Abstract:
:Phenylalanine-specific tRNA from yeast was hydrolysed with cobra venom ribonuclease in the double-stranded regions and the fragments isolated. The 'dissected' molecules with nicks in positions 28 and 41 were reconstructed from supplementary fragments and treated with T-4 RNA ligase. A phosphodiester bond between two fragments was formed when the fragment combination (1-28) + (29-76) was used. A strong discrimination in the ligation yield between different nick positions in the same helix is shown.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Boutorin AS,Vassilenko SK,Baklanov MM,Nechaev YSdoi
10.1016/0014-5793(84)80021-6subject
Has Abstractpub_date
1984-01-02 00:00:00pages
93-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(84)80021-6journal_volume
165pub_type
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