Transcriptional repression by methylation: cooperativity between a CpG cluster in the promoter and remote CpG-rich regions.


:Cytosine methylation of binding sites for transcription factors is a straightforward mechanism to prevent transcription, while data on an indirect mechanism, by methylation outside of the factor binding sites, are still scarce. We have studied the latter effect using a model promoter construct. For this, a 69 bp G + C rich DNA segment with a cluster of 14 CpG sites was inserted between upstream lexA sites and the TATA box. Transcription was measured in transient transfection assays with lexA-VP16 as an activating factor. When the entire plasmid was methylated at all CpGs before transfection, transcription was blocked (to 3% residual activity), whereas transcription was only mildly inhibited (to 60%) by methylation of a control plasmid that lacked the 69 bp CpG cluster. However, the effect could not simply be attributed to methylation of the CpG cluster: neither a methylated CpG cluster in an otherwise methylation-free reporter gene plasmid, nor the methylated plasmid with an unmethylated CpG cluster, inhibited transcription considerably (69% and 44% remaining activity, respectively). The data presented here suggest that a minimal length of methylated DNA in the promoter is required for repression, and imply that concomitant methylation of CpGs in the promoter region and in remote sequences can cooperatively block transcription, without the need to methylate any binding sites for transcription factors. We also note that the cooperation for a negative effect described here bears an analogy to transcriptional activation, where a promoter often cooperates with a remote enhancer.




FEBS letters


Hug M,Silke J,Georgiev O,Rusconi S,Schaffner W,Matsuo K




Has Abstract


1996-02-05 00:00:00














  • Overexpression of the jasmonate-inducible 23 kDa protein (JIP 23) from barley in transgenic tobacco leads to the repression of leaf proteins.

    abstract::We investigated transgenic tobacco lines which express different amounts of the barley JIP 23. In these plants the amount of several proteins decreased proportionally to increasing amounts of JIP 23 whereas the transcript levels were constant as determined for the small and the large subunit of RuBPCase. However, the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Görschen E,Dunaeva M,Reeh I,Wasternack C

    更新日期:1997-12-08 00:00:00

  • Meprins process matrix metalloproteinase-9 (MMP-9)/gelatinase B and enhance the activation kinetics by MMP-3.

    abstract::Meprin α and β, members of the astacin family of zinc metalloproteinases, are unique plasma membrane and secreted proteases known to cleave a wide range of biological substrates involved in inflammation, cancer and fibrosis. In this study, we identified proMMP-9 as a novel substrate and show that aminoterminal meprin-...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Geurts N,Becker-Pauly C,Martens E,Proost P,Van den Steen PE,Stöcker W,Opdenakker G

    更新日期:2012-12-14 00:00:00

  • Generation of VV-hemorphin-7 from globin by peritoneal macrophages.

    abstract::Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dagouassat N,Garreau I,Sannier F,Zhao Q,Piot JM

    更新日期:1996-03-11 00:00:00

  • Hyperoxia-mediated oxidative stress increases expression of UCP3 mRNA and protein in skeletal muscle.

    abstract::The uncoupling protein-3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated the effect of an oxidative stress induced by hyp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Flandin P,Donati Y,Barazzone-Argiroffo C,Muzzin P

    更新日期:2005-06-20 00:00:00

  • The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa.

    abstract::The nucleotide sequence of the amiE gene, encoding the aliphatic amidase of Pseudomonas aeruginosa, has been determined. The sequence of 1038 nucleotides shows a strong bias in favour of codons with G or C in the third position, and only 44 different codons are utilised. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brammar WJ,Charles IG,Matfield M,Liu CP,Drew RE,Clarke PH

    更新日期:1987-05-11 00:00:00

  • Silencing of filamin A gene expression inhibits Ca2+ -sensing receptor signaling.

    abstract::Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Huang C,Wu Z,Hujer KM,Miller RT

    更新日期:2006-03-20 00:00:00

  • Temperature and cryoprotectant influence secondary quinone binding position in bacterial reaction centers.

    abstract::We have determined the first de novo position of the secondary quinone QB in the Rhodobacter sphaeroides reaction center (RC) using phases derived by the single wavelength anomalous dispersion method from crystals with selenomethionine substitution. We found that in frozen RC crystals, QB occupies primarily the proxim...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pokkuluri PR,Laible PD,Crawford AE,Mayfield JF,Yousef MA,Ginell SL,Hanson DK,Schiffer M

    更新日期:2004-07-16 00:00:00

  • Unique sequence of a high molecular weight myosin light chain kinase is involved in interaction with actin cytoskeleton.

    abstract::Myosin light chain kinase (MLCK) is the key regulator of cell motility and smooth muscle contraction in higher vertebrates. We searched for the features of the high molecular weight MLCK (MLCK-210) associated with its unique N-terminal sequence not found in a more ubiquitous lower molecular weight MLCK (MLCK-108). MLC...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kudryashov DS,Chibalina MV,Birukov KG,Lukas TJ,Sellers JR,Van Eldik LJ,Watterson DM,Shirinsky VP

    更新日期:1999-12-10 00:00:00

  • Modelling, synthesis and biological activity of a BLV proteinase, made of (only) 116 amino acids.

    abstract::Bovine leukaemia virus (BLV) is the aetiological agent of Leukosis enzootica bovis [Viral Oncology (1980), G. Klein (Ed.) Raven Press, New York, pp. 231-238], a widely spread disease in cattle. BLV is reported as the animal model of human T-cell leukaemia virus (HLTV) which is the causative agent of adult T-cell leuka...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Précigoux G,Geoffre S,Léonard R,Llido S,Dautant A,d'Estaintot BL,Picard P,Ménard A,Guillemain B,Hospital M

    更新日期:1993-07-12 00:00:00

  • The cytochrome bc1 complex as an antipathogenic target.

    abstract::The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Fisher N,Meunier B,Biagini GA

    更新日期:2020-09-01 00:00:00

  • Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.

    abstract::We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

    更新日期:2016-09-01 00:00:00

  • The monofunctional glycosyltransferase of Escherichia coli is a member of a new class of peptidoglycan-synthesising enzymes.

    abstract::Using conserved fingerprints in the glycosyltransferase (GTase) domain of high-molecular-weight penicillin-binding proteins (PBP), a gene (mgt) encoding a putative monofunctional glycosyltransferase has been identified in Haemophilus influenzae and in other bacteria] species. Here we report the cloning of the homologo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Di Berardino M,Dijkstra A,Stüber D,Keck W,Gubler M

    更新日期:1996-08-26 00:00:00

  • Human plasma kininogens are identical with alpha-cysteine proteinase inhibitors. Evidence from immunological, enzymological and sequence data.

    abstract::Human high- and low-Mr kininogens were shown to be potent inhibitors of cysteine proteinases such as cathepsin L and papain (Ki = 17-48 pM). A strong immunological cross-reaction between the kininogens and low-Mr alpha-cysteine proteinase inhibitor from human plasma was found. Comparison of partial amino acid sequence...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Müller-Esterl W,Fritz H,Machleidt W,Ritonja A,Brzin J,Kotnik M,Turk V,Kellermann J,Lottspeich F

    更新日期:1985-03-25 00:00:00

  • Preparation and structure of the charge-transfer intermediate of the transmembrane redox catalyst DsbB.

    abstract::Disulfide bond formation is a critical step in the folding of many secretory proteins. In bacteria, disulfide bonds are introduced by the periplasmic dithiol oxidase DsbA, which transfers its catalytic disulfide bond to folding polypeptides. Reduced DsbA is reoxidized by ubiquinone Q8, catalyzed by inner membrane quin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Malojcić G,Owen RL,Grimshaw JP,Glockshuber R

    更新日期:2008-10-15 00:00:00

  • A recombinant polypeptide model of the second predicted nucleotide binding fold of the cystic fibrosis transmembrane conductance regulator is a GTP-binding protein.

    abstract::Association reactions of a recombinant CFTR-NBF-2 polypeptide fused to glutathione S-transferase with guanine nucleotides were monitored quantitatively by recording the fluorescence enhancement of excited trinitrophenol (TNP)-labelled GTP after binding to NBF-2. Binding of TNP-GTP to the recombinant NBF-2 polypeptide ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Randak C,Neth P,Auerswald EA,Assfalg-Machleidt I,Roscher AA,Hadorn HB,Machleidt W

    更新日期:1996-11-25 00:00:00

  • Effects of a novel peptide derived from human thrombomodulin on endotoxin-induced uveitis in vitro and in vivo.

    abstract::Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jin H,Yang X,Liu K,Gu Q,Xu X

    更新日期:2011-11-04 00:00:00

  • A putative internal promoter in the 16 S/23 S intergenic spacer of the rRNA operon of archaebacteria and eubacteria.

    abstract::The existence of the internal promoter Pi in the 16 S/23 S intergenic spacers of the rRNA operons of an eubacterium Escherichia coli and archaebacterium Halobacterium halobium is proposed. The possible functional significance of these promoters is discussed. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mankin AS,Skripkin EA,Kagramanova VK

    更新日期:1987-07-27 00:00:00

  • The active sites of cellulases are involved in chiral recognition: a comparison of cellobiohydrolase 1 and endoglucanase 1.

    abstract::The cellulases cellobiohydrolase 1 (CBH 1) and endoglucanase 1 (EG 1) from the fungus Trichoderma reesei are closely related with 40% sequence identity and very similar in structure. In CBH 1 the active site is enclosed by long loops and some antiparallel beta-strands forming a 40 A long tunnel, whereas in EG 1 part o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Henriksson H,Ståhlberg J,Isaksson R,Pettersson G

    更新日期:1996-07-29 00:00:00

  • Expression of a Cu,Zn superoxide dismutase typical of familial amyotrophic lateral sclerosis induces mitochondrial alteration and increase of cytosolic Ca2+ concentration in transfected neuroblastoma SH-SY5Y cells.

    abstract::We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

    更新日期:1997-09-08 00:00:00

  • Identification of LRP1B-interacting proteins and inhibition of protein kinase Calpha-phosphorylation of LRP1B by association with PICK1.

    abstract::Recent studies show LDL receptor-related protein 1B, LRP1B as a transducer of extracellular signals. Here, we identify six interacting partners of the LRP1B cytoplasmic region by yeast two-hybrid screen and confirmed their in vivo binding by immunoprecipitation. One of the partners, PICK1 recognizes the C-terminus of ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shiroshima T,Oka C,Kawaichi M

    更新日期:2009-01-05 00:00:00

  • Amino acid sequence of equine platelet tropomyosin. Correlation with interaction properties.

    abstract::Equine platelet beta tropomyosin (247 residues), like rabbit skeletal muscle alpha tropomyosin (284 residues) has a repeating pattern of amino acid residues characteristic of a coiled-coil structure. When compared with the muscle protein, it is extended by 5 residues at the NH2-terminus and possesses two 21 residue de...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lewis WG,Cote GP,Mak AS,Smillie LB

    更新日期:1983-06-13 00:00:00

  • Identification and characterization of secreted factors that are upregulated during somatic cell reprogramming.

    abstract::The process of cell reprogramming has been characterized considerably since the successful generation of induced pluripotent stem cells. However, the importance of cell-cell communications for cellular reprogramming remains largely unknown. Secreted factors, which are expressed and secreted during reprogramming, may i...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Bansho Y,Lee J,Nishida E,Nakajima-Koyama M

    更新日期:2017-06-01 00:00:00

  • BRCA1 interacts with FHL2 and enhances FHL2 transactivation function.

    abstract::Germ-line mutations in BRCA1 are associated with an increased lifetime risk of developing breast and/or ovarian tumors. The BRCA1 gene product is a 220-kDa protein that contains a tandem of two BRCA1 C-terminal (BRCT) domains required for transcription. In an attempt to understand how BRCA1 exerts its function through...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yan J,Zhu J,Zhong H,Lu Q,Huang C,Ye Q

    更新日期:2003-10-09 00:00:00

  • The role of the C-terminal domain in the inhibitory functions of tissue factor pathway inhibitor.

    abstract::Tissue factor pathway inhibitor (TFPI) inhibits the activity of coagulation factors VIIa and Xa through Kunitz domains, thereby inhibiting the activity of tissue factor. However, it has been shown that the C-terminal of this inhibitor is essential for the maximal anticoagulant activity of TFPI. We have investigated th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ettelaie C,Adam JM,James NJ,Oke AO,Harrison JA,Bunce TD,Bruckdorfer KR

    更新日期:1999-12-17 00:00:00

  • Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3.

    abstract::The glycogen-binding (G) subunit of protein phosphatase-1G is phosphorylated stoichiometrically by glycogen synthase kinase-3 (GSK3), and with a greater catalytic efficiency than glycogen synthase, but only after prior phosphorylation by cyclic AMP-dependent protein kinase (A-kinase) at site 1. The residues phosphoryl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dent P,Campbell DG,Hubbard MJ,Cohen P

    更新日期:1989-05-08 00:00:00

  • Le couplage phosphore-phosphore dans l'adenosine di- et triphosphate.

    abstract::The pH dependence of spin-spin coupling constants J (P-O-P) of ADP and ATP has been determined from the phosphorus NMR spectra at 0 degrees. The variation curves have been interpreted as titration curves. These give different pK values. Comparison with the tripolyphosphate shows the existence of triphosphate chain str...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ellenberger M,Brehamet L,Villemin M,Toma F

    更新日期:1970-06-01 00:00:00

  • High hydrostatic pressure activates gene expression through Msn2/4 stress transcription factors which are involved in the acquired tolerance by mild pressure precondition in Saccharomyces cerevisiae.

    abstract::Msn2 and Msn4 transcription factors activate expression of stress-responsive element (STRE) controlled genes in response to various stresses triggering the environmental stress response in Saccharomyces cerevisiae. Although high hydrostatic pressure is known to induce gene expression modification in yeast, the transcr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Domitrovic T,Fernandes CM,Boy-Marcotte E,Kurtenbach E

    更新日期:2006-11-13 00:00:00

  • Sizing of single-stranded regions in double-stranded DNA by preparative benzoylated DEAE-cellulose chromatography.

    abstract::The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybri...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Norris MD,Stewart BW

    更新日期:1988-02-15 00:00:00

  • Mechanism of transport of L-alanine by luminal-membrane vesicles from pars recta of rabbit proximal tubule.

    abstract::The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na+ requiring systems confined to this region of the nephron exist for the transpor...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vorum H,Jessen H,Jørgensen KE,Sheikh MI

    更新日期:1988-01-18 00:00:00

  • A novel combination of prosthetic groups in a fungal laccase; PQQ and two copper atoms.

    abstract::Extracellular laccase (benzenediol: oxygen oxidoreductase EC from the lignin-degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Karhunen E,Niku-Paavola ML,Viikari L,Haltia T,van der Meer RA,Duine JA

    更新日期:1990-07-02 00:00:00