Stress-specific aggregation of proteins in the amyloid bodies.

abstract：:By use of the signal sequence trap method, we isolated a cDNA encoding a novel aspartic protease-like protein from the mouse kidney, and termed it 'kidney-derived aspartic protease-like protein (KAP).' The protein, a 419-amino-acid polypeptide with a 16-amino-acid signal sequence, had 47% identity with mouse cathepsin...

journal_title：FEBS letters

authors： Mori K,Ogawa Y,Tamura N,Ebihara K,Aoki T,Muro S,Ozaki S,Tanaka I,Tashiro K,Nakao K

更新日期：1997-01-20 00:00:00

abstract：:Crigler-Najjar (CN) disease is caused by a deficiency of the hepatic enzyme, bilirubin UDP-glucuronosyltransferase (B-UGT). We have found two CN type II patients, who were homozygous for a leucine to arginine transition at position 15 of B-UGT1. This mutation is expected to disrupt the hydrophobic core of the signal p...

journal_title：FEBS letters

authors： Seppen J,Steenken E,Lindhout D,Bosma PJ,Elferink RP

更新日期：1996-07-29 00:00:00

abstract：:Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SW15 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises...

journal_title：FEBS letters

authors： Neuhaus D,Nakaseko Y,Nagai K,Klug A

更新日期：1990-03-26 00:00:00

abstract：:We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

journal_title：FEBS letters

authors： Boukamel R,Freund JN

更新日期：1994-10-10 00:00:00

abstract：:Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypept...

journal_title：FEBS letters

authors： Carver MA,Jones CW

更新日期：1983-05-08 00:00:00

abstract：:The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared througho...

journal_title：FEBS letters

authors： Shahhoseini M,Favaedi R,Baharvand H,Sharma V,Stunnenberg HG

更新日期：2010-11-19 00:00:00

abstract：:The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G4-forming amylase) has been determined. The coding region for the G4-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced m...

journal_title：FEBS letters

authors： Zhou JH,Baba T,Takano T,Kobayashi S,Arai Y

更新日期：1989-09-11 00:00:00

abstract：:The effects of various local anaesthetics (LAs) on ryanodine binding of the sheep brain ryanodine receptor were tested. Tetracaine and dibucaine inhibit the binding with half-maximal inhibition (CI50) of 0.12 mM and 0.7 mM, respectively. Lidocaine and its analog QX-314, on the other hand, stimulate the binding up to 3...

journal_title：FEBS letters

authors： Martin C,Ashley R,Shoshan-Barmatz V

更新日期：1993-08-09 00:00:00

abstract：:Malonyl-CoA-acyl carrier protein transacylase (MCAT) transfers the malonyl group from malonyl-CoA to holo-acyl carrier protein (ACP), and since malonyl-ACP is a key building block for fatty-acid biosynthesis it is considered as a promising antibacterial target. The crystal structures of MCAT from Staphylococcus aureus...

journal_title：FEBS letters

authors： Hong SK,Kim KH,Park JK,Jeong KW,Kim Y,Kim EE

更新日期：2010-03-19 00:00:00

abstract：:Phenylalanine-specific tRNA from yeast was hydrolysed with cobra venom ribonuclease in the double-stranded regions and the fragments isolated. The 'dissected' molecules with nicks in positions 28 and 41 were reconstructed from supplementary fragments and treated with T-4 RNA ligase. A phosphodiester bond between two f...

journal_title：FEBS letters

authors： Boutorin AS,Vassilenko SK,Baklanov MM,Nechaev YS

更新日期：1984-01-02 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:To study Bax-induced release of cytochrome c in vivo, we have expressed a cytochrome c-GFP (green fluorescent protein) fusion in Saccharomyces cerevisiae cells null for the expression of the endogenous cytochrome. We show here that cytochrome c-GFP is efficiently localised to mitochondria and able to function as an el...

journal_title：FEBS letters

authors： Roucou X,Prescott M,Devenish RJ,Nagley P

更新日期：2000-04-14 00:00:00

abstract：:The mechanism of stimulation of hepatic respiration by fatty acids was studied in isolated rat hepatocytes. Stimulation of respiration by fatty acids varied from about 35% to about 105% depending on chain length. The stimulatory effect of octanoate (1 mM) or oleate (0.5 mM) was prevented by oligomycin (2 micrograms/ml...

journal_title：FEBS letters

authors： Plomp PJ,van Roermund CW,Groen AK,Meijer AJ,Tager JM

更新日期：1985-12-02 00:00:00

abstract：:Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin ...

journal_title：FEBS letters

authors： Yagita K,Okamura H

更新日期：2000-01-07 00:00:00

abstract：:We have identified and analyzed a 27-nucleotide sequence (U5 repressive element, designated as U5RE) at the U5 region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) which is required for HTLV-I basal transcriptional repression. The basal promoter strength of constructs that contained del...

journal_title：FEBS letters

authors： Okumura K,Takagi S,Sakaguchi G,Naito K,Minoura-Tada N,Kobayashi H,Mimori T,Hinuma Y,Igarashi H

更新日期：1994-12-12 00:00:00

abstract：:By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A2 (PLA2), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA2 (sPLA2-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells...

journal_title：FEBS letters

authors： Kambe T,Murakami M,Kudo I

更新日期：1999-06-18 00:00:00

abstract：:The amino acid sequence of the eclosion hormone from the tobacco hornworm Manduca sexta has been determined, using less than 500 pmol of protein and microanalytical techniques. The protein contains 62 amino acid residues and has a molecular mass of 6813 Da. The amino-terminal sequence is similar to that of a 13-residu...

journal_title：FEBS letters

authors： Marti T,Takio K,Walsh KA,Terzi G,Truman JW

更新日期：1987-07-27 00:00:00

abstract：:A novel GTP binding protein (G protein) alpha subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (Tc alpha). The orga...

journal_title：FEBS letters

authors： Kubo M,Hirano T,Kakinuma M

更新日期：1991-10-21 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...

journal_title：FEBS letters

authors： Perkins KJ,Davies KE

更新日期：2018-06-01 00:00:00

abstract：:Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...

journal_title：FEBS letters

authors： Meimoun P,Gousset-Dupont A,Lebouteiller B,Ambard-Bretteville F,Besin E,Lelarge C,Mauve C,Hodges M,Vidal J

更新日期：2009-05-19 00:00:00

abstract：:The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimension...

journal_title：FEBS letters

authors： Torda AE,Mabbutt BC,van Gunsteren WF,Norton RS

更新日期：1988-11-07 00:00:00

abstract：:Phosphorylcholine (PC) is a classical ligand of C-reactive protein (CRP), a clinically important acute phase protein. In search of new ligands, CRPs were affinity-purified from several pathological samples, which exhibited distinct molecular variants induced in different diseases. Both glycosylated and non-glycosylate...

journal_title：FEBS letters

authors： Das T,Mandal C,Mandal C

更新日期：2004-10-08 00:00:00

abstract：:The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously d...

journal_title：FEBS letters

authors： Viglino P,Fogolari F,Formisano S,Bortolotti N,Damante G,Di Lauro R,Esposito G

更新日期：1993-12-28 00:00:00

abstract：:The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...

journal_title：FEBS letters

authors： Cenci di Bello I,Dorling P,Fellows L,Winchester B

更新日期：1984-10-15 00:00:00

abstract：:One type of covalent linkages connecting lignin and hemicellulose in plant cell walls is the ester linkage between 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols. An enzyme that could hydrolyze such linkages, named glucuronoyl esterase, occurs in the cellulolytic system of the wood-rotting fungus S...

journal_title：FEBS letters

authors： Li XL,Spániková S,de Vries RP,Biely P

更新日期：2007-08-21 00:00:00

abstract：:We previously isolated, by differential display and 5' RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for tobacco cryptogein Induced), a gene encoding a beta-subunit of proteasome. Here, we report that tc...

journal_title：FEBS letters

authors： Etienne P,Petitot AS,Houot V,Blein JP,Suty L

更新日期：2000-01-28 00:00:00

abstract：:Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solub...

journal_title：FEBS letters

authors： Rich DH,Kawai M,Goodman HL,Engelke J,Suttie JW

更新日期：1983-02-07 00:00:00

abstract：:Dosage compensation involves fine-tuning of gene expression at the level of entire chromosomes. The principles that assure selective targeting of the male X chromosome in Drosophila and the mechanism by which transcription levels are adjusted in a twofold range are still mysterious. We discuss the prevalent models in ...

journal_title：FEBS letters

authors： Straub T,Dahlsveen IK,Becker PB

更新日期：2005-06-13 00:00:00

abstract：:Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 x 35 = 245 residues. The repeat-motifs are likely to be largely alpha-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin ...

journal_title：FEBS letters

authors： Labeit S,Gibson T,Lakey A,Leonard K,Zeviani M,Knight P,Wardale J,Trinick J

更新日期：1991-05-06 00:00:00

abstract：:Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...

journal_title：FEBS letters

authors： Puri NK,Cardamone M

更新日期：1992-07-06 00:00:00