Abstract:
:Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length enhances the telomerase inhibitory potential, but decreases specificity, indicated by HIV reverse transcriptase inhibition. Chimeras with 5' (s4dU)(n)s were more potent inhibitors than the antisense alone or the 3' modified ones. Cy5-labeled (s4dU)4AS and (s4dU)8AS proved the internalization of the oligonucleotides, raising the possibility to be tested as cellular anti-telomerase agents.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi Jdoi
10.1016/j.febslet.2005.01.041keywords:
subject
Has Abstractpub_date
2005-02-28 00:00:00pages
1411-6issue
6eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00140-7journal_volume
579pub_type
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