Abstract:
:The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understanding how these proteins function and how to inhibit them for therapeutic benefit. Recent advances in chemical biological tools have enabled functional interrogation of these enzymes to provide a deeper understanding of their physiological roles. In addition, these innovative platforms have paved the way for a new generation of drugs whose properties have been guided by functional profiling.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rosenblum JS,Nomanbhoy TK,Kozarich JWdoi
10.1016/j.febslet.2013.05.008subject
Has Abstractpub_date
2013-06-27 00:00:00pages
1870-7issue
13eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00350-5journal_volume
587pub_type
杂志文章,评审相关文献
FEBS LETTERS文献大全abstract::Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca(2+) signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca(2...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01463-5
更新日期:1999-11-19 00:00:00
abstract::The 3D structure of two unlabeled FK506 analogs, (R)- and (S)-[18-OH]ascomycin, when bound to [U-13C,15N]FKBP were determined by isotope-filtered 2D NMR experiments. The structures for the R and S isomers that bind tightly to FKBP but lack immunosuppressive activity are compared to each other and to the conformation o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81300-b
更新日期:1992-08-24 00:00:00
abstract::We identified a CpG island at the 5' end of murine leukemia inhibitory factor gene (LIF). The CpG island is 0.6 kb long and covers most of the first exon and first intron. The region is non-methylated, its G+C content is 65% and relative frequency of CpG dinucleotide is 0.7. The block of 150 nucleotides, which is 72% ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80058-3
更新日期:1993-03-15 00:00:00
abstract::Characterization of lignified Zinnia elegans hypocotyls by both alkaline nitrobenzene oxidation and thioacidolysis reveals that coniferyl alcohol units are mainly found as part of 4-O-linked end groups and aryl-glycerol-beta-aryl ether (beta-O-4) structures. Z. elegans hypocotyls also contain a basic peroxidase (EC 1....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03339-2
更新日期:2002-10-09 00:00:00
abstract::Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.063
更新日期:2010-01-04 00:00:00
abstract::Plasminogen activation is regulated by the interaction between urokinase-type plasminogen activator (uPA) and its specific glycolipid-anchored cell surface receptor (uPAR). uPAR is composed of three homologous domains and is the only multi-domain member of the Ly-6 family of glycolipid-anchored membrane proteins. Rece...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(94)00674-1
更新日期:1994-08-01 00:00:00
abstract::The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK8 greater than CCK33 greater than desulphated CCK8 greater than CCK4. Binding of CCK to its receptor was abolish...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80343-3
更新日期:1983-01-10 00:00:00
abstract::We have investigated the possible role of selected negatively-charged amino acids of the sodium and chloride-coupled GABA transporter GAT-1 on sodium binding. These residues located adjacent to putative transmembrane domains and which are conserved throughout the large superfamily of neurotransmitter transporters were...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00859-8
更新日期:1995-08-28 00:00:00
abstract::Excessive reactive oxygen species (ROS) play a key role in the pathogenesis of diabetic nephropathy. The thioredoxin (TRX) system, a major thiol antioxidant system, regulates the reduction of intracellular ROS. Here we show that high glucose (HG) inhibits TRX ROS-scavenging function through p38 mitogen-activated prote...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.07.010
更新日期:2010-08-04 00:00:00
abstract::Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.050
更新日期:2006-03-20 00:00:00
abstract::We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined wit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.070
更新日期:2006-07-24 00:00:00
abstract::Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81043-7
更新日期:1985-09-23 00:00:00
abstract::Protein misfolding and aggregation are linked to several degenerative diseases and are responsible for the formation of bacterial inclusion bodies. Roles of molecular chaperones in promoting protein deposition have been speculated but not proven in vivo. We have investigated the involvement of individual chaperones in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00126-1
更新日期:2003-02-27 00:00:00
abstract::Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80932-7
更新日期:1992-11-09 00:00:00
abstract::The genetic modification of lysozyme was attempted to improve the bactericidal activity against Gram-negative bacteria E. coli. The different lengths of hydrophobic peptides were attached to the C-terminus of the hen egg white lysozyme to investigate the most effective length of the hydrophobic peptides for killing ba...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01071-5
更新日期:1997-09-22 00:00:00
abstract::The cDNA coding for a novel member of the cysteine-rich protein family was isolated from a rat brain cDNA library. It encodes a protein, denoted cysteine-rich protein 2 (CRP2), of 208 amino acid residues containing two tandem repeats of an unusual LIM/double zinc-finger-like motif. The ubiquitous tissue distribution a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80373-3
更新日期:1993-10-25 00:00:00
abstract::Thimet oligopeptidase (EC 3.4.24.15; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00548-9
更新日期:2003-06-19 00:00:00
abstract::Autophagy in the yeast is similar to that in mammalian cells. A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain. In the apg1 strain, which has normal vacuolar proteinas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80398-e
更新日期:1993-10-25 00:00:00
abstract::From human PBMC stimulated with PHA, we have isolated cDNA clones encoding a novel cytokine named SCM-1, which is significantly related to the CC and the CXC chemokines but has only the 2nd and the 4th of the four cysteines conserved in these proteins. Its gene is also distinctly mapped to human chromosome 1. SCM-1 is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00093-o
更新日期:1995-02-27 00:00:00
abstract::Apoptosis is a physiological process for active cell removal. One of its hallmarks is an increased cytosolic Ca2+ content. Several genes involved in apoptosis control have been identified, but their mode of action is not understood in detail. Apoptosis may relate to oncogenesis, in that some malignant tumors may grow ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(93)81423-w
更新日期:1993-06-28 00:00:00
abstract::Leucine-rich repeats (LRRs) are 22-28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide deri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81110-t
更新日期:1991-10-07 00:00:00
abstract::We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825-2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans. The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80609-m
更新日期:1990-02-26 00:00:00
abstract::A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest kno...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02253-5
更新日期:2000-12-08 00:00:00
abstract::Multidrug transporters are involved in mediating the failure of chemotherapy in treating several serious diseases. The archetypal multidrug transporter P-glycoprotein (P-gp) confers resistance to a large number of chemically and functionally unrelated anti-cancer drugs by mediating efflux from cancer cells. The abilit...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.11.083
更新日期:2006-02-13 00:00:00
abstract::Although initially viewed as housekeeping enzymes, research over the last 15 years has revealed that the protein tyrosine phosphatases (PTPs) are critical regulators of tyrosine phosphorylation-dependent signaling events and may represent novel targets for therapeutic intervention in a variety of human diseases. In th...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(03)00603-3
更新日期:2003-07-03 00:00:00
abstract::Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81138-8
更新日期:1985-02-11 00:00:00
abstract::Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00269-0
更新日期:1999-03-19 00:00:00
abstract::PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00877-6
更新日期:1997-08-18 00:00:00
abstract::The sequence of the HIV Nef protein has no significant homology to other proteins in the SwissProt database, and experimental data concerning its function are sparse and contradictory. Using a novel protein sequence comparison method, we find similarities between different Nef sequences and the alpha chain of human MH...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80655-e
更新日期:1993-11-01 00:00:00
abstract::Many proteins located in the intermembrane space (IMS) of mitochondria are characterized by a low molecular mass, contain highly conserved cysteine residues and coordinate metal ions. Studies on one of these proteins, Tim13, revealed that net translocation across the outer membrane is driven by metal-dependent folding...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.072
更新日期:2005-01-03 00:00:00