Abstract:
:The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate beta-subunit peptide, two additional proteins with apparent Mr 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of Ch. reinhardtii.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Korolkov SN,Garnovskaya MN,Basov AS,Chunaev AS,Dumler ILdoi
10.1016/0014-5793(90)81251-isubject
Has Abstractpub_date
1990-09-17 00:00:00pages
132-4issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81251-Ijournal_volume
270pub_type
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