Regulation of acetylcholine receptor gene expression in rats treated with alpha-bungarotoxin.


:Regulation of acetylcholine receptor (AChR) gene expression was analyzed in alpha-bungarotoxin (alpha-BTX) treated rats. A reduction in available 125I-alpha-BTX binding sites was accompanied by an increase in the various AChR transcripts. The increase in the AChR alpha-, beta- epsilon- and delta-subunit mRNAs was similar to that observed in rats with experimental autoimmune myasthenia gravis (EAMG). Unlike in EAMG, the gamma-subunit transcripts reappeared following alpha-BTX treatment. The quantitative differences in the levels of AChR transcripts between alpha-BTX treatment and EAMG on one hand and denervation on the other hand, support the notion that the regulation of AChR gene expression is controlled by muscle activity and by neuronal factors as well. We also demonstrate in this report that myogenin transcripts increase following alpha-BTX treatment as well as following denervation, whereas MyoD1 transcripts remain stable.




FEBS letters


Asher O,Provenzano C,Fuchs S





Has Abstract


1991-05-06 00:00:00














  • Reconstruction and in silico analysis of the MAPK signaling pathways in the human blood fluke, Schistosoma japonicum.

    abstract::At present, little is known about signal transduction mechanisms in schistosomes, which cause the disease of schistosomiasis. The mitogen-activated protein kinase (MAPK) signaling pathways, which are evolutionarily conserved from yeast to Homo sapiens, play key roles in multiple cellular processes. Here, we reconstruc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang L,Yang Z,Li Y,Yu F,Brindley PJ,McManus DP,Wei D,Han Z,Feng Z,Li Y,Hu W

    更新日期:2006-06-26 00:00:00

  • ACBD3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b-GAP.

    abstract::Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Yue X,Bao M,Christiano R,Li S,Mei J,Zhu L,Mao F,Yue Q,Zhang P,Jing S,Rothman JE,Qian Y,Lee I

    更新日期:2017-09-01 00:00:00

  • Expression of human apolipoprotein A-I/C-III/A-IV gene cluster in mice reduces atherogenesis in response to a high fat-high cholesterol diet.

    abstract::We have previously generated transgenic (Tg) mice expressing the human apolipoprotein (apo) A-I/C-III/A-IV gene cluster. This expression induced hyperlipidemia but reduced atherosclerotic lesions in genetically modified mice lacking apoE. Atherosclerosis is a multifactorial process and environmental factors such as di...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Baroukh N,Ostos MA,Vergnes L,Recalde D,Staels B,Fruchart J,Ochoa A,Castro G,Zakin MM

    更新日期:2001-07-27 00:00:00

  • The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d-galacturonic acid from pectin.

    abstract::We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that Ga...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Alazi E,Niu J,Kowalczyk JE,Peng M,Aguilar Pontes MV,van Kan JA,Visser J,de Vries RP,Ram AF

    更新日期:2016-06-01 00:00:00

  • Activation of tyrosine kinase of EGFR induces Gbetagamma-dependent GRK-EGFR complex formation.

    abstract::This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. T...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gao J,Li J,Chen Y,Ma L

    更新日期:2005-01-03 00:00:00

  • X-linked inhibitor of apoptosis protein mediates neddylation by itself but does not function as a NEDD8-E3 ligase for caspase-7.

    abstract::X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

    更新日期:2012-06-04 00:00:00

  • Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria.

    abstract::A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold hig...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sörensen M,Lippuner C,Kaiser T,Misslitz A,Aebischer T,Bumann D

    更新日期:2003-09-25 00:00:00

  • Adrenomedullin protects against hypoxia/reoxygenation-induced cell death by suppression of reactive oxygen species via thiol redox systems.

    abstract::Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim SM,Kim JY,Lee S,Park JH

    更新日期:2010-01-04 00:00:00

  • Thiolation of preformed liposomes with iminothiolane.

    abstract::Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5'-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lasch J,Niedermann G,Bogdanov AA,Torchilin VP

    更新日期:1987-04-06 00:00:00

  • A leucine-rich repeat peptide derived from the Drosophila Toll receptor forms extended filaments with a beta-sheet structure.

    abstract::Leucine-rich repeats (LRRs) are 22-28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide deri...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gay NJ,Packman LC,Weldon MA,Barna JC

    更新日期:1991-10-07 00:00:00

  • Functional argument for the existence of an avian nitric oxide synthase in muscle mitochondria: effect of cold acclimation.

    abstract::We report the first evidence of a mitochondrial NO synthase (mtNOS) in bird skeletal muscle. In vitro, mtNOS activity stimulated by L-arginine reduced intermyofibrillar mitochondrial oxygen uptake and ATP synthesis rates, stimulated endogenous H(2)O(2) generation, but had no effect on oxidative phosphorylation efficie...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rey B,Roussel D,Teulier L,Eyenga P,Degletagne C,Belouze M,Duchamp C

    更新日期:2011-01-03 00:00:00

  • Islet-activating protein, pertussis toxin, inhibits Ca2+-induced and guanine nucleotide-dependent releases of histamine and arachidonic acid from rat mast cells.

    abstract::Islet-activating protein (IAP) suppressed Ca2+-induced histamine release and phospholipase A2 activation in Gpp(NH)p-loaded mast cells. Since the guanine nucleotide-binding protein involved in adenylate cyclase inhibition is known to lose its function upon being ADP-ribosylated by IAP, the nucleotide-binding protein i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakamura T,Ui M

    更新日期:1984-08-06 00:00:00

  • Biosynthetic lipid-tagging of antibodies.

    abstract::Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain FV fragments fused to lipoprotein signal peptide and N-term...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Keinänen K,Laukkanen ML

    更新日期:1994-06-06 00:00:00

  • Synthesis and absolute configuration of optically pure enantiomers of a kappa-opioid receptor selective agonist.

    abstract::The enantiomers of U50,488, ligands highly selective for kappa-opioid receptors, have been prepared by a refined procedure and their optical purity demonstrated. The absolute configuration of (+)-trans-2-pyrrolidinyl-N-methylcyclohexylamine, a chemically versatile intermediate for synthesis of analogs of kappa-opioid ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: DeCosta B,George C,Rothman RB,Jacobson AE,Rice KC

    更新日期:1987-11-02 00:00:00

  • FHA domain boundaries of the dun1p and rad53p cell cycle checkpoint kinases.

    abstract::Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

    更新日期:2000-04-14 00:00:00

  • Urmylation and tRNA thiolation functions of ubiquitin-like Uba4·Urm1 systems are conserved from yeast to man.

    abstract::The ubiquitin-like protein Urm1 from budding yeast and its E1-like activator Uba4 have dual roles in protein urmylation and tRNA thiolation pathways. To study whether these are conserved among eukaryotes, we used gene shuffles to replace the yeast proteins by their human counterparts, hURM1 and hUBA4/MOCS3. As judged ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jüdes A,Ebert F,Bär C,Thüring KL,Harrer A,Klassen R,Helm M,Stark MJ,Schaffrath R

    更新日期:2015-04-02 00:00:00

  • Folding kinetics of the lipoic acid-bearing domain of human mitochondrial branched chain alpha-ketoacid dehydrogenase complex.

    abstract::A reversible two-step (native state<-->denatured state) folding mechanism based on equilibrium and stopped flow experiments is proposed for urea denaturation of the lipoyl-bearing domain (hbLBD) of human mitochondrial branched chain alpha-ketoacid dehydrogenase (BCKD) complex. The results from this circular dichroism ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Naik MT,Chang YC,Huang TH

    更新日期:2002-10-23 00:00:00

  • Cl- -dependent photovoltage responses of bacteriorhodopsin: comparison of the D85T and D85S mutants and wild-type acid purple form.

    abstract::Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kalaidzidis IV,Kaulen AD

    更新日期:1997-12-01 00:00:00

  • Atomic force microscopy of DNA molecules.

    abstract::DNA-cytochrome c complexes adsorbed on carbon-coated mica surfaces were directly imaged by atomic force microscopy in air using commercially available cantilevers, with a routine resolution of 6 nm. Images of M13 phage DNA and M13-DNA polymerase complex are also shown. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yang J,Takeyasu K,Shao Z

    更新日期:1992-04-20 00:00:00

  • The phosphoinositide 3-kinase/Akt pathway is activated by daunorubicin in human acute myeloid leukemia cell lines.

    abstract::Daunorubicin induces apoptosis in myeloid leukemia cells by activation of neutral sphingomyelinase and ceramide generation occurring 4-10 min after daunorubicin addition. We show here that daunorubicin is able to increase the phosphoinositide 3-kinase activity and enhance intracellular phosphoinositide 3-kinase lipid ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Plo I,Bettaïeb A,Payrastre B,Mansat-De Mas V,Bordier C,Rousse A,Kowalski-Chauvel A,Laurent G,Lautier D

    更新日期:1999-06-11 00:00:00

  • Analysis of gene expression in rose petals using expressed sequence tags.

    abstract::Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Channelière S,Rivière S,Scalliet G,Szecsi J,Jullien F,Dolle C,Vergne P,Dumas C,Bendahmane M,Hugueney P,Cock JM

    更新日期:2002-03-27 00:00:00

  • Newly translated proteins are substrates for ubiquitin, ISG15, and FAT10.

    abstract::The ubiquitin-like modifier, FAT10, is involved in proteasomal degradation and antigen processing. As ubiquitin and the ubiquitin-like modifier, ISG15, cotranslationally modify proteins, we investigated whether FAT10 could also be conjugated to newly synthesized proteins. Indeed, we found that nascent proteins are mod...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Spinnenhirn V,Bitzer A,Aichem A,Groettrup M

    更新日期:2017-01-01 00:00:00

  • Characterization of the yeast BMH1 gene encoding a putative protein homologous to mammalian protein kinase II activators and protein kinase C inhibitors.

    abstract::We describe the identification and characterization of the BMH1 gene from the yeast Saccharomyces cerevisiae. The gene encodes a putative protein of 292 amino acids which is more than 50% identical with the bovine brain 14-3-3 protein and proteins isolated from sheep brain which are strong inhibitors of protein kinase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van Heusden GP,Wenzel TJ,Lagendijk EL,de Steensma HY,van den Berg JA

    更新日期:1992-05-11 00:00:00

  • Observation of the pre-steady state process in thermolysin catalysis with a fluorescent displacement probe at low pH.

    abstract::The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kunugi S,Yokoyama M,Sakamoto A

    更新日期:1995-04-03 00:00:00

  • Hyperreactivity of adenines and conformational flexibility of a translational repression site.

    abstract::We have used a diethylpyrocarbonate (DEPC) modification [(1976) Prog. Nucl. Acids Res. 16, 189-262] to probe the accessibility of adenines essential for coat protein binding in the MS2 translational operator [(1983) Biochemistry 22, 2601-2610, 2610-2615, 4723-4730; (1987) Biochemistry 26, 1563-1568]. The essential ade...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Talbot SJ,Medina G,Fishwick CW,Haneef I,Stockley PG

    更新日期:1991-05-20 00:00:00

  • Increased cellular cholesterol efflux in glycogen storage disease type Ia mice: a potential mechanism that protects against premature atherosclerosis.

    abstract::Glycogen storage disease type Ia (GSD-Ia) patients manifest a pro-atherogenic lipid profile but are not at elevated risk for developing atherosclerosis. Serum phospholipid, which correlates positively with the scavenger receptor class B type I (SR-BI)-mediated cholesterol efflux, and apolipoprotein A-IV and E, accepto...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nguyen AD,Pan CJ,Shieh JJ,Chou JY

    更新日期:2005-08-29 00:00:00

  • Expression of a human liver cDNA encoding a UDP-glucuronosyltransferase catalysing the glucuronidation of hyodeoxycholic acid in cell culture.

    abstract::A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cel...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fournel-Gigleux S,Jackson MR,Wooster R,Burchell B

    更新日期:1989-01-30 00:00:00

  • Integrin-mediated stimulation of monocyte chemotactic protein-1 expression.

    abstract::We investigated whether activation of integrin receptors could modulate the expression of monocyte chemotactic protein-1 (MCP-1) in human hepatic stellate cells (HSC), mesenchymal cells responsible for extracellular matrix synthesis within the liver. When compared to non-adherent cells, HSC plated on collagen types I ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marra F,Pastacaldi S,Romanelli RG,Pinzani M,Ticali P,Carloni V,Laffi G,Gentilini P

    更新日期:1997-09-08 00:00:00

  • Quantitative study of the binding of cysteine proteinases to basement membranes.

    abstract::Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for Kd determination by competition experiments. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guinec N,Dalet-Fumeron V,Pagano M

    更新日期:1992-08-24 00:00:00

  • Protein kinase C during differentiation of human promyelocytic leukemia cell line, HL-60.

    abstract::Protein kinase C (PKC) from human promyelocytic leukemia HL-60 cells can be resolved into three fractions (peak, a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having beta (beta I and beta II)-sequence and type III having alpha-sequence, respe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hashimoto K,Kishimoto A,Aihara H,Yasuda I,Mikawa K,Nishizuka Y

    更新日期:1990-04-09 00:00:00