Abstract:
:hTrp3 is a human homologue of the Drosophila gene responsible for a transient receptor potential (trp) mutation. When stably expressed in HEK293 cells, hTrp3 formed ion channels that were active under resting conditions but could be further stimulated by carbachol or ATP via endogenous muscarinic or purinergic receptors, respectively. Agonist evoked currents reversed polarity near 0 mV in physiological ionic conditions and were associated with a significant increase in the current variance. These results suggest the involvement of a non-selective cation channel with relatively large unitary amplitude. Consistent with this, resolved unitary events had a conductance of approximately 60 pS in the negative voltage range and an extrapolated reversal potential near 0 mV.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hurst RS,Zhu X,Boulay G,Birnbaumer L,Stefani Edoi
10.1016/s0014-5793(98)00035-0subject
Has Abstractpub_date
1998-02-06 00:00:00pages
333-8issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00035-0journal_volume
422pub_type
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