Methionine acts as a "magnet" in photoaffinity crosslinking experiments.

Abstract:

:Photoaffinity crosslinking has been utilized to probe the nature of the ligand-receptor interface for a number of G protein-coupled receptor systems. Often the photoreactive benzophenone moiety incorporated in the ligand is found to react with a methionine in the receptor. We introduced methionines one-at-a-time into the region 163-176 of the parathyroid hormone receptor, and find that crosslinking occurs to the side-chain of methionine over a range of 11 amino acids. We call this the "Magnet Effect" of methionine. Hence, crosslinking contact points can be significantly shifted by the presence of methionine in a receptor domain.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Wittelsberger A,Thomas BE,Mierke DF,Rosenblatt M

doi

10.1016/j.febslet.2006.02.050

keywords:

subject

Has Abstract

pub_date

2006-03-20 00:00:00

pages

1872-6

issue

7

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(06)00251-1

journal_volume

580

pub_type

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