Glutamate-101 is critical for the function of the sodium and chloride-coupled GABA transporter GAT-1.

abstract：:Leucine-rich repeats (LRRs) are 22-28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide deri...

journal_title：FEBS letters

authors： Gay NJ,Packman LC,Weldon MA,Barna JC

更新日期：1991-10-07 00:00:00

abstract：:The number of synthetic UTP analogues containing methyl groups in different positions of the ribose moiety were tested as substrates for T7 RNA polymerase (T7 RNAP). Two of these compounds (containing substituents in the 5' position) were shown to be weak substrates of T7 RNAP. 3'Me-UTP was neither substrate nor inhib...

journal_title：FEBS letters

authors： Tunitskaya VL,Rusakova EE,Padyukova NSh,Ermolinsky BS,Chernyi AA,Kochetkov SN,Lysov YuP,Mikhailov SN

更新日期：1997-01-06 00:00:00

abstract：:DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of d...

journal_title：FEBS letters

authors： Wang Y,Rea T,Bian J,Gray S,Sun Y

更新日期：1999-02-26 00:00:00

abstract：:Ghrelin is a novel gastrointestinal peptide that stimulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such negative energy states as starvation and low body weight. We investigated the dynamics of ghrelin in rats with streptozotocin-induced diabetes, ...

journal_title：FEBS letters

authors： Masaoka T,Suzuki H,Hosoda H,Ota T,Minegishi Y,Nagata H,Kangawa K,Ishii H

更新日期：2003-04-24 00:00:00

abstract：:The role of TlyA, TlyB and TlyC proteins in the biology of Leptospira is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed ...

journal_title：FEBS letters

authors： Carvalho E,Barbosa AS,Gómez RM,Cianciarullo AM,Hauk P,Abreu PA,Fiorini LC,Oliveira ML,Romero EC,Gonçales AP,Morais ZM,Vasconcellos SA,Ho PL

更新日期：2009-04-17 00:00:00

abstract：:Disruption of yeast spheroplasts by DEAE-dextran in isoosmotic conditions allows isolation of relatively undamaged subcellular fractions from yeast. The preservation of mitochondria and vacuoles permits the simultaneous isolation of the cytosol with negligible contamination by vacuolar proteinases and therefore, virtu...

journal_title：FEBS letters

authors： Schwencke J,Canut H,Flores A

更新日期：1983-06-13 00:00:00

abstract：:Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus, and characterized. Together with Pi1 and HsTx1, MTX belongs to a family of short-chain four-disulfide-bridged scorpion toxins acting on potassium channels. However, contrary to other members ...

journal_title：FEBS letters

authors： Carlier E,Fajloun Z,Mansuelle P,Fathallah M,Mosbah A,Oughideni R,Sandoz G,Di Luccio E,Geib S,Regaya I,Brocard J,Rochat H,Darbon H,Devaux C,Sabatier JM,de Waard M

更新日期：2001-02-02 00:00:00

abstract：:The mechanisms that underlie axon formation are still poorly understood. GSK3 has been recently implicated in establishing the axon and in its elongation. We have used four different GSK3 inhibitors to determine the role of GSK3 activity in hippocampal neurons at different periods of time. Inhibition of GSK3 activity ...

journal_title：FEBS letters

authors： Garrido JJ,Simón D,Varea O,Wandosell F

更新日期：2007-04-17 00:00:00

abstract：:Partially reduced metabolites of molecular oxygen, superoxide (O2-) and hydrogen peroxide (H2O2), are detected in respiratory tract lining fluid, and it is assumed that these are key components of innate immunity. Whether these reactive oxygen species (ROS) are produced specifically by the respiratory epithelium in re...

journal_title：FEBS letters

authors： Harper RW,Xu C,Eiserich JP,Chen Y,Kao CY,Thai P,Setiadi H,Wu R

更新日期：2005-08-29 00:00:00

abstract：:We have used a competitive enzyme-linked immunoassay with a panel of monoclonal antibodies to probe the topography of the membrane-bound form of apolipoprotein B (apo B) in rabbit microsomes. All epitopes investigated were found to be expressed at the cytosolic side of the microsomal membrane under conditions in which...

journal_title：FEBS letters

authors： Wilkinson J,Higgins JA,Groot PH,Gherardi E,Bowyer DE

更新日期：1992-06-08 00:00:00

abstract：:Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...

journal_title：FEBS letters

authors： Hornstein E,Harel H,Levy G,Meyuhas O

更新日期：1999-08-27 00:00:00

abstract：:The long form of human uncoupling protein-3 (hUCP3L) is highly homologous to thermogenin (UCPI), the uncoupling protein of brown fat mitochondria, but is expressed predominantly in skeletal muscle. Its putative role is to regulate the coupling efficiency of oxidative phosphorylation and thus thermogenesis in skeletal ...

journal_title：FEBS letters

authors： Hinz W,Faller B,Grüninger S,Gazzotti P,Chiesi M

更新日期：1999-04-01 00:00:00

abstract：:Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified gamma-secretase, which cleaves the membrane-bound beta-amyloid precursor protein (betaAPP) at th...

journal_title：FEBS letters

authors： Steiner H,Pesold B,Haass C

更新日期：1999-12-17 00:00:00

abstract：:During lactation glucose metabolism in paraovarian adipocytes is characterized by a 40 and 80% decrease of glucose incorporation into CO2 and fatty acids in the presence of insulin. In contrast with the stimulation by insulin of glucose incorporation into lactate, glycerol remains unchanged. As a result, insulin sensi...

journal_title：FEBS letters

authors： Burnol AF,Guerre-Millo M,Lavau M,Girard J

更新日期：1986-01-06 00:00:00

abstract：:Patients suffering from Refsum disease have a defect in the alpha-oxidation pathway which results in the accumulation of phytanic acid in plasma and tissues. Our previous studies have shown that phytanic acid is also a substrate for the omega-oxidation pathway. With the use of specific inhibitors we now show that memb...

journal_title：FEBS letters

authors： Komen JC,Wanders RJ

更新日期：2006-07-10 00:00:00

abstract：:Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this li...

journal_title：FEBS letters

authors： Blaner WS,Bonifácio MJ,Feldman HD,Piantedosi R,Saraiva MJ

更新日期：1991-08-05 00:00:00

abstract：:The effect of removal of Ca2+ bound to thrombospondin (TSP) on the protein structure in solution has been investigated using ESR spin-label techniques. A maleimide spin label was selectively attached to the free thiol group presumably near the carboxyl-terminal domain in which Ca2+-binding sites are situated. The ESR ...

journal_title：FEBS letters

authors： Slane JM,Mosher DF,Lai CS

更新日期：1988-03-14 00:00:00

abstract：:Creatine kinases (CK) play a prominent role in cell energy distribution through an energy shuttle between mitochondria and other organelles. Human brain CK was cloned and overexpressed in COS-7 cells. We then deleted His-65 and/or Pro-66 situated near the center of a flexible loop as shown by X-ray crystallography on ...

journal_title：FEBS letters

authors： Mourad-Terzian T,Steghens JP,Min KL,Collombel C,Bozon D

更新日期：2000-06-09 00:00:00

abstract：:We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...

journal_title：FEBS letters

authors： Sablok G,Milev I,Minkov G,Minkov I,Varotto C,Yahubyan G,Baev V

更新日期：2013-08-19 00:00:00

abstract：:Membrane proteins are usually solubilized in polar solvents by incorporation into micelles. Even for small membrane proteins these mixed micelles have rather large molecular masses, typically beyond 50000 Da. The NMR technique TROSY (transverse relaxation-optimized spectroscopy) has been developed for studies of struc...

journal_title：FEBS letters

authors： Fernández C,Hilty C,Bonjour S,Adeishvili K,Pervushin K,Wüthrich K

更新日期：2001-08-31 00:00:00

abstract：:We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...

journal_title：FEBS letters

authors： Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

更新日期：2016-09-01 00:00:00

abstract：:Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stabilit...

journal_title：FEBS letters

authors： Sivakumar N,Li N,Tang JW,Patel BK,Swaminathan K

更新日期：2006-05-15 00:00:00

abstract：:Friedreich's ataxia is caused by a deficit in the mitochondrial protein frataxin. The present work demonstrates that in vivo yeast frataxin Yfh1p and Isu1p, the mitochondrial scaffold protein for the Fe-S cluster assembly, have tightly linked biological functions, acting in concert to promote the Fe-S cluster assembly...

journal_title：FEBS letters

authors： Ramazzotti A,Vanmansart V,Foury F

更新日期：2004-01-16 00:00:00

abstract：:Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y-A bonds of single-stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonu...

journal_title：FEBS letters

authors： Stevens A

更新日期：1986-09-15 00:00:00

abstract：:Using a cationic lipid bilayer, we show that DNA can be reliably adsorbed to the bilayer surface for atomic force microscopy (AFM) in aqueous buffers at high resolution. The measured width of the dsDNA is close to 2 nm, and a periodic modulation on dsDNA is reproducibly detected by the AFM. The measured period is 3.4 ...

journal_title：FEBS letters

authors： Mou J,Czajkowsky DM,Zhang Y,Shao Z

更新日期：1995-09-11 00:00:00

abstract：:In the bovine ovary there is a delay of 4-6 days between the observed maximum of oxytocin mRNA and the peak in the luteal levels of oxytocin nonapeptide. This implies a maturation process involving components of the post-translational processing pathway. In situ hybridization shows the oxytocin gene to be transcribed ...

journal_title：FEBS letters

authors： Fehr S,Ivell R,Koll R,Schams D,Fields M,Richter D

更新日期：1987-01-01 00:00:00

abstract：:A Trp-free alphaA-crystallin mutant (W9F) was prepared by site-directed mutation. This mutant appears to be identical to the wild-type in terms of conformation (secondary and tertiary structures). W9F was labeled with a sulfhydryl-specific fluorescent probe, 2-(4'-maleimidylanilino) naphthalene-6-sulfonate (MIANS), an...

journal_title：FEBS letters

authors： Sun TX,Akhtar NJ,Liang JJ

更新日期：1998-07-03 00:00:00

abstract：:Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis dem...

journal_title：FEBS letters

authors： Zhou L,Chen X,Shao Z,Zhou P,Knight DP,Vollrath F

更新日期：2003-11-20 00:00:00

abstract：:Diapause is an important overwintering strategy enabling Locusta migratoria to survive under stressed conditions. We identified a novel dual-specificity kinase gene that is differentially expressed between long and short day-treated L. migratoria. To determine its function on photoperiodic diapause induction, we clone...

journal_title：FEBS letters

authors： Hao K,Ullah H,Jarwar AR,Nong X,Tu X,Zhang Z

更新日期：2019-11-01 00:00:00

abstract：:VirG, PhoB, and OmpR are positive regulators of the virulence, phosphate, and osmolarity regulons, respectively. Their target genes are attended with one or more significant blocks of similar 6-bp sequences in their regulatory regions. The distance between the block(s) and transcriptional start site varies with the ta...

journal_title：FEBS letters

authors： Aoyama T,Oka A

更新日期：1990-04-09 00:00:00