C-terminal sequence folds back over the N-terminal domain and contacts the extreme N...

journal_title：FEBS letters

authors： Adams B,Fowler R,Hudson M,Pain RH

更新日期：1996-04-29 00:00:00

abstract：:We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...

journal_title：FEBS letters

authors： Romero LC,Biswal B,Song PS

更新日期：1991-05-06 00:00:00

abstract：:While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither n...

journal_title：FEBS letters

authors： Sim S,Wang P,Beyer BN,Cutrona KJ,Radhakrishnan ML,Elmore DE

更新日期：2017-03-01 00:00:00

abstract：:Cytosolic Ca2+ concentration and membrane potential were monitored in individual cultured endothelial cells mechanically stimulated with a micropipette attached to the stage of a microscope. Both dimpling and poking of endothelial cells resulted in Ca2+i transients (from 63 +/- 12 to 397 +/- 52 nM, characterized by a ...

journal_title：FEBS letters

authors： Goligorsky MS

更新日期：1988-11-21 00:00:00

abstract：:To elucidate hydroxyapatite-protein interaction, mutant human lysozymes in which the surface charge was modified by site-directed mutagenesis were used. Five mutant human lysozymes (K1A, K13A, K33A, R10A, R14A) were expressed in yeast. The chromatographic behavior of these lysozymes was studied with a HPLC hydroxyapat...

journal_title：FEBS letters

authors： Aizawa T,Koganesawa N,Kamakura A,Masaki K,Matsuura A,Nagadome H,Terada Y,Kawano K,Nitta K

更新日期：1998-01-30 00:00:00

abstract：:A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber a...

journal_title：FEBS letters

authors： Peng HL,Chang HY

更新日期：1993-08-23 00:00:00

abstract：:The solution structure of endothelin-1, a newly discovered potent bicyclic peptide vaso-constrictor agent, has been investigated using 1H NMR conformational constraints and distance geometry calculations. The conformation is constrained by two disulphide bridges between Cys1-Cys15 and Cys3-Cys11 but the NMR data and c...

journal_title：FEBS letters

authors： Munro S,Craik D,McConville C,Hall J,Searle M,Bicknell W,Scanlon D,Chandler C

更新日期：1991-01-14 00:00:00

abstract：:The mechanism of the maize pyruvate, phosphate dikinase-catalyzed conversion of adenosine 5'-triphosphate, orthophosphate and pyruvate to adenosine 5'-monophosphate, inorganic pyrophosphate and phosphoenolpyruvate, respectively, was determined by using transient kinetic techniques. The data obtained demonstrate that c...

journal_title：FEBS letters

authors： Carroll LJ,Dunaway-Mariano D,Smith CM,Chollet R

更新日期：1990-11-12 00:00:00

abstract：:A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a startin...

journal_title：FEBS letters

authors： Osborne S,Cecconato E,Griva S,Garetto F,Calogero R,Rosa C,Bonelli F

更新日期：1993-06-21 00:00:00

abstract：:A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cel...

journal_title：FEBS letters

authors： Fournel-Gigleux S,Jackson MR,Wooster R,Burchell B

更新日期：1989-01-30 00:00:00

abstract：:There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...

journal_title：FEBS letters

authors： Megarity CF,Gill JR,Caraher MC,Stratford IJ,Nolan KA,Timson DJ

更新日期：2014-05-02 00:00:00

abstract：:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...

journal_title：FEBS letters

authors： Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RB

更新日期：1999-03-19 00:00:00

abstract：:It is well known that, in rabbits, the allotypes of the b locus carried by the light chains of the IgG molecules cross-react. Nothing similar has been described for the allotypes of the a locus carried by the heavy chains of the IgG molecules. This paper describes a cross-reaction between the rabbit allotypic markers ...

journal_title：FEBS letters

authors： Renneboog-Squilbin C

更新日期：1969-02-01 00:00:00

abstract：:The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...

journal_title：FEBS letters

authors： Rosenblum JS,Nomanbhoy TK,Kozarich JW

更新日期：2013-06-27 00:00:00

abstract：:Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with ...

journal_title：FEBS letters

authors： Liu MC,Liu MY,Payne WJ,Peck HD Jr,Le Gall J,DerVartanian DV

更新日期：1987-06-29 00:00:00

abstract：:Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...

journal_title：FEBS letters

authors： Bartling B,Hilgefort C,Friedrich I,Silber RE,Simm A

更新日期：2003-12-18 00:00:00

abstract：:Amino acid sequence alignment of the amino-terminal of thrombomodulin and pancreatic stone protein with the hepatic asialoglycoprotein receptor shows that these proteins are homologous. From the known disulfide bridge pattern of other proteins belonging to the same family two disulfide bonds can be predicted. The homo...

journal_title：FEBS letters

authors： Petersen TE

更新日期：1988-04-11 00:00:00

abstract：:A novel peptide from Centruroides noxius Hoffmann scorpion venom was isolated and sequenced. The 37 amino acid peptide belongs to the charybdotoxin sub-family (alphaKTx1) and was numbered member 11. alphaKTx1.11 has 75% sequence identity with iberiotoxin and 54% with charybdotoxin. alphaKTx1.11 revealed specificity fo...

journal_title：FEBS letters

authors： Garcia-Valdes J,Zamudio FZ,Toro L,Possani LD

更新日期：2001-09-21 00:00:00