Spleen protein tyrosine kinases TPK-IIB and CSK display different immunoreactivity and opposite specificities toward c-src-derived peptides.

abstract：:We have used fluorescence correlation spectroscopy to analyze the interaction of GTP-tubulin with rhodamine-labeled RB3, a neural protein of the stathmin family, and to determine the kinetic pathway of the association process. RB3 displayed slow association-dissociation kinetics with tubulin depending on the square of...

journal_title：FEBS letters

authors： Krouglova T,Amayed P,Engelborghs Y,Carlier MF

更新日期：2003-07-10 00:00:00

abstract：:It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu...

journal_title：FEBS letters

authors： Hartmann HJ,Morpurgo L,Desideri A,Rotilio G,Weser U

更新日期：1983-02-07 00:00:00

abstract：:Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope...

journal_title：FEBS letters

authors： Guihard G,Proteau S,Payet MD,Escande D,Rousseau E

更新日期：2000-07-07 00:00:00

abstract：:Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...

journal_title：FEBS letters

authors： Takahashi M,Tezuka T,Kakegawa H,Katunuma N

更新日期：1994-03-07 00:00:00

abstract：:Neoplastic cells metabolize higher amounts of glucose relative to normal cells in order to cover increased energetic and anabolic needs. Inhibition of the glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) diminishes cancer cell proliferation and tumour growth in animals. In this work, w...

journal_title：FEBS letters

authors： Simon-Molas H,Calvo-Vidal MN,Castaño E,Rodríguez-García A,Navarro-Sabaté À,Bartrons R,Manzano A

更新日期：2016-09-01 00:00:00

abstract：:Under destabilising conditions both heat and cold denaturation of yeast phosphoglycerate kinase (PGK) can be observed. According to previous interpretation of experimental data and theoretical calculations, the C-terminal domain should be more stable than the N-terminal domain at all temperatures. We report on thermal...

journal_title：FEBS letters

authors： Gast K,Damaschun G,Desmadril M,Minard P,Müller-Frohne M,Pfeil W,Zirwer D

更新日期：1995-01-30 00:00:00

abstract：:Magnesium (Mg(2+)) transport across membranes plays an essential role in cellular growth and survival. TRPM7 is the unique fusion of a Mg(2+) permeable pore with an active cytosolic kinase domain, and is considered a master regulator of cellular Mg(2+) homeostasis. We previously found that the genetic deletion of TRPM...

journal_title：FEBS letters

authors： Deason-Towne F,Perraud AL,Schmitz C

更新日期：2011-07-21 00:00:00

abstract：:The tailspike protein (TSP) of bacteriophage P22 is a homotrimeric multifunctional protein responsible for cell attachment and hydrolysis of the Salmonella typhimurium host cell receptor. Despite the folding of TSP involves the formation of thermolabile intermediates, the mature protein is extremely resistant to heat ...

journal_title：FEBS letters

authors： Carbonell X,Villaverde A

更新日期：1998-08-07 00:00:00

abstract：:The changes in phosphoproteins purified with the affinity peptide p9CKShs1 were analyzed from extracts of regenerating rat livers in order to define some G1 and G1/S regulations characteristic of mature hepatocytes stimulated to proliferate. We observed a 47 kDa phosphoprotein that occurred first at the end of G1 befo...

journal_title：FEBS letters

authors： Bilodeau M,Talarmin H,Ilyin G,Rescan C,Glaise D,Cariou S,Loyer P,Guguen-Guillouzo C,Baffet G

更新日期：1999-06-11 00:00:00

abstract：:The binding of phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)) to profilin at a region distinct from the actin interaction surface is demonstrated by experiments with covalently cross-linked profilin:beta-actin. The result is in agreement with observations made with several mutant profilins and provides strong ev...

journal_title：FEBS letters

authors： Skare P,Karlsson R

更新日期：2002-07-03 00:00:00

abstract：:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

journal_title：FEBS letters

authors： Battu S,Beneytout JL,Pairet M,Rigaud M

更新日期：1998-10-16 00:00:00

abstract：:Oligonucleotide-peptide conjugates have several applications, including their potential use as improved antisense agents for interfering with the RNA function within cells. In order to provide robust and generally applicable conjugation chemistry, we developed a novel approach of fragment coupling of pre-synthesized p...

journal_title：FEBS letters

authors： Zubin EM,Romanova EA,Volkov EM,Tashlitsky VN,Korshunova GA,Shabarova ZA,Oretskaya TS

更新日期：1999-07-30 00:00:00

abstract：:The bacterial cell wall muramyl dipeptides MDP and glucosaminyl-MDP (GMDP) are powerful immunostimulators but their binding target remains controversial. We previously reported expression cloning of GMDP-binding polypeptides and identification of Y-box protein 1 (YB-1) as their sole target. Here we show specific bindi...

journal_title：FEBS letters

authors： Laman AG,Lathe R,Savinov GV,Shepelyakovskaya AO,Boziev KhM,Baidakova LK,Chulin AN,Brovko FA,Svirshchevskaya EV,Kotelevtsev Y,Eliseeva IA,Guryanov SG,Lyabin DN,Ovchinnikov LP,Ivanov VT

更新日期：2015-07-08 00:00:00

abstract：:Neuritic plaques are the key pathological feature of Alzheimer's disease, and amyloid beta (Abeta) peptides are major component of these plaques. In this study, we demonstrated the influence of aluminum (Al) on the Abeta peptide degradation by cathepsin D. Al did not directly affect the cathepsin D activity using smal...

journal_title：FEBS letters

authors： Sakamoto T,Saito H,Ishii K,Takahashi H,Tanabe S,Ogasawara Y

更新日期：2006-12-11 00:00:00

abstract：:Annexin-I is a calcium dependent phospholipid binding and phospholipase A2 (PLA2) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA2 inhibition by annexin-I in studies with 14 to 18 kDa PLA2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA2 from ...

journal_title：FEBS letters

authors： Kim KM,Kim DK,Park YM,Kim CK,Na DS

更新日期：1994-05-02 00:00:00

abstract：:The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine...

journal_title：FEBS letters

authors： van Heyningen S

更新日期：1983-11-28 00:00:00

abstract：:Photo-oxidized low-density lipoprotein is cytotoxic to bovine aortic endothelial cells in a concentration-dependent manner. Total cell killing occurs at a concentration of 600 mumol/l lipid hydroperoxide (LOOH). Selenium deficiency enhances the toxicity of LOOH such that 300 mumol/l LOOH is cytotoxic. This toxicity is...

journal_title：FEBS letters

authors： Struck AT,Hogg N,Thomas JP,Kalyanaraman B

更新日期：1995-03-20 00:00:00

abstract：:STAT3, a transcription factor involved in various physiological and pathological processes, is also present in mitochondria. Mitochondrial STAT3 regulates complex I activity and reactive oxygen species (ROS) production, yet the mechanisms governing its translocation to mitochondria remain poorly understood. In this st...

journal_title：FEBS letters

authors： Mohammed F,Gorla M,Bisoyi V,Tammineni P,Sepuri NBV

更新日期：2020-05-01 00:00:00

abstract：:Oscillations of free intracellular calcium [Ca2+]i were seen in individual Jurkat cells as response to a 50 Hz, 0.15 mT magnetic field (MF). In contrast, a CD45-deficient Jurkat cell line was unable to respond to MF stimulation. The phosphatase activity of CD45 has been implicated to regulate p56lck tyrosine kinase ac...

journal_title：FEBS letters

authors： Lindström E,Berglund A,Mild KH,Lindström P,Lundgren E

更新日期：1995-08-14 00:00:00

abstract：:Apoptosis specific proteins (ASP) are expressed in the cytoplasm of cultured mammalian cells of various lineages following induction of apoptosis. The cDNA encoding ASP has been cloned from a human expression library and has significant homology to the Saccharomyces cerevisiae APG5 gene which is essential for yeast au...

journal_title：FEBS letters

authors： Hammond EM,Brunet CL,Johnson GD,Parkhill J,Milner AE,Brady G,Gregory CD,Grand RJ

更新日期：1998-04-03 00:00:00

abstract：:The present study discusses the efficacy of Aloe emodin-8-O-glycoside (AEG), a plant derived anthroquinone, on alleviating insulin resistance and augmenting glycogen synthesis in L6 myotubes and 3T3L1 adipocytes. Dose-dependent increase in glucose uptake activity (GUA) was observed in both cell lines. Immunoblot analy...

journal_title：FEBS letters

authors： Anand S,Muthusamy VS,Sujatha S,Sangeetha KN,Bharathi Raja R,Sudhagar S,Poornima Devi N,Lakshmi BS

更新日期：2010-07-16 00:00:00

abstract：:The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality ...

journal_title：FEBS letters

authors： Skottman H,Dilber MS,Hovatta O

更新日期：2006-05-22 00:00:00

abstract：:In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 wer...

journal_title：FEBS letters

authors： Kregler O,Schilf R,Lander A,Bannert N,Bogner E

更新日期：2009-04-02 00:00:00

abstract：:We studied the effect of high-fat diet on the expression and activation of the three caveolins in rat skeletal muscle and their association with the insulin signalling cascade. Initial response was characterized by increased signalling through Cav-1 and Cav-3 phosphorylation, suggesting that both participate in an ini...

journal_title：FEBS letters

authors： Gómez-Ruiz A,de Miguel C,Campión J,Martínez JA,Milagro FI

更新日期：2009-10-06 00:00:00

abstract：:The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constan...

journal_title：FEBS letters

authors： Zenkova MA,Fedorova OS,Levina AS,Mamaev SV,Karpova GG

更新日期：1990-08-20 00:00:00

abstract：:PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...

journal_title：FEBS letters

authors： Philipp S,Flockerzi V

更新日期：1997-08-18 00:00:00

abstract：:To search for negative regulatory components of the NF-kappaB activation pathways, we mutagenized Rat-1 fibroblasts and established a stable mutant cell line with a constitutive NF-kappaB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically ...

journal_title：FEBS letters

authors： Chinanonwait N,Miura H,Yamamoto N,Yamaoka S

更新日期：2002-11-20 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:C2cd4c, encoded by a gene belonging to the C2cd4 family, contains a C2 domain conserved across species and is localized to the cytoplasm. To examine the role of C2cd4c in the pancreas, we studied its localization and generated C2cd4c knockout (KO) mice. C2cd4c was expressed in pancreatic endocrine progenitors at early...

journal_title：FEBS letters

authors： Omori H,Ogaki S,Sakano D,Sato M,Umeda K,Takeda N,Nakagata N,Kume S

更新日期：2016-08-01 00:00:00

abstract：:Prolonged incubation of membrane fragments containing homogeneous (Na+ + K+)-ATPase with Mg2+, K+ and VO-3 at 4 degrees C resulted in formation of two-dimensional crystals of this enzyme with unit cell parameters: a = 66 A, b = 118 A, gamma = 108 degrees. The crystals correspond to the two-sided plane group p21. By co...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Demin VV,Barnakov AN,Kuzin AP,Lunev AV,Modyanov NN,Dzhandzhugazyan KN

更新日期：1985-10-07 00:00:00