Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte.


:The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.




FEBS letters


Tsuji M,Funahashi S,Takigawa M,Seiki M,Fujii K,Yoshida T




Has Abstract


1996-03-04 00:00:00














  • Sequence-specific [1H]NMR resonance assignments and secondary structure identification for 1- and 2-zinc finger constructs from SW15. A hydrophobic core involving four invariant residues.

    abstract::Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SW15 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Neuhaus D,Nakaseko Y,Nagai K,Klug A

    更新日期:1990-03-26 00:00:00

  • Identification of a functional site on the type I TGF-beta receptor by mutational analysis of its ectodomain.

    abstract::Six charged amino acid residues located in the ectodomain of the full-length type I transforming growth factor (TGF)-beta receptor were individually mutated to alanine. Mutation of residues D47, D98, K102 and E104 resulted in functionally impaired receptors as demonstrated by a marked decrease in ligand-dependent sign...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guimond A,Sulea T,Zwaagstra JC,Ekiel I,O'Connor-McCourt MD

    更新日期:2002-02-27 00:00:00

  • WWamide-1, -2 and -3: novel neuromodulatory peptides isolated from ganglia of the African giant snail, Achatina fulica.

    abstract::Three novel neuropeptides, isolated from ganglia of the African giant snail, Achatina fulica, were named WWamide-1, -2 and -3. These substances were biologically active heptapeptide amides with a Trp residue at both the N- and C-termini. WWamide-1, which displayed an inhibitory activity on a central neuron of the snai...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Minakata H,Ikeda T,Muneoka Y,Kobayashi M,Nomoto K

    更新日期:1993-05-24 00:00:00

  • Behavior of a photoactivatable analog of cholesterol, 6-photocholesterol, in model membranes.

    abstract::6-Photocholesterol, a new photoactivatable analog of cholesterol in which a diazirine functionality replaces the 5,6-double bond in the steroid nucleus, was used recently to identify cholesterol-binding proteins in neuroendocrine cells [Thiele, C., Hannah, M.J., Farenholz, F. and Huttner, W.B. (2000) Nat. Cell Biol. 2...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mintzer EA,Waarts BL,Wilschut J,Bittman R

    更新日期:2002-01-16 00:00:00

  • Flexible docking of an amyloid-forming peptide from beta(2)-microglobulin.

    abstract::Using an all-atom, molecular dynamics-based, flexible docking method, the tertiary and quaternary structures of protofilaments of the "K3" fragment from beta(2)-microglobulin (residues Ser20-Lys41) were predicted at low pH in a continuous mixture of water and 2,2,2-trifluoroethanol (TFE). Tetramers with energies very ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Standley DM,Yonezawa Y,Goto Y,Nakamura H

    更新日期:2006-11-13 00:00:00

  • Discrimination between the activity of protein kinase CK2 holoenzyme and its catalytic subunits.

    abstract::The acronym CK2 denotes a highly pleiotropic Ser/Thr protein kinase whose over-expression correlates with neoplastic growth. A vexed question about the enigmatic regulation of CK2 concerns the actual existence in living cells of the catalytic (alpha and/or alpha') and regulatory beta-subunits of CK2 not assembled into...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Salvi M,Sarno S,Marin O,Meggio F,Itarte E,Pinna LA

    更新日期:2006-07-10 00:00:00

  • How often does the myristoylated N-terminal latch of c-Abl come off?

    abstract::The myristoylated N-terminal latching to the C-terminal lobe of c-Abl was recently demonstrated to be an important regulatory element for the kinase, playing a role similar to that of the tyrosine-phosphorylated C-terminal tail of c-Src. A potential mechanism for activating c-Abl is the dissociation of the myristoylat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhou HX

    更新日期:2003-09-25 00:00:00

  • The activity of cAMP-phosphodiesterase 4D7 (PDE4D7) is regulated by protein kinase A-dependent phosphorylation within its unique N-terminus.

    abstract::The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Byrne AM,Elliott C,Hoffmann R,Baillie GS

    更新日期:2015-03-12 00:00:00

  • Investigating the mechanism of ADP-forming acetyl-CoA synthetase from the protozoan parasite Entamoeba histolytica.

    abstract::ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jones CP,Khan K,Ingram-Smith C

    更新日期:2017-02-01 00:00:00

  • The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter.

    abstract::The cDNA coding for the inducible H+/hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose-induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sauer N,Tanner W

    更新日期:1989-12-18 00:00:00

  • Molecular cloning of a novel mouse aspartic protease-like protein that is expressed abundantly in the kidney.

    abstract::By use of the signal sequence trap method, we isolated a cDNA encoding a novel aspartic protease-like protein from the mouse kidney, and termed it 'kidney-derived aspartic protease-like protein (KAP).' The protein, a 419-amino-acid polypeptide with a 16-amino-acid signal sequence, had 47% identity with mouse cathepsin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mori K,Ogawa Y,Tamura N,Ebihara K,Aoki T,Muro S,Ozaki S,Tanaka I,Tashiro K,Nakao K

    更新日期:1997-01-20 00:00:00

  • Enhanced oxidizability of ubiquinol and alpha-tocopherol during lovastatin treatment.

    abstract::A double-blinded, placebo-controlled cross-over trial was carried out with 27 hypercholesterolemic men with coronary heart disease. During the 6-week treatment period lovastatin (60 mg/day) decreased fasting serum LDL cholesterol by 45%, LDL phosphorus by 38% and apoB by 33%. Ubiquinol content diminished by 13% as mea...

    journal_title:FEBS letters

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Palomäki A,Malminiemi K,Metsä-Ketelä T

    更新日期:1997-06-30 00:00:00

  • FXRE can function as an LXRE in the promoter of human ileal bile acid-binding protein (I-BABP) gene.

    abstract::Ileal bile acid-binding protein (I-BABP) is a 14 kDa cytosolic protein which binds bile acids with a high affinity. It is thought to be implicated in the enterohepatic circulation of bile acids and, hence, in cholesterol homeostasis. Using a combination of in vivo and in vitro experiments, we have recently shown that ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Landrier JF,Grober J,Demydchuk J,Besnard P

    更新日期:2003-10-23 00:00:00

  • Adiponectin receptor binding proteins--recent advances in elucidating adiponectin signalling pathways.

    abstract::Adiponectin whose systemic levels are reduced in obesity-related diseases ameliorates insulin sensitivity and regulates biological processes like apoptosis, proliferation, migration and inflammation. Adiponectin binds to adiponectin receptors, AdipoR1 and AdipoR2, which are ubiquitously expressed. Clathrin-dependent e...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Buechler C,Wanninger J,Neumeier M

    更新日期:2010-10-22 00:00:00

  • Receptor phage. Display of functional domains of the human high affinity IgE receptor on the M13 phage surface.

    abstract::In this paper we demonstrate that phage display technology is a suitable system for studying the interaction between the high-affinity receptor for IgE (Fc epsilon RI) and IgE. The alpha subunit extracellular domains of the human receptor were expressed on the surface of filamentous phage M13 fused to the carboxyl-ter...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scarselli E,Esposito G,Traboni C

    更新日期:1993-08-23 00:00:00

  • Metaphase chromosomes from mammalian cells stimulate fusion of artificial phospholipid vesicles.

    abstract::Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Balakireva LA,Belyaev ND,Budker VG,Kiseleva EV

    更新日期:1991-06-24 00:00:00

  • The C-A mismatch base pair and the single-strand terminus in the E. coli initiator tRNA(fMet) acceptor stem adopt unusual conformations.

    abstract::Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zuleeg T,Vogtherr M,Schübel H,Limmer S

    更新日期:2000-04-28 00:00:00

  • An isoform of the human calcitonin receptor is expressed in TT cells and in medullary carcinoma of the thyroid.

    abstract::We amplified, using the polymerase chain reaction and calcitonin receptor (CTR) specific primers, RNA extracted from medullary thyroid carcinoma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in both cases, an ethidium bromide-stained b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Frendo JL,Pichaud F,Mourroux RD,Bouizar Z,Segond N,Moukhtar MS,Jullienne A

    更新日期:1994-04-04 00:00:00

  • High activity of inclusion bodies formed in Escherichia coli overproducing Clostridium thermocellum endoglucanase D.

    abstract::The formation of cytoplasmic inclusion bodies by Escherichia coli overproducing Clostridium thermocellum endoglucanase D (EGD) was investigated. EGD was found in inclusion bodies as a 68 kDa form, whereas the size of the cytoplasmic form was 65 kDa. Upon solubilization with urea followed by dialysis, the 68 kDa form w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tokatlidis K,Dhurjati P,Millet J,Béguin P,Aubert JP

    更新日期:1991-04-22 00:00:00

  • Caenorhabditis elegans small heat-shock proteins Hsp12.2 and Hsp12.3 form tetramers and have no chaperone-like activity.

    abstract::Four 12.2-12.6 kDa small heat-shock proteins (sHSPs) of Caenorhabditis elegans are the smallest known members of the sHSP family. They essentially comprise the characteristic C-terminal 'alpha-crystallin domain' of the sHSPs, having a very short N-terminal region, and lacking a C-terminal tail. Recombinant Hsp12.2 and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kokke BP,Leroux MR,Candido EP,Boelens WC,de Jong WW

    更新日期:1998-08-21 00:00:00

  • A 5 S rRNA-like secondary structure in the 7 SL RNA may define a ribosomal binding site of the signal recognition particle.

    abstract::A new secondary structure model for parts of the 7 SL RNA is proposed which indicates for a stretch of at least 40 bases a strong structural homology to the ribosomal protein L5 binding site of eukaryotic 5 S rRNA. It is suggested that the 5 S rRNA-like structural part of 7 SL RNA mediates binding of the signal recogn...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boehm S

    更新日期:1987-02-09 00:00:00

  • Determination and cellular localization of adenylyl cyclase isozymes expressed in embryonic chick heart.

    abstract::Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yu HJ,Unnerstall JR,Green RD

    更新日期:1995-10-23 00:00:00

  • Inhibition of Moloney murine leukemia virus replication by tyrphostins, tyrosine kinase inhibitors.

    abstract::We have previously shown that topoisomerase I (topo I) antagonist inhibited retrovirus replication. Since tyrphostins, synthetic compounds and protein tyrosine kinases (PTKs) blockers, inhibited topo I activity (manuscript in preparation) we examined their ability to inhibit Moloney murine leukemia virus (Mo-MuLV) rep...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Esther A,Iftach S,Esther P

    更新日期:1994-03-14 00:00:00

  • The nucleocapsid protein of SARS coronavirus has a high binding affinity to the human cellular heterogeneous nuclear ribonucleoprotein A1.

    abstract::The nucleocapsid (N) protein of SARS coronavirus (SARS_CoV) is a major structural component of virions, which appears to be a multifunctional protein involved in viral RNA replication and translation. Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is related to the pre-mRNA splicing in the nucleus and translati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Luo H,Chen Q,Chen J,Chen K,Shen X,Jiang H

    更新日期:2005-05-09 00:00:00

  • alpha-Allenyl putrescine, an enzyme-activated irreversible inhibitor of bacterial and mammalian ornithine decarboxylases.

    abstract::alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Danzin C,Casara P

    更新日期:1984-09-03 00:00:00

  • Diadenosine 5',5"-P1,P5-pentaphosphate harbors the properties of a signaling molecule in the heart.

    abstract::Dinucleotide polyphosphates (ApnA) have emerged as signaling molecules in rapidly dividing cells. The presence and role of Ap5A in the heart remain unknown. Here, we report that the myocardium contains abundant amounts of diadenosine 5',5"-P1,P5-pentaphosphate (Ap5A), a member of the ApnA family. Ischemia induced 10-f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jovanovic A,Jovanovic S,Mays DC,Lipsky JJ,Terzic A

    更新日期:1998-02-27 00:00:00

  • Kaempferol is an estrogen-related receptor alpha and gamma inverse agonist.

    abstract::Kaempferol is a dietary flavonoid that is thought to function as a selective estrogen receptor modulator. In this study, we established that kaempferol also functions as an inverse agonist for estrogen-related receptors alpha and gamma (ERRalpha and ERRgamma). We demonstrated that kaempferol binds to ERRalpha and ERRg...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang J,Fang F,Huang Z,Wang Y,Wong C

    更新日期:2009-02-18 00:00:00

  • Kinetic properties of cobalt--iron hybrid hemoglobins.

    abstract::The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oertle M,Winterhalter KH,Di Iorio EE

    更新日期:1983-03-07 00:00:00

  • Conversion of the sodium channel activator aconitine into a potent alpha 7-selective nicotinic ligand.

    abstract::Methyllycaconitine (MLA) is a competitive antagonist of nicotinic acetylcholine receptors, with a remarkable preference for neuronal [125I]alpha Bgt binding sites. We have begun to investigate the structural basis of its potency and subtype selectivity. MLA is a substituted norditerpenoid alkaloid linked to a 2-(methy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hardick DJ,Cooper G,Scott-Ward T,Blagbrough IS,Potter BV,Wonnacott S

    更新日期:1995-05-22 00:00:00

  • Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

    abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

    更新日期:2001-11-23 00:00:00