Abstract:
:Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into the region 163-176 of the parathyroid hormone receptor, and find that crosslinking occurs to the side-chain of methionine over a range of 11 amino acids. We call this the "Magnet Effect" of methionine. Hence, crosslinking contact points can be significantly shifted by the presence of methionine in a receptor domain.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wittelsberger A,Thomas BE,Mierke DF,Rosenblatt Mdoi
10.1016/j.febslet.2006.02.050keywords:
subject
Has Abstractpub_date
2006-03-20 00:00:00pages
1872-6issue
7eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00251-1journal_volume
580pub_type
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