The hexose carrier from Chlorella. cDNA cloning of a eucaryotic H+-cotransporter.

abstract：:The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/...

journal_title：FEBS letters

authors： Balzarini J,de Clercq E,Ayusawa D,Seno T

更新日期：1984-07-23 00:00:00

abstract：:The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non-equilibrium distribution, leading to enhanced emission from the directly excited low-energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735nm (F713 and ...

journal_title：FEBS letters

authors： Zucchelli G,Morosinotto T,Garlaschi FM,Bassi R,Jennings RC

更新日期：2005-04-11 00:00:00

abstract：:We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was obs...

journal_title：FEBS letters

authors： Kaether C,Gerdes HH

更新日期：1995-08-07 00:00:00

abstract：:Myogenesis is conducted by transcription factors including MyoD and myogenin. Myogenin is known to be polyubiquitinated by SCF (Skp1/Cullin 1/F-box protein) followed by proteasomal degradation, though the participating F-box protein is remaining unidentified. In this study, we found that myogenin in differentiated myo...

journal_title：FEBS letters

authors： Jogo M,Shiraishi S,Tamura TA

更新日期：2009-09-03 00:00:00

abstract：:The photophobic receptor (psRII) and its transducer pHtrII from Natronobacterium pharaonis were heterologously coexpressed in the phototaxis-deficient Halobacterium salinarium strain Pho81/w which lacks all four bacterial rhodopsins, i.e. the two ion pumps bacteriorhodopsin and halorhodopsin as well as the two sensory...

journal_title：FEBS letters

authors： Lüttenberg B,Wolff EK,Engelhard M

更新日期：1998-04-10 00:00:00

abstract：:The gap junctional intercellular communication mediated by Cx43 plays indispensable roles in both germ line development and postnatal folliculogenesis. In this study, we focused on the effect of follicle-stimulating hormone (FSH) on the Cx43 protein in rat primary granulosa cells and found that FSH stimulation elevate...

journal_title：FEBS letters

authors： Yogo K,Ogawa T,Akiyama M,Ishida N,Takeya T

更新日期：2002-11-06 00:00:00

abstract：:We have shown previously that the skeletal muscle ryanodine receptor mRNA of approximately 16,000 nucleotides codes 5,037 amino acid residues constituting the calcium release channel in skeletal muscle. In this study, RNA blot hybridization analysis shows that the brain contains an RNA species with an estimated size o...

journal_title：FEBS letters

authors： Takeshima H,Nishimura S,Nishi M,Ikeda M,Sugimoto T

更新日期：1993-05-10 00:00:00

abstract：:Intravenous injections of 25.5 nmol rat amylin into fasted anesthetized rats caused a rapid increase in plasma lactate followed by an increase in plasma glucose; there was a transient fall in blood pressure. Subcutaneous injection of 25.5 nmol amylin also caused increases in lactate and glucose but did not change bloo...

journal_title：FEBS letters

authors： Young AA,Wang MW,Cooper GJ

更新日期：1991-10-07 00:00:00

abstract：:Based on imino proton exchange catalysis, base-pair lifetimes and apparent dissociation constants are reported on the complexes formed by bisintercalation of echinomycin at the CpG steps of the d(ACGTATACGT)2 and d(ACGTTAACGT)2 duplexes. The lifetimes of the four central A x T base pairs between two echinomycin bindin...

journal_title：FEBS letters

authors： Park JY,Lee JH,Choi BS

更新日期：1998-04-24 00:00:00

abstract：:Unlike mammalian lysosomal cysteine proteases, the trypanosomal cysteine protease cruzipain contains a 130-amino acid residue C-terminal domain, in addition to the catalytic domain, and it is stable at neutral pH. The endogenous (with C-terminal domain) and recombinant (without C-terminal domain) cruzipains exhibit si...

journal_title：FEBS letters

authors： Stoka V,Turk B,McKerrow JH,Björk I,Cazzulo JJ,Turk V

更新日期：2000-03-03 00:00:00

abstract：:Neuronal activity is often associated with changes in gene expression. By a two-dimensional cDNA-display system, restriction landmark cDNA scanning, we identified a novel gene whose expression in the hippocampus was up-regulated by kainate stimulation. The mRNA expression was detected only in brain and up-regulated by...

journal_title：FEBS letters

authors： Nakayama T,Yaoi T,Yasui M,Kuwajima G

更新日期：1998-05-22 00:00:00

abstract：:The flash-induced voltage response of halorhodopsin at high NaCl concentration comprises two main kinetic components. The first component with tau approximately 1 micros does not exceed 4% of the overall response amplitude and is probably associated with the formation of the L (hR520) intermediate. The second main com...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kalaidzidis YL,Kaulen AD

更新日期：1998-05-01 00:00:00

abstract：:Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as we...

journal_title：FEBS letters

authors： Callebaut I,Courvalin JC,Mornon JP

更新日期：1999-03-05 00:00:00

abstract：:Xanthine oxidase was decreased 2- to 10-fold in all examined rat hepatomas irrespective of the malignancy; growth rate and degrees of histological differentiation of the neoplasms. The affinity to substrate (KM=6-8 muM) and the pH optimum (8.0) of the liver and hepatoma enzymes were the same. The reprogramming of gene...

journal_title：FEBS letters

authors： Prajda N,Weber G

更新日期：1975-11-15 00:00:00

abstract：:PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...

journal_title：FEBS letters

authors： Wang Z,Takemori H,Halder SK,Nonaka Y,Okamoto M

更新日期：1999-06-18 00:00:00

abstract：:In washed anterior pituitary membranes, there is enough GTP to occupy Ns and therefore to obtain activation of adenylate cyclase by vasointestinal peptide. GTP concentrations needed to obtain adenylate cyclase inhibition by dopamine (above 5 X 10- M) stimulate the adenylate cyclase. The dopamine effect is a blockade o...

journal_title：FEBS letters

authors： Bockaert J,Sebben-Perez M

更新日期：1983-09-05 00:00:00

abstract：:Tumor necrosis factor receptor associated factor 6 (TRAF6) is an important signaling molecule involved in a diverse array of physiological processes. It has been proposed that TRAF6, a RING finger-containing protein, acts as a ubiquitin ligase (E3) and a target for Lys-63 linked polyubiquitination mediated by Ubc13-Ue...

journal_title：FEBS letters

authors： Wooff J,Pastushok L,Hanna M,Fu Y,Xiao W

更新日期：2004-05-21 00:00:00

abstract：:When isolated reaction centres of photosystem II are subjected to photoinhibitory illumination, a 41 kDa SDS-PAGE band is observed under all experimental conditions. The same band is also found, together with lower molecular weight fragments of the D1 protein, in whole thylakoids and in all PSII sub-particles investig...

journal_title：FEBS letters

authors： Barbato R,Friso G,Rigoni F,Frizzo A,Giacometti GM

更新日期：1992-09-07 00:00:00

abstract：:hsp25 is a small, growth-related, mammalian stress protein which is highly accumulated in the stationary phase of Ehrlich ascites tumor in vivo. Ehrlich ascites cells cultivated in vitro under conditions of continuous exponential growth express hsp25 only at a low level. These cells were stably transfected with an euk...

journal_title：FEBS letters

authors： Knauf U,Bielka H,Gaestel M

更新日期：1992-09-14 00:00:00

abstract：:Using the polymerase chain reaction (PCR), we identified a gene family including more than 60 members which encoded similar G protein-coupled seven-transmembrane receptors. Sequence analyses of six representatives out of the 60 PCR clones showed that they had significant structural similarity to olfactory and optic re...

journal_title：FEBS letters

authors： Abe K,Kusakabe Y,Tanemura K,Emori Y,Arai S

更新日期：1993-02-01 00:00:00

abstract：:Helicobacter pylori secretes a unique virulence factor, Tipalpha, that enters gastric cells and both stimulates the production of the TNF-alpha and activates the NF-kappaB pathway. The structures of a truncated version of Tipalpha (TipalphaN34) in two crystal forms are presented here. Tipalpha adopts a novel beta(1)al...

journal_title：FEBS letters

authors： Tosi T,Cioci G,Jouravleva K,Dian C,Terradot L

更新日期：2009-05-19 00:00:00

abstract：:To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...

journal_title：FEBS letters

authors： Hagiwara Y,Nureki O,Tateno M

更新日期：2009-02-18 00:00:00

abstract：:The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e. TOCSY) experiments have been conducted for both the deuterated and natural-abundance samples. Using a 54 ms mixing time correlation peaks can be seen for all four...

journal_title：FEBS letters

authors： LeMaster DM

更新日期：1988-06-20 00:00:00

abstract：:The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

journal_title：FEBS letters

authors： Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

更新日期：1991-05-20 00:00:00

abstract：:gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a m...

journal_title：FEBS letters

authors： Touqui L,Jandrot-Perrus M,Vargaftig BB

更新日期：1989-09-25 00:00:00

abstract：:The nitration of tyrosine residues in protein occurs through the action of reactive oxygen and nitrogen species and is considered a marker of oxidative stress under pathological conditions. The most active nitrating species so far identified is peroxynitrite, the product of the reaction between nitric oxide and supero...

journal_title：FEBS letters

authors： Mallozzi C,Di Stasi AM,Minetti M

更新日期：2001-08-17 00:00:00

abstract：:High-molecular-weight [14C]hyaluronate was incubated with cultured fibroblasts from human uterine cervix and skin, and then the depolymerization of the hyaluronate was investigated. [14C]Hyaluronate in the medium of skin fibroblasts was depolymerized into a constant molecular weight (M(r) about 40,000), whereas that o...

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Ikeya H,Higuchi T,Tanaka A,Morikawa A,Saito Y,Takagaki K,Endo M

更新日期：1994-06-20 00:00:00

abstract：:The in vitro methylation of the +6, +24, and -215 located 5'-CCGG-3' sequences in the late E2A promoter of adenovirus type 2 (Ad2) DNA abrogates promoter function. 5-Methyldeoxycytidine (5-mC) at positions +6 and +24 in both or either of the two DNA complements in the late E2A promoter abolishes the formation of a hig...

journal_title：FEBS letters

authors： Hermann R,Doerfler W

更新日期：1991-04-09 00:00:00

abstract：:Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [14C]sialic acid or [3H]sphingosine, were tested, not only GD1a and GM3 but also GM2 and GM1, both of wh...

journal_title：FEBS letters

authors： Miyagi T,Tsuiki S

更新日期：1986-10-06 00:00:00

abstract：:Incubation of human thrombocytes in the presence of [3H]palmitic acid leads to incorporation of this fatty acid into the alpha subunit of Gs as described [Linder et al., Proc. Natl. Acad. Sci. USA 90 (1993) 3675-3679; Degtyarev et al., Biochemistry 32 (1993) 8057-8061] but also into the catalyst of adenylyl cyclase wh...

journal_title：FEBS letters

authors： Mollner S,Beck K,Pfeuffer T

更新日期：1995-09-11 00:00:00