Abstract:
:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents an original mammalian model to study FLAP-dependent leukotriene synthesis. In FLAP cDNA transfected HT29 cl.19A cells, FLAP expression led to an increase in cyclooxygenase pathway products (mainly PGE2) without an increase in 5-lipoxygenase metabolism. This increase in PGE2 synthesis was associated with a cyclooxygenase-2 upregulation in comparison to untransfected HT29 cl.19A cells. These results suggest a possible interaction between the two major pathways of arachidonic acid metabolism.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Battu S,Beneytout JL,Pairet M,Rigaud Mdoi
10.1016/s0014-5793(98)01196-xsubject
Has Abstractpub_date
1998-10-16 00:00:00pages
49-55issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(98)01196-Xjournal_volume
437pub_type
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