Abstract:
:Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-L-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sengupta R,Billiar TR,Atkins JL,Kagan VE,Stoyanovsky DAdoi
10.1016/j.febslet.2009.10.016subject
Has Abstractpub_date
2009-11-03 00:00:00pages
3525-30issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00787-Xjournal_volume
583pub_type
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