Abstract:
:A photoactive reaction center complex was isolated from a thermophilic green sulfur bacterium, Chlorobium tepidum under anaerobic conditions. The electron transfer occurred from heme c to the photo-oxidized reaction center chlorophyll, P840+, with a half time (t1/2) of 110 or 340 microseconds at 24 or 12 degrees C, respectively. Optical measurements under multiflash excitations indicated that two hemes function as the immediate electron donors to P840+. SDS-PAGE analysis of the RC complex in combination with the N-terminal amino acid sequence analyses revealed five subunit bands; a core protein (65 kDa), the light harvesting bacteriochlorophyll alpha protein (41 kDa), a protein with 2[4Fe-4S] clusters (31 kDa), monoheme cytochrome c (22 kDa), and a 18-kDa protein whose function is unknown. The reaction center complex, thus, contains two molecules of cytochrome c per P840.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Oh-oka H,Kamei S,Matsubara H,Iwaki M,Itoh Sdoi
10.1016/0014-5793(95)00433-asubject
Has Abstractpub_date
1995-05-22 00:00:00pages
30-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(95)00433-Ajournal_volume
365pub_type
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