An inhibitory effect of actin on casein kinase II activity in vitro.

abstract：:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...

journal_title：FEBS letters

authors： Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser T

更新日期：2017-09-01 00:00:00

abstract：:Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method...

journal_title：FEBS letters

authors： Kalipatnapu S,Chattopadhyay A

更新日期：2004-10-22 00:00:00

abstract：:The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...

journal_title：FEBS letters

authors： Sirvent A,Claudel T,Martin G,Brozek J,Kosykh V,Darteil R,Hum DW,Fruchart JC,Staels B

更新日期：2004-05-21 00:00:00

abstract：:Liposomes can be loaded with weak acids and bases, which exist in solutions in equilibrium with membrane permeable uncharged form, using various gradients across their membranes. Because in some cases the estimated drug concentration in the loaded liposomes exceeds their aqueous solubility we investigated the physical...

journal_title：FEBS letters

authors： Lasic DD,Frederik PM,Stuart MC,Barenholz Y,McIntosh TJ

更新日期：1992-11-09 00:00:00

abstract：:alpha-Dystroglycan was quantitatively enriched from mammalian brain based on its uniform reactivity with Vicia villosa agglutinin and resolved into sub-populations possessing or lacking the sulfated glucuronic acid epitope recognized by monoclonal antibody HNK-1. We generated a new monoclonal antibody specific for a g...

journal_title：FEBS letters

authors： McDearmon EL,Combs AC,Sekiguchi K,Fujiwara H,Ervasti JM

更新日期：2006-06-12 00:00:00

abstract：:The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...

journal_title：FEBS letters

authors： Song KS,Peterson YK,Freidman A,Blumer JB,Sato M,Lanier SM

更新日期：2006-04-03 00:00:00

abstract：:In response to osmotic cell swelling, Intestine 407 cells react with a rapid and transient activation of phospholipase D (PLD). To investigate the role of PLD during the regulatory volume decrease, cells were treated with 1-butanol resulting in a depletion of PLD substrates. Activation of volume-regulated anion channe...

journal_title：FEBS letters

authors： Tomassen SF,van der Wijk T,de Jonge HR,Tilly BC

更新日期：2004-05-21 00:00:00

abstract：:Unstimulated human fibrosarcoma cells (HT1080) constitutively secrete matrix metalloproteinase 2 (MMP 2) as a proenzyme requiring proteolytic cleavage by membrane type-1 MMP (MT1 MMP) for activation. Physiological and pharmacological stimuli induce clustering of MT1 MMP/tissue inhibitor of MMP 2 "receptors", promoting...

journal_title：FEBS letters

authors： Atkinson SJ,English JL,Holway N,Murphy G

更新日期：2004-05-21 00:00:00

abstract：:Post-transcriptional regulation of gene expression by RNA-binding proteins and by small non-coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch-2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RN...

journal_title：FEBS letters

authors： Boudoukha S,Rivera Vargas T,Dang I,Kropp J,Cuvellier S,Gautreau A,Polesskaya A

更新日期：2014-05-02 00:00:00

abstract：:Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

journal_title：FEBS letters

authors： Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

更新日期：2005-02-28 00:00:00

abstract：:The degradation of type IX collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (MMPs) purified from the culture medium of rheumatoid synovial cells. Neither MMP-1 (collagenase) nor MMP-2 (so-called 'gelatinase') could digest type IX collagen, but M...

journal_title：FEBS letters

authors： Okada Y,Konomi H,Yada T,Kimata K,Nagase H

更新日期：1989-02-27 00:00:00

abstract：:The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin,...

journal_title：FEBS letters

authors： Baldwin GS

更新日期：1995-02-06 00:00:00

abstract：:Cellulysin, a crude cellulase from the plant parasitic fungus Trichoderma viride, induces the biosynthesis of volatiles in higher plants (Nicotiana plumbaginifolia, Phaseolus lunatus, and Zea mays) when applied to cut petioles by the transpiration stream. The pattern of the emitted volatiles largely resembles that fro...

journal_title：FEBS letters

authors： Piel J,Atzorn R,Gäbler R,Kühnemann F,Boland W

更新日期：1997-10-20 00:00:00

abstract：:Siamycin I disrupts growth and quorum sensing in Enterococcus faecalis. Using purified intact protein, we demonstrate here that quorum membrane sensor kinase FsrC is a direct target of siamycin I, reducing pheromone-stimulated autophosphorylation activity by up to 91%. Inhibition was non-competitive with ATP as substr...

journal_title：FEBS letters

authors： Ma P,Nishiguchi K,Yuille HM,Davis LM,Nakayama J,Phillips-Jones MK

更新日期：2011-09-02 00:00:00

abstract：:The rapid conversion of the carotenoid violaxanthin to zeaxanthin via antheraxanthin (xanthophyll cycle) in potato leaves exposed at 23 degrees C to a strong white light of 2000 microE.m-2.s-1 was associated with a slight inhibition of photosynthetic electron transport (as estimated from chlorophyll fluorescence measu...

journal_title：FEBS letters

authors： Sarry JE,Montillet JL,Sauvaire Y,Havaux M

更新日期：1994-10-17 00:00:00

abstract：:Recently, we have reported the presence of kinetin (N6-furfuryladenine) in commercially available DNA, in freshly extracted cellular DNA and in plant cell extracts. We have also found that kinetin has electrochemical properties which can be used for monitoring the level of this modified base in DNA. Here, for the firs...

journal_title：FEBS letters

authors： Barciszewski J,Siboska GE,Pedersen BO,Clark BF,Rattan SI

更新日期：1997-09-08 00:00:00

abstract：:Changes in membrane expression of sodium-dependent glucose transporter (SGLT1) and glucose transporter isoform (GLUT2) protein have been implicated in the increased intestinal glucose transport in streptozotocin-diabetes. The possible involvement of GLUT1 in the transport response, however, has not previously been stu...

journal_title：FEBS letters

authors： Boyer S,Sharp PA,Debnam ES,Baldwin SA,Srai SK

更新日期：1996-11-04 00:00:00

abstract：:In human brain the Abeta peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Abeta secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which over...

journal_title：FEBS letters

authors： Recuero M,Serrano E,Bullido MJ,Valdivieso F

更新日期：2004-07-16 00:00:00

abstract：:The E-E* model for calcium pumping by the CaATPase of sarcoplasmic reticulum includes two distinct conformational states of the enzyme, E and E*. Exterior Ca2+ binds only to E and interior Ca2+ binds only to E*. Therefore, it is expected that there will be competition between the binding of calcium to the unphosphoryl...

journal_title：FEBS letters

authors： Myung J,Jencks WP

更新日期：1991-01-14 00:00:00

abstract：:MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in thi...

journal_title：FEBS letters

authors： Miao LJ,Huang SF,Sun ZT,Gao ZY,Zhang RX,Liu Y,Wang J

更新日期：2013-05-02 00:00:00

abstract：:Curcumin, the yellow pigment from Curcuma longa, has been shown to possess tumoricidal activity. We have earlier reported the induction of apoptosis in AK-5, rat histiocytic cells by curcumin leading to the inhibition of tumor growth in vivo. In this study we have observed differential activation status in host macrop...

journal_title：FEBS letters

authors： Bhaumik S,Jyothi MD,Khar A

更新日期：2000-10-13 00:00:00

abstract：:The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...

journal_title：FEBS letters

authors： Yamaguchi N,Yamaguchi N

更新日期：2015-05-22 00:00:00

abstract：:Gibberellins are ent-kaurene-derived diterpenoid phytohormones produced by plants, fungi, and bacteria. The distinct gibberellin biosynthetic pathways in plants and fungi are known, but not that in bacteria. Plants typically use two diterpene synthases to form ent-kaurene, while fungi use only a single bifunctional di...

journal_title：FEBS letters

authors： Morrone D,Chambers J,Lowry L,Kim G,Anterola A,Bender K,Peters RJ

更新日期：2009-01-22 00:00:00

abstract：:The DNA binding characteristics of a mono-, di- and trimeric derivative of 9-aminoacridine were studied. The length of the linking carboxamidoalkyl chains was selected to allow bis- or tris-intercalation according to the excluded-site model. Measurements of DNA unwinding angle using closed circular DNA showed that the...

journal_title：FEBS letters

authors： Gaugain B,Markovits J,Le Pecq JB,Roques BP

更新日期：1984-04-24 00:00:00

abstract：:Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...

journal_title：FEBS letters

authors： Tanfin Z,Goureau O,Milligan G,Harbon S

更新日期：1991-01-14 00:00:00

abstract：:Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca(2+) release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca(2+) dependence in [(3)H]ryanodine b...

journal_title：FEBS letters

authors： Nakai J,Gao L,Xu L,Xin C,Pasek DA,Meissner G

更新日期：1999-10-08 00:00:00

abstract：:A pulsed field gradient three-dimensional isotope-filtered 13C HMQC-NOESY experiment has been developed to characterize intermolecular contacts in a 37 kDa macromolecular ternary complex consisting of uniformly 13C labeled trp-repressor, its natural abundance co-repressor, L-tryptophan, and natural abundance operator ...

journal_title：FEBS letters

authors： Lee W,Revington MJ,Arrowsmith C,Kay LE

更新日期：1994-08-15 00:00:00

abstract：:Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

journal_title：FEBS letters

authors： Pepe IM,Panfoli I,Cugnoli C

更新日期：1986-07-14 00:00:00

abstract：:The ability of three fatty acids and their respective ethyl esters, to promote generation of reactive oxygen species (ROS), was compared in a preparation of rat brain synaptosomes. Arachidonic but not palmitic or linoleic acids promoted ROS generation. Ethyl esterification of each fatty acid significantly enhanced ROS...

journal_title：FEBS letters

authors： Bondy SC,Marwah S

更新日期：1995-11-13 00:00:00

abstract：:To address biophysical principles and lipid interactions that underlie the properties of membrane proteins, modifications that vary the neighbors of tryptophan residues in the highly dynamic transmembrane helix of GW4,20 ALP23 (acetyl-GGAW4 A(LA)6 LAW20 AGA-amide) were examined using deuterium NMR spectroscopy. It was...

journal_title：FEBS letters

authors： McKay MJ,Greathouse DV,Koeppe RE 2nd

更新日期：2020-12-01 00:00:00