Protonated triplex DNA in E. coli cells as detected by chemical probing.

abstract：:During steady-state, the Pi released in the medium is derived from glucose-6-phosphate which continuously regenerates the ATP hydrolyzed. A membrane potential (delta psi) can be built up in submitochondrial particles using glucose-6-phosphate and hexokinase as an ATP-regenerating system. The energy derived from the me...

journal_title：FEBS letters

authors： de Meis L,Grieco MA,Galina A

更新日期：1992-08-17 00:00:00

abstract：:We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (DeltaPsi). To clarify the mechanism underlying the elevation of DeltaPsi, modular kinetic analysis was applied to oxidative ...

journal_title：FEBS letters

authors： Kikusato M,Ramsey JJ,Amo T,Toyomizu M

更新日期：2010-07-16 00:00:00

abstract：:The Escherichia coli twin-arginine translocation (Tat) system serves to export fully folded protein substrates across the bacterial cytoplasmic membrane. Respiratory [NiFe] hydrogenases are synthesised as precursors with twin-arginine signal peptides and transported as large, cofactor-containing, multi-subunit complex...

journal_title：FEBS letters

authors： Dubini A,Sargent F

更新日期：2003-08-14 00:00:00

abstract：:Cyanomorpholinoadriamycin (1 microM) was reacted in a transcription buffer with DNA of an initiated transcription complex. Subsequent elongation of the initiated complex revealed permanent transcriptional blockages at 16 sites after only 5 min of drug-DNA reaction time. The most dominant sites were immediately prior t...

journal_title：FEBS letters

authors： Cullinane C,Phillips DR

更新日期：1991-11-18 00:00:00

abstract：:Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell l...

journal_title：FEBS letters

authors： Tanaka N,Watanabe J,Kitamura T,Yamada Y,Kanegasaki S,Nakata K

更新日期：1999-01-15 00:00:00

abstract：:A photoactive reaction center complex was isolated from a thermophilic green sulfur bacterium, Chlorobium tepidum under anaerobic conditions. The electron transfer occurred from heme c to the photo-oxidized reaction center chlorophyll, P840+, with a half time (t1/2) of 110 or 340 microseconds at 24 or 12 degrees C, re...

journal_title：FEBS letters

authors： Oh-oka H,Kamei S,Matsubara H,Iwaki M,Itoh S

更新日期：1995-05-22 00:00:00

abstract：:Excessive reactive oxygen species (ROS) play a key role in the pathogenesis of diabetic nephropathy. The thioredoxin (TRX) system, a major thiol antioxidant system, regulates the reduction of intracellular ROS. Here we show that high glucose (HG) inhibits TRX ROS-scavenging function through p38 mitogen-activated prote...

journal_title：FEBS letters

authors： Ren Y,Shi Y,Wang Y,Li Y,Wu S,Li H,Zhang Y,Duan H

更新日期：2010-08-04 00:00:00

abstract：:CHO SSF3 cells grow as a suspension culture in unmodified commercial medium with only low-molecular weight ingredients. Continuous serum-free culture unexpectedly induced expression of a low dihydrofolate reductase activity in the originally dhfr- CHO cells. Nevertheless, it was possible with methotrexate to induce am...

journal_title：FEBS letters

authors： Gandor C,Leist C,Fiechter A,Asselbergs FA

更新日期：1995-12-27 00:00:00

abstract：:Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...

journal_title：FEBS letters

authors： Manjithaya R,Nazarko TY,Farré JC,Subramani S

更新日期：2010-04-02 00:00:00

abstract：:We analysed the biochemical properties of insulin receptors of a Type A insulin resistant patient with a single heterozygous point mutation substituting Gln for Arg1174. Insulin binding capacity and affinity to Epstein-Barr virus transformed lymphocytes was normal. Quantitative analysis of autophosphorylation and subs...

journal_title：FEBS letters

authors： Moritz W,Froesch ER,Böni-Schnetzler M

更新日期：1994-09-05 00:00:00

abstract：:Members of the heat shock protein 70 (Hsp70) family are found in most of the compartments of eukaryotic cells where they play essential roles in protein metabolism. In yeast mitochondria, two Hsp70 proteins are known: Ssc1 and Ssq1. We identified Ecm10 as a third Hsp70 protein in the mitochondrial matrix. Ecm10 shares...

journal_title：FEBS letters

authors： Baumann F,Milisav I,Neupert W,Herrmann JM

更新日期：2000-12-29 00:00:00

abstract：:There is considerable epidemiological evidence that tea drinking lowers the risk of heart disease. However, the mechanism by which tea can be protective is unknown. Hamsters were fed a normal or high cholesterol diet for 2 weeks and drank green or black tea ad libitum. The plasma lipid profile was significantly improv...

journal_title：FEBS letters

authors： Vinson JA,Dabbagh YA

更新日期：1998-08-14 00:00:00

abstract：:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

journal_title：FEBS letters

authors： Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

更新日期：1994-08-08 00:00:00

abstract：:Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production afte...

journal_title：FEBS letters

authors： Nabben M,Hoeks J,Moonen-Kornips E,van Beurden D,Briedé JJ,Hesselink MK,Glatz JF,Schrauwen P

更新日期：2011-12-15 00:00:00

abstract：:Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5' rapid amplification of cDNA ends (RACE) shows that nearly all uncapped ends correspond to...

journal_title：FEBS letters

authors： Berger MR,Alvarado R,Kiss DL

更新日期：2019-04-01 00:00:00

abstract：:A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partial...

journal_title：FEBS letters

authors： Bennett EP,Hassan H,Hollingsworth MA,Clausen H

更新日期：1999-10-29 00:00:00

abstract：:Two highly contrasted images depict genomes: at first sight, genes appear to be distributed randomly along the chromosome. In contrast, their organisation into operons (or pathogenicity islands) suggests that, at least locally, related functions are in physical proximity. Analysis of the codon usage bias in orthologou...

journal_title：FEBS letters

authors： Rocha EP,Sekowska A,Danchin A

更新日期：2000-06-30 00:00:00

abstract：:The temperature dependence of single-channel conductance and open probability for outer membrane protein A (OmpA) of Escherichia coli were examined in planar lipid bilayers. OmpA formed two interconvertible conductance states, small channels, 36-140 pS, between 15 and 37 degrees C, and large channels, 115-373 pS, betw...

journal_title：FEBS letters

authors： Zakharian E,Reusch RN

更新日期：2003-12-04 00:00:00

abstract：:An efficient method is presented for the production of intact mammalian prion proteins and partial sequences thereof. As an illustration we describe the production of polypeptides comprising residues 23-231, 81-231, 90-231 and 121-231 of the human prion protein (hPrP). Polypeptides were expressed as histidine tail fus...

journal_title：FEBS letters

authors： Zahn R,von Schroetter C,Wüthrich K

更新日期：1997-11-17 00:00:00

abstract：:The sarcoplasmic reticulum Ca(2+)-ATPase loses hydrolytic activity and the ability to be phosphorylated by Pi following incubation with EDC [1-ethyl-3-(3-dimethylaminopropyl)carbodiimide]. 4 nmol of tempamine per mg SR protein can be coupled to either a glu or an asp side chain through the EDC reaction. Mg2+ protects ...

journal_title：FEBS letters

authors： Coan C,Jakobs P,Ji JY,Murphy AJ

更新日期：1993-11-29 00:00:00

abstract：:In this paper we demonstrate that phage display technology is a suitable system for studying the interaction between the high-affinity receptor for IgE (Fc epsilon RI) and IgE. The alpha subunit extracellular domains of the human receptor were expressed on the surface of filamentous phage M13 fused to the carboxyl-ter...

journal_title：FEBS letters

authors： Scarselli E,Esposito G,Traboni C

更新日期：1993-08-23 00:00:00

abstract：:DNA is very stable in its double-stranded form. For many processes of DNA metabolism, such as replication, repair, recombination and transcription, the DNA has to be brought transiently into a single-stranded form. DNA helicases are enzymes capable of melting the hydrogen bonds of base pairs by using the energy of nuc...

journal_title：FEBS letters

authors： Thömmes P,Hübscher U

更新日期：1990-08-01 00:00:00

abstract：:Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...

journal_title：FEBS letters

authors： Song M,Zhu N,Olcese R,Barila B,Toro L,Stefani E

更新日期：1999-11-05 00:00:00

abstract：:Rat brain S-100 (S-100b) and ox brain S-100a0, S-100a and S-100b have been tested for their ability to control the assembly and disassembly of brain microtubule proteins in the presence of either Ca2+ or Zn2+, in vitro. In the presence of Ca2+, single S-100 isoforms have similar, if not identical, effects, i.e. they i...

journal_title：FEBS letters

authors： Donato R,Isobe T,Okuyama T

更新日期：1985-07-01 00:00:00

abstract：:Both HIV infection per se and antiretroviral drugs might contribute to oxidative stress and mitochondrial dysfunctions. In this study we assess zidovudine, stavudine and didanosine on U937 and CEM cell lines. All these drugs induced apoptosis and increased intracellular hydrogen peroxide but not superoxide anions. The...

journal_title：FEBS letters

authors： Ferraresi R,Troiano L,Roat E,Nemes E,Lugli E,Nasi M,Pinti M,Calvani M,Iannuccelli M,Cossarizza A

更新日期：2006-12-11 00:00:00

abstract：:In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 wer...

journal_title：FEBS letters

authors： Kregler O,Schilf R,Lander A,Bannert N,Bogner E

更新日期：2009-04-02 00:00:00

abstract：:The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...

journal_title：FEBS letters

authors： Inohara T,Tarui M,Doi M,Inoue M,Ishida T

更新日期：1993-06-21 00:00:00

abstract：:2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...

journal_title：FEBS letters

authors： Rafael J,Nicholls DG

更新日期：1984-05-07 00:00:00

abstract：:The conserved KTG triad in the class C beta-lactamase from Citrobacter freundii GN346 was examined as to its function by means of site-directed mutagenesis. The following conversions were performed; Lys-315 to arginine, alanine or glutamic acid, Thr-316 to valine, and Gly-317 to alanine, proline or isoleucine. The res...

journal_title：FEBS letters

authors： Tsukamoto K,Nishida N,Tsuruoka M,Sawai T

更新日期：1990-10-01 00:00:00

abstract：:Guluronate and mannuronate oligomers with various degree of polymerization were prepared from polyguluronate (PG) and polymannuronate (PM) with an alginate lyase from a Pseudoalteromonas sp., and their activities to induce cytokine secretion from mouse macrophage cell line RAW264.7 cells were examined. Enzymatically d...

journal_title：FEBS letters

authors： Iwamoto M,Kurachi M,Nakashima T,Kim D,Yamaguchi K,Oda T,Iwamoto Y,Muramatsu T

更新日期：2005-08-15 00:00:00