Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte.

Abstract:

:The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Tsuji M,Funahashi S,Takigawa M,Seiki M,Fujii K,Yoshida T

doi

10.1016/0014-5793(96)00118-4

subject

Has Abstract

pub_date

1996-03-04 00:00:00

pages

222-6

issue

3

eissn

0014-5793

issn

1873-3468

pii

0014579396001184

journal_volume

381

pub_type

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