Nuclear phosphoproteins HMGA and their relationship with chromatin structure and cancer.

abstract：:A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensiona...

journal_title：FEBS letters

authors： Nimtz M,Wray V,Rüdiger A,Conradt HS

更新日期：1995-05-29 00:00:00

abstract：:An improved method for expressing and purifying bovine pancreatic ribonuclease from a synthetic gene using the lambda promoter controlled by a temperature-sensitive repressor is described. The procedure involves isolation in the presence of a refolding buffer containing oxidized and reduced glutathione, under conditio...

journal_title：FEBS letters

authors： McGeehan GM,Benner SA

更新日期：1989-04-10 00:00:00

abstract：:The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...

journal_title：FEBS letters

authors： Byrne AM,Elliott C,Hoffmann R,Baillie GS

更新日期：2015-03-12 00:00:00

abstract：:The flash-induced voltage response of halorhodopsin at high NaCl concentration comprises two main kinetic components. The first component with tau approximately 1 micros does not exceed 4% of the overall response amplitude and is probably associated with the formation of the L (hR520) intermediate. The second main com...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kalaidzidis YL,Kaulen AD

更新日期：1998-05-01 00:00:00

abstract：:Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA. Glycosylation of the enzymes does not significantly affect their kinetic properties, or their stabilities towards heat and pH. However, the glycosylated enzymes ar...

journal_title：FEBS letters

authors： Langsford ML,Gilkes NR,Singh B,Moser B,Miller RC Jr,Warren RA,Kilburn DG

更新日期：1987-12-10 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

journal_title：FEBS letters

authors： Hecker M,Walsh DT,Vane JR

更新日期：1991-12-09 00:00:00

abstract：:A-to-I RNA editing can lead to recoding of pre-mRNAs with profound functional consequences for the ensuing proteins. Here we show that complement component 1, q subcomponent-like 1 (C1QL1) undergoes RNA editing in vivo causing non-synonymous amino acid substitutions in human, mouse as well as zebrafish. The major edit...

journal_title：FEBS letters

authors： Sie CP,Maas S

更新日期：2009-04-02 00:00:00

abstract：:The range of delta-selectivity of linear and cyclic analogues of enkephalin in rat brain was found to be: [D-Pen2, L-Pen5] enkephalin (DPLPE) greater than [D-Pen2, D-Pen5] enkephalin (DPDPE) greater than [D-Thr2, Leu5] enkephalyl-Thr6 (DTLET) greater than [D-Ser2, Leu5] enkephalyl-Thr6 (DSLET). Saturation experiments ...

journal_title：FEBS letters

authors： Delay-Goyet P,Zajac JM,Rigaudy P,Foucaud B,Roques BP

更新日期：1985-04-22 00:00:00

abstract：:Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activat...

journal_title：FEBS letters

authors： Jezek P,Modrianský M,Garlid KD

更新日期：1997-05-19 00:00:00

abstract：:α-Synuclein (αSyn) is a key player in the pathogenesis of Parkinson's disease and other synucleinopathies. Here, we report the existence of a novel soluble α-helical conformer of αSyn, obtained through transient interaction with lipid interfaces, and propose dynamic oligomerization as the mechanism underlying its stab...

journal_title：FEBS letters

authors： Rovere M,Sanderson JB,Fonseca-Ornelas L,Patel DS,Bartels T

更新日期：2018-05-01 00:00:00

abstract：:We report for the first time the identification of 25 microRNAs from tissues originating from chicken embryo and adult chicken. Most of the cloned microRNAs are expressed in both adult chickens and chicken embryos. Fourteen were identified without any prior prediction. One microRNA, miR-757, is thought to be chicken-s...

journal_title：FEBS letters

authors： Xu H,Wang X,Du Z,Li N

更新日期：2006-06-26 00:00:00

abstract：:We have examined the formation of DNA triple helices between the oligonucleotides T8XT8 (X = A,C,G,T) and DNA fragments containing the target sequences A8XA8.T8YT8 (X = T,C,G; Y = A,G,C), by DNase I footprinting. We find that A8GA8.T8CT8 yields a footprint with T8CT8 and shows a weaker interaction with T17 and T8GT8. ...

journal_title：FEBS letters

authors： Chandler SP,Fox KR

更新日期：1993-10-11 00:00:00

abstract：:We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...

journal_title：FEBS letters

authors： Kusakabe T,Suzuki N

更新日期：2000-10-20 00:00:00

abstract：:There is considerable epidemiological evidence that tea drinking lowers the risk of heart disease. However, the mechanism by which tea can be protective is unknown. Hamsters were fed a normal or high cholesterol diet for 2 weeks and drank green or black tea ad libitum. The plasma lipid profile was significantly improv...

journal_title：FEBS letters

authors： Vinson JA,Dabbagh YA

更新日期：1998-08-14 00:00:00

abstract：:The cardiovascular system consists of many cell types with distinct embryonic origins. Cells from an Islet1 (Isl1)-expressing progenitor population make a substantial contribution to the developing heart. We reasoned that cells derived from Isl1-expressing progenitors might contribute more widely to the cardiovascular...

journal_title：FEBS letters

authors： Keenan ID,Rhee HJ,Chaudhry B,Henderson DJ

更新日期：2012-06-21 00:00:00

abstract：:The bacterial twin-arginine translocation (Tat) pathway is distinct from the Sec system by its remarkable capacity to export folded enzymes. To address the question whether the two systems are capable of translocating homologous enzymes catalyzing the same reaction, we cloned the tap gene encoding Thermus thermophilus...

journal_title：FEBS letters

authors： Angelini S,Moreno R,Gouffi K,Santini C,Yamagishi A,Berenguer J,Wu L

更新日期：2001-10-05 00:00:00

abstract：:The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

journal_title：FEBS letters

authors： Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

更新日期：2004-07-16 00:00:00

abstract：:Uptake of taurine in human placental brush-border membrane vesicles was greatly stimulated in the presence of an inwardly-directed Na+ + Cl- -gradient and uphill transport of taurine could be demonstrated under these conditions. Na+ as well as Cl- were obligatory for this uptake and both ion gradients could energize t...

journal_title：FEBS letters

authors： Miyamoto Y,Balkovetz DF,Leibach FH,Mahesh VB,Ganapathy V

更新日期：1988-04-11 00:00:00

abstract：:It is shown that the proteins encoded by the tumor suppressor fat gene, the neurogenic slit gene and crumbs gene of Drosophila contain domains homologous with modules identified previously in laminin A. These proteins of Drosophila have a number of features in common: they have large extracellular regions containing l...

journal_title：FEBS letters

authors： Patthy L

更新日期：1992-02-24 00:00:00

abstract：:Apoptosis represents a fundamental process during fetal/post-natal testis development. Therefore pro- and anti-apoptotic proteins are essential to regulate testis physiology. c-Flip(L) is a known inhibitor of caspase 8/10 activity; in this study its perinatal expression in mouse male germ cells was investigated. In te...

journal_title：FEBS letters

authors： Giampietri C,Petrungaro S,Coluccia P,Antonangeli F,Paone A,Padula F,De Cesaris P,Ziparo E,Filippini A

更新日期：2006-11-13 00:00:00

abstract：:Chromatin phospholipidic fraction, as previously demonstrated, shows the same localization as RNA inside the nuclei. DNase and RNase treatment of nuclei removed almost totally the DNA, 63% of RNA and caused a 50% loss of phospholipids. The aim of the present investigation is to study the fraction of RNase undigested n...

journal_title：FEBS letters

authors： Micheli M,Albi E,Leray C,Magni MV

更新日期：1998-07-24 00:00:00

abstract：:To address biophysical principles and lipid interactions that underlie the properties of membrane proteins, modifications that vary the neighbors of tryptophan residues in the highly dynamic transmembrane helix of GW4,20 ALP23 (acetyl-GGAW4 A(LA)6 LAW20 AGA-amide) were examined using deuterium NMR spectroscopy. It was...

journal_title：FEBS letters

authors： McKay MJ,Greathouse DV,Koeppe RE 2nd

更新日期：2020-12-01 00:00:00

abstract：:Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...

journal_title：FEBS letters

authors： Sandberg K,Markwick AJ,Trinh DP,Catt KJ

更新日期：1988-12-05 00:00:00

abstract：:Changes in the ADP-ribosylation of total proteins and purified histones of rat liver nuclei after phenobarbital treatment (80 mg/kg, 24 h) have been studied. The [32P]NAD incorporation into total trichloroacetic acid precipitated proteins, in histone Hl and in core histones was evaluated, the specific radioactivities ...

journal_title：FEBS letters

authors： Bràz J,Lechner MC

更新日期：1986-04-21 00:00:00

abstract：:Treatment of either crude or purified preparations of the gamma-aminobutyrate (GABA)/benzodiazepine receptor complex with arginine-specific reagents resulted in a time- and concentration-dependent loss of [3H]muscimol binding activity. Following exposure to either 2,3-butanedione or phenylglyoxal (less than or equal t...

journal_title：FEBS letters

authors： Widdows KB,Kirkness EF,Turner AJ

更新日期：1987-09-28 00:00:00

abstract：:Bacterial RNA polymerase (RNAP) serves as a primase during replication of single-stranded plasmids and filamentous phages. Primer RNA (prRNA) synthesis from the origin regions of these replicons depends on the σ factor that normally participates in promoter recognition. However, it was proposed that σ may not be requi...

journal_title：FEBS letters

authors： Esyunina D,Pupov D,Kulbachinskiy A

更新日期：2019-02-01 00:00:00

abstract：:Trace amounts of gangliosides were found in rabbit skeletal muscle sarcoplasmic reticulum and their main part was shown, by high performance thin layer chromatography. to be GM3. Addition of GM3 to the soybean phospholipids used for reconstitution of proteoliposomes markedly increased ATP hydrolysis as well as Ca2+ up...

journal_title：FEBS letters

authors： Wang LH,Tu YP,Yang XY,Tsui ZC,Yang FY

更新日期：1996-06-17 00:00:00

abstract：:We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

journal_title：FEBS letters

authors： Schrempp S,Bayan N,Shechter E

更新日期：1994-12-12 00:00:00

abstract：:Selective protein degradation is an energy-dependent process performed by high-molecular-weight proteases. The activity of proteolytic components of these enzymes is coupled to the ATPase activity of their regulatory subunits or domains. Here, we obtained the proteolytic domain of Escherichia coli protease Lon by clon...

journal_title：FEBS letters

authors： Rasulova FS,Dergousova NI,Starkova NN,Melnikov EE,Rumsh LD,Ginodman LM,Rotanova TV

更新日期：1998-08-07 00:00:00

abstract：:PKD-mediated phosphorylation of class IIa HDACs frees the MEF2 transcription factor to activate genes that govern muscle differentiation and growth. Studies of the regulation and function of this signaling axis have involved MC1568 and Gö-6976, which are small molecule inhibitors of class IIa HDAC and PKD catalytic ac...

journal_title：FEBS letters

authors： Lemon DD,Harrison BC,Horn TR,Stratton MS,Ferguson BS,Wempe MF,McKinsey TA

更新日期：2015-04-28 00:00:00