A structure-activity study of fatty acid interaction with mitochondrial uncoupling protein.

abstract：:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...

journal_title：FEBS letters

authors： Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl D

更新日期：2012-06-04 00:00:00

abstract：:The Rho-guanine nucleotide exchange factors (Rho-GEFs) remodel the actin cytoskeleton via their Rho-GTPase targets and affect numerous physiological processes such as transformation and cell motility. They are therefore attractive targets to design specific inhibitors that may have therapeutic applications. Trio conta...

journal_title：FEBS letters

authors： Schmidt S,Diriong S,Méry J,Fabbrizio E,Debant A

更新日期：2002-07-17 00:00:00

abstract：:The p53 tumour suppressor protein is tightly controlled by the E3 ubiquitin ligase, mouse double minute 2 (MDM2), but maintains MDM2 expression as part of a negative feedback loop. We have identified the immunophilin, 25kDa FK506-binding protein (FKBP25), previously shown to be regulated by p53-mediated repression, as...

journal_title：FEBS letters

authors： Ochocka AM,Kampanis P,Nicol S,Allende-Vega N,Cox M,Marcar L,Milne D,Fuller-Pace F,Meek D

更新日期：2009-02-18 00:00:00

abstract：:The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

journal_title：FEBS letters

authors： Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

更新日期：1995-10-16 00:00:00

abstract：:Drug carriers such as liposomes are not readily transported across cellular barriers that constitute epithelia. However, certain epithelia (rabbit yolk sac endoderm and enterocytes of suckling rat gut proximal small intestine) are well known to transcytose maternal IgG by Fc receptor-mediated endocytic events. We have...

journal_title：FEBS letters

authors： Patel HM,Wild AE

更新日期：1988-07-18 00:00:00

abstract：:The archaeal exosome, a protein complex responsible for phosphorolytic degradation and tailing of RNA, has an RNA-binding platform containing Rrp4, Csl4, and DnaG. Aiming to detect novel interaction partners of the exosome, we copurified Nop5, which is a part of an rRNA methylating ribonucleoprotein complex, with the ...

journal_title：FEBS letters

authors： Gauernack AS,Lassek C,Hou L,Dzieciolowski J,Evguenieva-Hackenberg E,Klug G

更新日期：2017-12-01 00:00:00

abstract：:Myotonic dystrophy protein kinase (DMPK) and Rho-kinase are related. An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly. MYPT1 was phosphorylated by...

journal_title：FEBS letters

authors： Murányi A,Zhang R,Liu F,Hirano K,Ito M,Epstein HF,Hartshorne DJ

更新日期：2001-03-30 00:00:00

abstract：:Molecular dynamics analyses were performed to examine conformational changes in the C-domain of calmodulin and the N-domain of troponin C induced by binding of Ca(2+) ions. Analyses of conformational changes in calmodulin and troponin C indicated that the shortening of the distance between Ca(2+) ions and Ca(2+) bindi...

journal_title：FEBS letters

authors： Goto K,Toyama A,Takeuchi H,Takayama K,Saito T,Iwamoto M,Yeh JZ,Narahashi T

更新日期：2004-03-12 00:00:00

abstract：:Three of four natural compounds, which are caffeic acid, eupatilin and 4'-demethyleupatilin, isolated from Chinese plant, Artemisia rubripes Nakai selectively inhibited 5-lipoxygenase of cultured mastocytoma cells. Half-inhibition doses (ID50) for caffeic acid, eupatilin and 4'-demethyleupatilin were 3.7, 14 and 18 X ...

journal_title：FEBS letters

authors： Koshihara Y,Neichi T,Murota S,Lao A,Fujimoto Y,Tatsuno T

更新日期：1983-07-11 00:00:00

abstract：:The KlICL1 gene, encoding isocitrate lyase in Kluyveromyces lactis, is essential for ethanol utilization. Deletion analyses identified two functional promoter elements, CSRE-A and CSRE-B. Transcription is activated on ethanol, but not on glucose, glycerol or lactate. Expression depends on the KlCat8p transcription fac...

journal_title：FEBS letters

authors： Rodicio R,López ML,Cuadrado S,Cid AF,Redruello B,Moreno F,Heinisch JJ,Hegewald AK,Breunig KD

更新日期：2008-03-05 00:00:00

abstract：:The minimal functional enzyme unit of Na,K-ATPase consists of an alpha-beta complex. The alpha-subunit bears all functional domains of the enzyme and so far a regulatory role for the beta-subunit in the catalytic cycle has not been established. On the other hand, increasing experimental evidence suggests that the beta...

journal_title：FEBS letters

authors： Geering K

更新日期：1991-07-22 00:00:00

abstract：:We have recently cloned a cDNA encoding sucrose:fructan 6-fructosyltransferase (6-SFT), a key enzyme of fructan synthesis forming the beta-2,6 linkages typical of the grass fructans, graminans and phleins [Sprenger et al. (1995) Proc. Natl. Acad. Sci. USA 92, 11652-11656]. Here we report functional expression of 6-SFT...

journal_title：FEBS letters

authors： Sprenger N,Schellenbaum L,van Dun K,Boller T,Wiemken A

更新日期：1997-01-06 00:00:00

abstract：:The NAD-dependent glycerol-3-phosphate dehydrogenase (glycerol-3-phosphate:NAD+ oxidoreductase; EC 1.1.1.8; G3P DHG) was purified 178-fold to homogeneity from Saccharomyces cerevisiae strain H44-3D by affinity- and ion-exchange chromatography. SDS-PAGE indicated that the enzyme had a molecular mass of approximately 42...

journal_title：FEBS letters

authors： Albertyn J,van Tonder A,Prior BA

更新日期：1992-08-17 00:00:00

abstract：:Periplasmic adaptor proteins are essential components of bacterial tripartite multidrug efflux pumps. Here we report the 2.35 Å resolution crystal structure of the BesA adaptor from the spirochete Borrelia burgdorferi solved using selenomethionine derivatized protein. BesA shows the archetypal linear, flexible, multi-...

journal_title：FEBS letters

authors： Greene NP,Hinchliffe P,Crow A,Ababou A,Hughes C,Koronakis V

更新日期：2013-09-17 00:00:00

abstract：:Protein synthesis in cell-free systems of rat liver and kidney decreases markedly with age. Examination of activity changes of the different steps revealed for both types of organs that reduced binding of aminoacyl-tRNA to ribosomes and reduced peptidyl transfer might be of major importance for the decrease in overall...

journal_title：FEBS letters

authors： Gabius HJ,Engelhardt R,Deerberg F,Cramer F

更新日期：1983-08-22 00:00:00

abstract：:We examined the expression of peroxisome proliferator-activated receptors (PPARs) and the role of PPARs in cytokine production in mouse bone marrow-derived mast cells (mBMMCs). mBMMCs expressed PPARbeta strongly and gamma slightly, but not alpha. Activation of mBMMCs with antigen or calcium ionophore resulted in the i...

journal_title：FEBS letters

authors： Sugiyama H,Nonaka T,Kishimoto T,Komoriya K,Tsuji K,Nakahata T

更新日期：2000-02-11 00:00:00

abstract：:When the eicosapentaenoic acid (EPA)-deficient mutant strain IK-1Delta8 of the marine EPA-producing Shewanella marinintestina IK-1 was treated with various concentrations of hydrogen peroxide (H(2)O(2)), its colony-forming ability decreased more than that of the wild type. Protein carbonylation, induced by treating ce...

journal_title：FEBS letters

authors： Nishida T,Morita N,Yano Y,Orikasa Y,Okuyama H

更新日期：2007-09-04 00:00:00

abstract：:Human hepatocellular carcinoma (HCC) is one of the most common tumors worldwide, in which the genetic mechanisms of oncogenesis are still unclear. To investigate whether the genomic DNA copy number alterations may contribute to primary HCC, the cDNA microarray-based comparative genomic hybridization (CGH) analysis was...

journal_title：FEBS letters

authors： Huang J,Sheng HH,Shen T,Hu YJ,Xiao HS,Zhang Q,Zhang QH,Han ZG

更新日期：2006-06-26 00:00:00

abstract：:All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme respons...

journal_title：FEBS letters

authors： Sahin U,Blobel CP

更新日期：2007-01-09 00:00:00

abstract：:In plants exposed to high irradiances of visible light, the D1 protein in the reaction center of photosystem II is oxidatively damaged and rapidly degraded. Earlier work in our laboratory showed that the serine protease Deg2 performs the primary cleavage of photodamaged D1 protein in vitro. Here, we demonstrate that t...

journal_title：FEBS letters

authors： Huesgen PF,Schuhmann H,Adamska I

更新日期：2006-12-22 00:00:00

abstract：:Mitochondria in primary living hepatocytes were visualized in cells transfected with a chimeric plasmid encoding for the green fluorescent protein (GFP) of Aequorea victoria engineered to be specifically targeted to mitochondria, as described recently (Rizutto et al. (1995) Curr. Biol. 5, 635-642). The identification ...

journal_title：FEBS letters

authors： Venerando R,Miotto G,Pizzo P,Rizzuto R,Siliprandi N

更新日期：1996-03-18 00:00:00

abstract：:The cDNA coding for the inducible H+/hexose cotransporter of Chlorella kessleri has been cloned and sequenced. It was isolated by differential screening of a cDNA library prepared from glucose-induced cells. The increase in expression of the gene correlates quantitatively with the increase in uptake activity due to in...

journal_title：FEBS letters

authors： Sauer N,Tanner W

更新日期：1989-12-18 00:00:00

abstract：:Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

journal_title：FEBS letters

authors： Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

更新日期：2007-01-09 00:00:00

abstract：:The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs...

journal_title：FEBS letters

authors： Imperi F,Visca P

更新日期：2013-11-01 00:00:00

abstract：:PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...

journal_title：FEBS letters

authors： Gupta N,Nicholson DW,Ford-Hutchinson AW

更新日期：1999-04-16 00:00:00

abstract：:Putrescine N-methyltransferase (PMT) catalyses S-adenosylmethionine (SAM)-dependent methylation of putrescine in tropane alkaloid biosynthesis. PMT presumably evolved from the ubiquitous spermidine synthase (SPDS). SPDS protein structure suggested that only few amino acid exchanges in the active site were necessary to...

journal_title：FEBS letters

authors： Biastoff S,Reinhardt N,Reva V,Brandt W,Dräger B

更新日期：2009-10-20 00:00:00

abstract：:32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...

journal_title：FEBS letters

authors： Bork P

更新日期：1989-10-23 00:00:00

abstract：:Magainins are a family of antimicrobial peptides present in the skin extracts of Xenopus laevis. Both magainin-1 and -2 do not have any significant effect on the activity of protein kinase C (PKC). Magainin-2 was found to be readily phosphorylated by PKC to 0.5 mol 32P/mol of peptide. Neither magainin-1, which has a s...

journal_title：FEBS letters

authors： Nakabayashi H,Brown JH,Morell JL,Chen HC,Huang KP

更新日期：1990-07-02 00:00:00

abstract：:Mycobacterium tuberculosis contains multiple versions of the accA and accD genes that encode the alpha- and beta-subunits of at least three distinct multi-functional acyl-CoA carboxylase complexes. Because of its proposed involvement in pathogenic M. tuberculosis survival, the high-resolution crystal structure of the ...

journal_title：FEBS letters

authors： Holton SJ,King-Scott S,Nasser Eddine A,Kaufmann SH,Wilmanns M

更新日期：2006-12-22 00:00:00

abstract：:Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging po...

journal_title：FEBS letters

authors： Marques SA,Loureiro AP,Gomes OF,Garcia CC,Di Mascio P,Medeiros MH

更新日期：2004-02-27 00:00:00