Mitochondrial alterations induced by aspirin in rat hepatocytes expressing mitochondrially targeted green fluorescent protein (mtGFP).

abstract：:The bcl-2 gene is an important antagonist of apoptosis, the programmed cell death. Bcl-2 is highly expressed in a variety of lymphomas. Lymphocytes of patients with chronic lymphocytic leukemia (CLL) express high amounts of bcl-2 even in the absence of the t(14;18) translocation, resulting in a strong resistance towar...

journal_title：FEBS letters

authors： Körner I,Weber-Nordt R,Pfaff P,Finke J

更新日期：1997-04-07 00:00:00

abstract：:Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RIAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first...

journal_title：FEBS letters

authors： McGregor GP,Hartel R,Fehmann HC,Lankat-Buttgereit B,Göke B,Göke R,Voigt K

更新日期：1992-11-09 00:00:00

abstract：:Na+ transport through the F0 domain of Na(+)-F1F0-ATPases involves the combined action of subunits c and a but the residues involved in Na+ liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na(+)-F1F0-ATPase...

journal_title：FEBS letters

authors： Rahlfs S,Müller V

更新日期：1999-06-18 00:00:00

abstract：:We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of ...

journal_title：FEBS letters

authors： Macieira-Coelho A

更新日期：1995-01-23 00:00:00

abstract：:We previously showed that autocrine tumor necrosis factor (TNF) production in the TNF-sensitive L929sA fibrosarcoma cell line induced TNF resistance, which is correlated with downmodulation of both TNF receptors on the cell surface. We now analyzed whether autocrine TNF production also interfered with intracellular TN...

journal_title：FEBS letters

authors： Decoster E,Vandevoorde V,Beyaert R,Haegeman G,Fiers W

更新日期：1997-10-20 00:00:00

abstract：:Uptake of taurine in human placental brush-border membrane vesicles was greatly stimulated in the presence of an inwardly-directed Na+ + Cl- -gradient and uphill transport of taurine could be demonstrated under these conditions. Na+ as well as Cl- were obligatory for this uptake and both ion gradients could energize t...

journal_title：FEBS letters

authors： Miyamoto Y,Balkovetz DF,Leibach FH,Mahesh VB,Ganapathy V

更新日期：1988-04-11 00:00:00

abstract：:Based on imino proton exchange catalysis, base-pair lifetimes and apparent dissociation constants are reported on the complexes formed by bisintercalation of echinomycin at the CpG steps of the d(ACGTATACGT)2 and d(ACGTTAACGT)2 duplexes. The lifetimes of the four central A x T base pairs between two echinomycin bindin...

journal_title：FEBS letters

authors： Park JY,Lee JH,Choi BS

更新日期：1998-04-24 00:00:00

abstract：:A macromolecular complex containing survival of motor neurons (SMN), the spinal muscular atrophy protein, and Gemin2-7 interacts with Sm proteins and snRNAs to carry out the assembly of these components into spliceosomal small nuclear ribonucleoproteins (snRNPs). Here we report the characterization of unr-interacting ...

journal_title：FEBS letters

authors： Carissimi C,Baccon J,Straccia M,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L

更新日期：2005-04-25 00:00:00

abstract：:We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...

journal_title：FEBS letters

authors： Romero LC,Biswal B,Song PS

更新日期：1991-05-06 00:00:00

abstract：:A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and ...

journal_title：FEBS letters

authors： Tagaya M,Henomatsu N,Yoshimori T,Yamamoto A,Tashiro Y,Fukui T

更新日期：1993-06-14 00:00:00

abstract：:Using a panel of monoclonal antibodies, it has previously been demonstrated that the cytosol of nucleated red cells (trout and turkey) contains a protein similar to arrestin, a soluble protein found so far only in the photosensitive cells and which, by binding to photoexcited rhodopsin, inhibits the phototransduction ...

journal_title：FEBS letters

authors： Scheuring U,Franco M,Fievet B,Guizouarn H,Mirshahi M,Faure JP,Motais R

更新日期：1990-12-10 00:00:00

abstract：:Acylaminoacyl peptidase is a member of the prolyl oligopeptidase family. Amino acid sequence alignment suggests that the stabilization of the tetrahedral intermediate should be mediated by His507 rather than by a tyrosine residue found in the other family members of this serine peptidase group. The pH dependence of k(...

journal_title：FEBS letters

authors： Kiss AL,Szeltner Z,Fülöp V,Polgár L

更新日期：2004-07-30 00:00:00

abstract：:Preillumination of intact cells of the eukaryotic, halotolerant, cell-wall-less green alga Dunaliella salina induces a dark ATPase activity the magnitude of which is about 3-5-fold higher than the ATPase activity observed in dark-adapted cells. The light-induced activity arises from the activation and stabilization in...

journal_title：FEBS letters

authors： Selman-Reimer S,Selman BR

更新日期：1988-03-28 00:00:00

abstract：:14-3-3s predominantly form homo-/heterodimers that are in equilibrium with corresponding monomers. Dimer/monomer equilibrium depends on the nature and phosphorylation of Ser58 of certain 14-3-3 isoforms. The structure and properties of 14-3-3 dimers are well characterized, whereas 14-3-3 monomers are less investigated...

journal_title：FEBS letters

authors： Sluchanko NN,Gusev NB

更新日期：2012-12-14 00:00:00

abstract：:The mutation of Asp198 to Asn in the receptor for glucagon-like peptide-1(7-36)amide (GLP-1) had no effect upon GLP-1 affinity whereas substitution with Ala greatly reduced affinity, demonstrating the importance of polarity rather than negative charge at Asp198. However, the Asp198-Ala mutation had less effect upon th...

journal_title：FEBS letters

authors： López de Maturana R,Donnelly D

更新日期：2002-10-23 00:00:00

abstract：:Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...

journal_title：FEBS letters

authors： Yu J,Jia Y,Guo Y,Chang G,Duan W,Sun M,Li B,Li C

更新日期：2010-07-02 00:00:00

abstract：:Prolonged incubation of membrane fragments containing homogeneous (Na+ + K+)-ATPase with Mg2+, K+ and VO-3 at 4 degrees C resulted in formation of two-dimensional crystals of this enzyme with unit cell parameters: a = 66 A, b = 118 A, gamma = 108 degrees. The crystals correspond to the two-sided plane group p21. By co...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Demin VV,Barnakov AN,Kuzin AP,Lunev AV,Modyanov NN,Dzhandzhugazyan KN

更新日期：1985-10-07 00:00:00

abstract：:We provide first large scale analysis of the peculiarities of surface areas of 5658 dissimilar (below 50% sequence similarity) proteins with known 3D-structures that bind to proteins, DNA or RNAs. We show here that area of the protein surface is highly correlated with the protein length. The size of the interface surf...

journal_title：FEBS letters

authors： Wu Z,Hu G,Yang J,Peng Z,Uversky VN,Kurgan L

更新日期：2015-09-14 00:00:00

abstract：:Stroke is characterized by massive inflammation in areas surrounding the injury that magnifies damage to the brain. The liver X receptors (LXRs) are nuclear receptors that regulate cholesterol, lipid, and glucose metabolism. Synthetic LXR agonists have potent anti-inflammatory properties in a variety of settings, incl...

journal_title：FEBS letters

authors： Sironi L,Mitro N,Cimino M,Gelosa P,Guerrini U,Tremoli E,Saez E

更新日期：2008-10-15 00:00:00

abstract：:The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...

journal_title：FEBS letters

authors： Olgemöller B,Schleicher ED,Schwaabe S,Guretzki HJ,Gerbitz KD

更新日期：1990-05-07 00:00:00

abstract：:Saturable binding sites for the labelled calcium antagonist (+/-)[3H]nimodipine were found in guinea-pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd = 1.5 +/- 0.03 nM, Bmax = 2.1 +/- 0.25 pmol/pro...

journal_title：FEBS letters

authors： Ferry DR,Glossmann H

更新日期：1982-11-08 00:00:00

abstract：:Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic c...

journal_title：FEBS letters

authors： Samaddar S,Dutta A,Sinharoy S,Paul A,Bhattacharya A,Saha S,Chien KY,Goshe MB,DasGupta M

更新日期：2013-09-17 00:00:00

abstract：:The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwe...

journal_title：FEBS letters

authors： Katayama K,Dobashi Y,Kitagawa M,Kamekura S,Kawai M,Kadoya Y,Kameya T

更新日期：2001-12-14 00:00:00

abstract：:Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two p...

journal_title：FEBS letters

authors： Moonen CT,Hore PJ,Müller F,Kaptein R,Mayhew SG

更新日期：1982-11-22 00:00:00

abstract：:Measurement of the weight of desmosterol produced during its biosynthesis in the presence of tritiated water and triparanol has permitted a direct determination of the relative flux of carbon and tritium (the H/C ratio) into sterol in hepatocytes. The H/C ratio increased with time of incubation irrespective of the nut...

journal_title：FEBS letters

authors： Björnsson OG,Pullinger CR,Gibbons GF

更新日期：1985-08-05 00:00:00

abstract：:Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

journal_title：FEBS letters

authors： Discenza MT,Vaz D,Hassell JA,Pelletier J

更新日期：2004-02-27 00:00:00

abstract：:To clarify the function of osteopontin in osteoblast differentiation, we have examined the signal transduction pathway in an osteoblastic cell line (UMR106-6) bound to osteopontin, fibronectin, vitronectin and collagen type I surfaces. This was done by investigating the production and autophosphorylation of focal adhe...

journal_title：FEBS letters

authors： Liu YK,Uemura T,Nemoto A,Yabe T,Fujii N,Ushida T,Tateishi T

更新日期：1997-12-22 00:00:00

abstract：:A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

journal_title：FEBS letters

authors： Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

更新日期：1994-03-07 00:00:00

abstract：:Insecticidal proteins or delta-endotoxins of Bacillus thuringiensis are highly toxic to a wide range of agronomically important pests. The toxins are formed of three structural domains. The N-terminal domain is a bundle of eight alpha-helices and is implicated in pore formation in insect midgut epithelial membranes. A...

journal_title：FEBS letters

authors： Chandra A,Ghosh P,Mandaokar AD,Bera AK,Sharma RP,Das S,Kumar PA

更新日期：1999-09-17 00:00:00

abstract：:The cDNA coding for the inducible H+/hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose-induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to in...

journal_title：FEBS letters

authors： Sauer N,Tanner W

更新日期：1989-12-18 00:00:00