Abstract:
:Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified gamma-secretase, which cleaves the membrane-bound beta-amyloid precursor protein (betaAPP) at the C-terminus of its amyloid domain within the membrane to release the neurotoxic amyloid beta-peptide. In order to allow the isolation of proteases, which specifically cleave membrane-bound substrates within or in the vicinity of a transmembrane domain, we developed a reporter gene assay in Saccharomyces cerevisiae. This assay may allow the identification of genes encoding target proteases that specifically cleave membrane bound substrates by transforming expression libraries.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Steiner H,Pesold B,Haass Cdoi
10.1016/s0014-5793(99)01627-0keywords:
subject
Has Abstractpub_date
1999-12-17 00:00:00pages
245-9issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01627-0journal_volume
463pub_type
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