Abstract:
:Rho proteins are down-regulated in vivo by specific GTPase activating proteins (RhoGAP). We have functionally studied three Saccharomyces cerevisiae putative RhoGAP. By first identifying Rho partners with a systematic two-hybrid approach and then using an in vitro assay, we have demonstrated that the Bag7 protein stimulated the GTPase activity of the Rho1 protein, Lrg1p acted on the Cdc42 and Rho2 GTPases and we showed that Rgd2p has a GAP activity on both Cdc42p and Rho5p. In addition, we brought the first evidence for the existence of a sixth functional Rho in yeast, the Cdc42/Rac-like GTPase Rho5.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Roumanie O,Weinachter C,Larrieu I,Crouzet M,Doignon Fdoi
10.1016/s0014-5793(01)02906-4keywords:
subject
Has Abstractpub_date
2001-10-05 00:00:00pages
149-56issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02906-4journal_volume
506pub_type
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