Abstract:
:The whole-cell patch-clamp technique was used to measure cell membrane capacitance (Cm) to monitor exocytosis in single-cultured bovine prolactin-secreting cells (lactotrophs) of the anterior pituitary. The cells were dialyzed with solutions containing different concentrations of ionised Ca and non-hydrolyzable GTP analogues (GTP-gamma-S and GMP-PNP) to activate G-proteins. We have identified two distinct effects of G-protein activation on Ca-induced exocytosis: (i) the maximum Cm increase due to intracellular Ca-dependent exocytosis was diminished, suggesting an inhibitory role of G-proteins close to the site of granule fusion, while (ii) the rate of Cm increase (delta Cm/delta t) was facilitated, revealing conversely a stimulatory role of G-proteins in the translocation of secretory granules to the fusion sites.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sikdar SK,Zorec R,Brown D,Mason WTdoi
10.1016/0014-5793(89)80936-6subject
Has Abstractpub_date
1989-08-14 00:00:00pages
88-92issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80936-6journal_volume
253pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80646-9
更新日期:1970-10-16 00:00:00
abstract::Adaptation to hypoxia is essential for tumor progression. Transcriptional activation of hypoxia-regulated genes is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1alpha and ARNT (Ah receptor nuclear translocator; HIF-1beta). Using representational difference analysis, we identified three novel hy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03475-0
更新日期:2002-10-23 00:00:00
abstract::Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01724-x
更新日期:2000-01-07 00:00:00
abstract::Activation of p53 plays a central role in the cell's response to various stress signals. We investigated whether p53 is activated upon disruption of actin microfilaments, caused by cytochalasin D (CD). We show that treatment with CD leads to accumulation of p53 in the cells and activation of p53-dependent transcriptio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00692-9
更新日期:1998-07-03 00:00:00
abstract::Regulation of supra-macromolecular composition and catalytic activity of a heterodimeric enzyme, gamma-glutamyltransferase, in the system of Aerosol OT (sodium bis(2-ethylhexyl) sulfosuccinate) reversed micelles in octane were studied. Variation of the surfactant hydration degree (parameter, determining dimensions of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81388-o
更新日期:1991-12-16 00:00:00
abstract::The structural characteristics of the three nuclear phosphoproteins of the high mobility group A family are outlined and related to their participation in chromatin structure alteration in many biological processes such as gene expression, neoplastic transformation, differentiation, and apoptosis. The elevated express...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2004.08.013
更新日期:2004-09-10 00:00:00
abstract::Several types of post-translational modifications contribute to the high level of tubulin heterogeneity in the brain. An important modification is glutamylation of the major brain-specific isotypes, such as class Ia/b of alpha-tubulin and classes II and III of beta-tubulin. Here we describe experiments to determine if...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01018-8
更新日期:1994-10-10 00:00:00
abstract::Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81007-b
更新日期:1990-07-30 00:00:00
abstract::The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.008
更新日期:2007-02-06 00:00:00
abstract::Effects of amino acid substitutions in the first extracellular loop region of the delta- and mu-opioid receptors were examined. Substitution of lysine-108 of the delta-receptor (delta K108) with asparagine improved affinity to [D-Ala2,MePhe4,Gly-ol5]enk ephalin (DAGO), a mu-selective peptide agonist, to be comparable ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01034-c
更新日期:1995-10-09 00:00:00
abstract::A Ca2+ indicator arsenazo III was used to demonstrate calcium uptake activity of symbiosomes and the peribacteroid membrane (PBM) vesicles isolated from broad bean root nodules and placed in the medium containing ATP and Mg2+ ions. This process was shown to be rapidly stopped by vanadate, completely reversed in the pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00262-8
更新日期:1999-03-19 00:00:00
abstract::Recent studies have shown that p21-activated kinase 1 (Pak1) phosphorylates estrogen receptor-alpha (ER alpha) at Ser 305 and also promotes its transactivation function. Here, we sought to investigate whether substitution of serine 305 in ER with glutamic acid (ER alpha-S305E), which mimics the phosphorylation state, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.071
更新日期:2004-06-04 00:00:00
abstract::Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00821-p
更新日期:1995-09-04 00:00:00
abstract::Co-infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) is common and is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma (HCC). Here, we demonstrated that HBV X protein (HBx) and HCV core protein additively repress the universal cyclin-de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02694-7
更新日期:2002-05-08 00:00:00
abstract::The human pathogen Vibrio vulnificus undergoes phase variation among colonial morphotypes, including a virulent opaque form which produces capsular polysaccharide (CPS) and a translucent phenotype that produces little or no CPS and is attenuated. Here, we found that a V. vulnificus mutant defective for RfaH antitermin...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12490
更新日期:2016-12-01 00:00:00
abstract::Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.09.105
更新日期:2006-02-06 00:00:00
abstract::The stomach form of alcohol dehydrogenase has been structurally evaluated by peptide analysis covering six separate regions of the rat enzyme. Overall, this new structure differs widely (32-40% residue differences) from the structures of three classes of alcohol dehydrogenase characterized before from the same species...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80822-z
更新日期:1990-12-17 00:00:00
abstract::Screening of hemorphins in extracts of rat lung, brain, heart and spleen was carried out. The threshold for detection of hemorphins was 0.01 nmol for spleen and 0.05 nmol for other tissues. Both the content and the composition of hemorphins differed significantly in the tissues analyzed. Heart and lung extracts were r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00549-3
更新日期:1998-05-29 00:00:00
abstract::Kinetic analyses of cpTat-mediated protein transport across the thylakoid membrane were conducted, revealing three important characteristics of this translocation pathway. First, transport via the cpTAT system displays a non-Michaelis-Menten, sigmoidal rate-substrate relationship with an apparent Hill coefficient of 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00231-x
更新日期:2003-04-10 00:00:00
abstract::All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2008.04.029
更新日期:2008-06-18 00:00:00
abstract::A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed prote...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80576-3
更新日期:1988-02-08 00:00:00
abstract::The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.03.083
更新日期:2006-05-22 00:00:00
abstract::The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1x10(6) or 1x10(5) B16BL6 cells were injected into m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01144-3
更新日期:2000-02-11 00:00:00
abstract::We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81547-d
更新日期:1993-05-10 00:00:00
abstract::The effect of neuropeptide Y (NPY) on cytosolic free Ca2+ concentration ([Ca2+]i) was studied in cultured smooth muscle cells from porcine aorta (PASMC) and compared with the effect of bradykinin (BK) and angiotensin II (ATII) on [Ca2+]i. All peptides induced dose-dependent and transient rises in [Ca2+]i which were no...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81499-1
更新日期:1989-12-18 00:00:00
abstract::Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid and glucose homeostasis. Here we show that FXR is expressed and functional, as assessed by target gene expression analysis, in human islets and beta-cell lines. FXR is predominantly cytosolic-localized in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.068
更新日期:2010-07-02 00:00:00
abstract::Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.001
更新日期:2008-02-06 00:00:00
abstract::The gap junctional intercellular communication mediated by Cx43 plays indispensable roles in both germ line development and postnatal folliculogenesis. In this study, we focused on the effect of follicle-stimulating hormone (FSH) on the Cx43 protein in rat primary granulosa cells and found that FSH stimulation elevate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03441-5
更新日期:2002-11-06 00:00:00
abstract::We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.080
更新日期:2007-05-29 00:00:00
abstract::A novel rapid polymerase chain reaction (PCR)-based technique for N-terminal attachment of green fluorescent protein (GFP) to a yeast protein is described. Genomic integration of a PCR-generated loxPkanMX4loxP-yEGFP fusion cassette immediately upstream of the open reading frame (ORF) allows for selection of G418 resis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02179-7
更新日期:2000-11-17 00:00:00