Mitochondrial alterations induced by aspirin in rat hepatocytes expressing mitochondrially targeted green fluorescent protein (mtGFP).


:Mitochondria in primary living hepatocytes were visualized in cells transfected with a chimeric plasmid encoding for the green fluorescent protein (GFP) of Aequorea victoria engineered to be specifically targeted to mitochondria, as described recently (Rizutto et al. (1995) Curr. Biol. 5, 635-642). The identification of the fluorescent organelles as authentic mitochondria was confirmed by double labeling with rhodamine 123. Acetylsalicylate treatment of hepatocytes induced in mitochondria typical morphological alterations closely analogous to the swelling promoted by acetylsalicylate in isolated mitochondria. Cyclosporin A, which in isolated mitochondria prevents the changes induced by acetylsalicylate, had no protective action but induced per se specific alterations in the morphology of mitochondria. Moreover, exposure of hepatocytes to cyclosporin A followed by acetylsalicylate caused the same mitochondrial changes induced by each of the two compounds separately. The structural alterations caused by acetylsalicylate were constantly associated with a decrease in mitochondrial urea synthesis and cell viability.




FEBS letters


Venerando R,Miotto G,Pizzo P,Rizzuto R,Siliprandi N




Has Abstract


1996-03-18 00:00:00














  • Human CyP33 binds specifically to mRNA and binding stimulates PPIase activity of hCyP33.

    abstract::Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang Y,Han R,Zhang W,Yuan Y,Zhang X,Long Y,Mi H

    更新日期:2008-03-05 00:00:00

  • Ground state isomerization of a model green fluorescent protein chromophore.

    abstract::The relationship between ground state cis-trans isomerization and protonation state is explored for a model green fluorescent protein chromophore, 4-hydroxybenzylidene-1,2-dimethylimidazolinone (HBDI). We find that the protonation state has only a modest effect on the free energy differences between cis and trans isom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: He X,Bell AF,Tonge PJ

    更新日期:2003-08-14 00:00:00

  • RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs have identical stability.

    abstract::Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sugimoto N,Katoh M,Nakano S,Ohmichi T,Sasaki M

    更新日期:1994-10-31 00:00:00

  • The role of long-chain acyl-CoA in the damage of oxidative phosphorylation in heart mitochondria.

    abstract::The aim of this investigation was to study the effect of intramitochondrial acyl-CoA on the respiration of rabbit heart mitochondria over the whole range of stationary respiratory rates between States 4 and 3. The creatine phosphokinase system was used for stabilization of extramitochondrial adenine nucleotide concent...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Borutaite V,Mildaziene V,Ivanoviene L,Kholodenko B,Toleikis A,Praskevicius A

    更新日期:1989-01-30 00:00:00

  • Stimulation of mitogen activated protein kinase by LDL and oxLDL in human U-937 macrophage-like cells.

    abstract::Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 microgram/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Deigner HP,Claus R

    更新日期:1996-05-06 00:00:00

  • Identification of an uncoupling mutation affecting the b subunit of F1F0 ATP synthase in Escherichia coli.

    abstract::A specific b subunit arginine, b(Arg-36) in Escherichia coli, displays evolutionary conservation among bacterial F1F0 ATP synthases. Site-directed mutagenesis was used to generate a collection of mutations affecting b(Arg-36). The phenotype differed depending upon the substitution, and the b(Arg-36-Glu) and b(Arg-36-I...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Caviston TL,Ketchum CJ,Sorgen PL,Nakamoto RK,Cain BD

    更新日期:1998-06-12 00:00:00

  • Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem-resistant host.

    abstract::Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoneyama H,Nakae T

    更新日期:1991-06-03 00:00:00

  • Selection for TRAIL resistance results in melanoma cells with high proliferative potential.

    abstract::To better understand the outcome of the interaction between TNF-related apoptosis-inducing factor (TRAIL) and tumor cells, we studied TRAIL-resistant melanoma cells resulting from prolonged exposure to TRAIL and found that they had higher proliferative activity than the parental cells both in vitro and in vivo. This w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wu JJ,Zhang XD,Gillespie S,Hersey P

    更新日期:2005-03-28 00:00:00

  • Evidence for release of free glucose from muscle during amylin-induced glycogenolysis in rats.

    abstract::Amylin, a 37 amino acid product of pancreatic beta-cells, inhibits insulin-stimulated radioglucose incorporation into muscle glycogen. In the current study, we exercised rats and then prelabelled the glycogen pool by infusing [3-3H]glucose along with glucose and insulin. Subsequent amylin administration increased the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Young AA,Carlo P,Smith P,Wolfe-Lopez D,Pittner R,Wang MW,Rink T

    更新日期:1993-11-22 00:00:00

  • A new family of bacterial DNA repair proteins annotated by the integration of non-homology, distant homology and structural bioinformatic methods.

    abstract::Different bioinformatics methods illuminate different aspects of protein function, from specific catalytic activities to broad functional categories. Here, a triple-pronged approach to predict function for a domain of unknown function, DUF2086, is applied. Distant homology to characterised enzymes and conservation of ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mello LV,Rigden DJ

    更新日期:2012-11-02 00:00:00

  • A comparative picosecond-resolved fluorescence study of tryptophan residues in iron-sulfur proteins.

    abstract::The fluorescence intensity and anisotropy decays of the intrinsic tryptophan emission from six Fe/S proteins (ranging from the very simplest ones to enzyme complexes containing one, two or more Trp residues) were measured. All proteins were examined in the reduced and the oxidized state. In either redox state each pro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dorovska-Taran V,van Hoek A,Link TA,Visser AJ,Hagen WR

    更新日期:1994-07-18 00:00:00

  • The yeast metacaspase is implicated in oxidative stress response in frataxin-deficient cells.

    abstract::Friedreich ataxia is the most common recessive neurodegenerative disease and is caused by reduced expression of mitochondrial frataxin. Frataxin depletion causes impairment in iron-sulfur cluster and heme biosynthesis, disruption of iron homeostasis and hypersensitivity to oxidants. Currently no pharmacological treatm...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lefevre S,Sliwa D,Auchère F,Brossas C,Ruckenstuhl C,Boggetto N,Lesuisse E,Madeo F,Camadro JM,Santos R

    更新日期:2012-01-20 00:00:00

  • The pathway of CO binding to cytochrome c oxidase. Can the gateway be closed?

    abstract::Addition of cyanide to the CO complex of cytochrome oxidase reduces the apparent photosensitivity of the Fe-CO bond. This effect is not seen with azide, or when cyanide is added to ferromyoglobin-CO. It is proposed that cyanide binds to CuB, and restricts the passage of CO out of the protein. This restriction favors g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hill BC

    更新日期:1994-11-14 00:00:00

  • Protective role for cytosolic phospholipase A2alpha in autoimmune diabetes of mice.

    abstract::Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) plays an important role in arachidonate pathway. To investigate the contribution of cPLA(2)alpha to autoimmune diabetes, we established non-obese diabetic (NOD) mouse, an excellent model for human type 1 diabetes, deficient in cPLA(2)alpha. These mice showed severe insu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oikawa Y,Yamato E,Tashiro F,Yamamoto M,Uozumi N,Shimada A,Shimizu T,Miyazaki J

    更新日期:2005-07-18 00:00:00

  • Increased muscarinic receptor binding in heart membranes by an inhibitor of protein kinase C.

    abstract::The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with poly...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Creazzo TL,Wrenn RW

    更新日期:1988-12-19 00:00:00

  • Specific binding of protein kinase CK2 catalytic subunits to tubulin.

    abstract::Protein kinase CK2 is composed of two regulatory beta-subunits and two catalytic alpha- or alpha'-subunits. To analyse these subunits individually we generated antibodies against unique peptides derived from the alpha-, alpha'- and beta-subunit. Immunofluorescence studies with these antibodies revealed the presence of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Faust M,Schuster N,Montenarh M

    更新日期:1999-11-26 00:00:00

  • Mutations in the glucose-6-phosphate transporter (G6PT) gene in patients with glycogen storage diseases type 1b and 1c.

    abstract::Glycogen storage diseases type 1 (GSD 1) are a group of autosomal recessive disorders characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Mutations of the glucose-6-phosphatase gene are responsible for the most frequent form of GSD 1, the subtype 1a, while mutations of the glucose-6-ph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Galli L,Orrico A,Marcolongo P,Fulceri R,Burchell A,Melis D,Parini R,Gatti R,Lam C,Benedetti A,Sorrentino V

    更新日期:1999-10-08 00:00:00

  • Prominent expression of FRS2beta protein in neural cells and its association with intracellular vesicles.

    abstract::The fibroblast growth factor receptor substrate (FRS)-2 protein family comprises FRS2alpha, a well-known central mediator for fibroblast growth factor signaling, and FRS2beta, whose endogenous expression pattern and function are not yet defined. Immunohistochemical analysis revealed that expression of FRS2beta was res...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Minegishi Y,Iwanari H,Mochizuki Y,Horii T,Hoshino T,Kodama T,Hamakubo T,Gotoh N

    更新日期:2009-02-18 00:00:00

  • X-linked inhibitor of apoptosis protein mediates neddylation by itself but does not function as a NEDD8-E3 ligase for caspase-7.

    abstract::X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

    更新日期:2012-06-04 00:00:00

  • Functional diversity along the transverse axis of hippocampal area CA1.

    abstract::Decades of neuroscience research have shed light on the hippocampus as a key structure for the formation of episodic memory. The hippocampus is divided into distinct subfields - CA1, CA2 and CA3. While accumulating evidence points to cellular and synaptic heterogeneity within each subfield, this heterogeneity has not ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Igarashi KM,Ito HT,Moser EI,Moser MB

    更新日期:2014-08-01 00:00:00

  • Farnesyl-diphosphate farnesyltransferase 1 regulates hepatitis C virus propagation.

    abstract::To identify the novel genes involved in lipid metabolism and lipid droplet formation that may play important roles in Hepatitis C virus (HCV) propagation, we have screened the small interfering RNA library using cell culture derived HCV (HCVcc)-infected cells. We selected and characterized the gene encoding farnesyl-d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Park EM,Nguyen LN,Lim YS,Hwang SB

    更新日期:2014-05-02 00:00:00

  • GenAge: a genomic and proteomic network map of human ageing.

    abstract::The aim of this work was to provide an overview of the genetics of human ageing to gain novel insights about the mechanisms involved. By incorporating findings from model organisms to humans, such as mutations that either delay or accelerate ageing in mice, we constructed the gene networks previously related to ageing...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de Magalhães JP,Toussaint O

    更新日期:2004-07-30 00:00:00

  • Amino acid sequence of human plasma apolipoprotein C-III from normolipidemic subjects.

    abstract::The complete amino acid sequence of human plasma apolipoprotein C-III (apoC-III) isolated from normal subjects is described. ApoC-III is a linear polypeptide chain of 79 amino acids. Tryptic digestion of intact apoC-III produced 5 major peptides, while tryptic digestion of the citraconylated protein yielded two peptid...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hospattankar AV,Brewer HB Jr,Ronan R,Fairwell T

    更新日期:1986-03-03 00:00:00

  • Influenza A virus-induced caspase-3 cleaves the histone deacetylase 6 in infected epithelial cells.

    abstract::Histone deacetylase 6 (HDAC6) is a multi-substrate cytoplasmic enzyme that regulates many important biological processes. Recently, some reports have implicated HDAC6 in viral infection. However, nothing is known about its regulation in virus-infected cells. The data presented here for the first time demonstrate the c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Husain M,Harrod KS

    更新日期:2009-08-06 00:00:00

  • MDM2 interacts with MDMX through their RING finger domains.

    abstract::The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo M

    更新日期:1999-03-19 00:00:00

  • Identification of genes encoding microbial glucuronoyl esterases.

    abstract::One type of covalent linkages connecting lignin and hemicellulose in plant cell walls is the ester linkage between 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols. An enzyme that could hydrolyze such linkages, named glucuronoyl esterase, occurs in the cellulolytic system of the wood-rotting fungus S...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li XL,Spániková S,de Vries RP,Biely P

    更新日期:2007-08-21 00:00:00

  • Cloning, sequencing and expression of a full-length rabbit fast skeletal troponin-C cDNA.

    abstract::A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed prote...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chen Q,Taljanidisz J,Sarkar S,Tao T,Gergely J

    更新日期:1988-02-08 00:00:00

  • Crenactin from Pyrobaculum calidifontis is closely related to actin in structure and forms steep helical filaments.

    abstract::Polymerising proteins of the actin family are nearly ubiquitous. Crenactins, restricted to Crenarchaea, are more closely related to actin than bacterial MreB. Crenactins occur in gene clusters hinting at an unknown, but conserved function. We solved the crystal structure of crenactin at 3.2 Å resolution. The protein c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Izoré T,Duman R,Kureisaite-Ciziene D,Löwe J

    更新日期:2014-03-03 00:00:00

  • CLC transport proteins in plants.

    abstract::Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO(3)(-)/H(+) antiporter critical for nitrate transpo...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Zifarelli G,Pusch M

    更新日期:2010-05-17 00:00:00

  • SV40 chromatin structure is not essential for viral gene expression.

    abstract::The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Graessmann A,Bumke-Vogt C,Buschhausen G,Bauer M,Graessmann M

    更新日期:1985-01-01 00:00:00