Structural diversity in the six-fold redundant set of acyl-CoA carboxyltransferases in Mycobacterium tuberculosis.


:Mycobacterium tuberculosis contains multiple versions of the accA and accD genes that encode the alpha- and beta-subunits of at least three distinct multi-functional acyl-CoA carboxylase complexes. Because of its proposed involvement in pathogenic M. tuberculosis survival, the high-resolution crystal structure of the beta-subunit gene accD5 product has been determined and reveals a hexameric 356 kDa complex. Analysis of the active site properties of AccD5 and homology models of the other five M. tuberculosis AccD homologues reveals unexpected differences in their surface composition, providing a molecular rational key for a sorting mechanism governing correct acyl-CoA carboxylase holo complex assembly in M. tuberculosis.




FEBS letters


Holton SJ,King-Scott S,Nasser Eddine A,Kaufmann SH,Wilmanns M




Has Abstract


2006-12-22 00:00:00














  • A novel plant nuclear gene encoding chloroplast ribosomal protein S9 has a transit peptide related to that of rice chloroplast ribosomal protein L12.

    abstract::We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

    更新日期:1999-05-07 00:00:00

  • The verprolin family of proteins: regulators of cell morphogenesis and endocytosis.

    abstract::The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Aspenström P

    更新日期:2005-10-10 00:00:00

  • Selective inhibition of lignoceroyl-CoA synthetase by adenosine 5'-alkylphosphates.

    abstract::Structural analogs of adenosine 5'-acylphosphates, which are intermediates of the reaction catalyzed by acyl-CoA synthetases, were synthesized by condensing primary alcohols with AMP to examine the inhibitory effects on the lignoceroyl-CoA and palmitoyl-CoA synthetase activities. Hexadecyl, octadecyl, eicosyl, docosyl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shiraishi T,Tezuka K,Uda Y

    更新日期:1994-10-03 00:00:00

  • Characterization of a new membrane-bound cytochrome c of Rhodopseudomonas capsulata.

    abstract::A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c2 from the same bacterium did not cross...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hüdig H,Drews G

    更新日期:1983-02-21 00:00:00

  • Co-evolution of base composition and codon usage in Xenopus laevis and human globin genes with long-range DNA organization of their genome.

    abstract::Eucaryotic DNA is punctuated by many A+T-rich segments that we named A+T-rich linkers. Two types of these A+T-rich linkers can be distinguished: (i) isolated A+T-rich linkers, and (ii) A+T-rich linkers crowded in clusters. We have analysed the distribution of A+T-rich linker across the alpha- and beta-globin gene doma...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Moreau J,Scherrer K

    更新日期:1987-08-31 00:00:00

  • Trypanosomatid hydrogen peroxide [corrected] metabolism.

    abstract::The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Penketh PG,Kennedy WP,Patton CL,Sartorelli AC

    更新日期:1987-09-14 00:00:00

  • Breakdown and synthesis of polyphosphoinositides in fMetLeuPhe-stimulated neutrophils.

    abstract::The interconversions of the inositol-containing lipids (PI, PI-P and PI-P2) and their products (DG, inositol phosphates and PA) in human and rabbit neutrophils stimulated with fMetLeuPhe and PMA have been examined. PMA causes only the phosphorylation of PI to PI-P whereas fMetLeuPhe causes phosphorylation of both PI a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cockcroft S,Barrowman MM,Gomperts BD

    更新日期:1985-02-25 00:00:00

  • Characterization of membrane-associated prothrombin activator in normal and injured murine tissues.

    abstract::Thrombin is a multifunctional enzyme involved in coagulation, cell modulation and inflammation. We recently reported a novel membrane-associated prothrombin activator, abbreviated as MAPA, found in cultured fibroblasts and glial cell lines. In this study, we examined the physiological role of this enzyme. MAPA-like ac...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shikamoto Y,Shibusawa S,Okuyama I,Morita T

    更新日期:1997-08-04 00:00:00

  • Protective effect of acetyl-L-carnitine against oxidative stress induced by antiretroviral drugs.

    abstract::Both HIV infection per se and antiretroviral drugs might contribute to oxidative stress and mitochondrial dysfunctions. In this study we assess zidovudine, stavudine and didanosine on U937 and CEM cell lines. All these drugs induced apoptosis and increased intracellular hydrogen peroxide but not superoxide anions. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ferraresi R,Troiano L,Roat E,Nemes E,Lugli E,Nasi M,Pinti M,Calvani M,Iannuccelli M,Cossarizza A

    更新日期:2006-12-11 00:00:00

  • Inhibition of epidermal growth factor receptor functions by tyrosine kinase inhibitors in NIH3T3 cells.

    abstract::Epidermal growth factor (EGF) induces transformed phenotypes in EGF receptor-overexpressing NIH3T3 (ER12) cells. Tyrosine kinase inhibitors such as erbstatin and its stable analogue methyl 2,5-dihydroxycinnamate inhibited the EGF-induced phenotypic changes in these cells; while 5'-O-methylerbstatin, an inactive analog...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Umezawa K,Sugata D,Yamashita K,Johtoh N,Shibuya M

    更新日期:1992-12-21 00:00:00

  • Arachidonic acid induces specific membrane permeability increase in heart mitochondria.

    abstract::Micromolar concentrations of arachidonic acid cause in Ca2+ loaded heart mitochondria matrix swelling and Ca2+ release. These effects appear to be unrelated to the classical membrane permeability transition (MPT), as they are CsA insensitive, membrane potential independent and can also be activated by Sr2+. Atractylos...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Di Paola M,Zaccagnino P,Oliveros-Celis C,Lorusso M

    更新日期:2006-02-06 00:00:00

  • Intrinsic tryptophan phosphorescence as a marker of conformation and oxygen diffusion in purified cytochrome oxidase.

    abstract::Cytochrome oxidase exhibits phosphorescence from tryptophan in aqueous solution in the absence of oxygen. The lifetime for the resting reduced enzyme suspended in Tween-20 is around 30 ms at pH 8. The lifetime is longest between pH 7 and 8 and decreases with lowering of pH. Oxygen quenches the phosphorescence with a S...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Papp S,King TE,Vanderkooi JM

    更新日期:1991-05-20 00:00:00

  • Protein kinase inhibitors block the stimulation of the AMP-activated protein kinase by 5-amino-4-imidazolecarboxamide riboside.

    abstract::The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fryer LG,Parbu-Patel A,Carling D

    更新日期:2002-11-06 00:00:00

  • Human testis cDNA for the regulatory subunit RII alpha of cAMP-dependent protein kinase encodes an alternate amino-terminal region.

    abstract::Phosphorylations catalyzed by cAMP-dependent protein kinase are essential for sperm motility, and type II cAMP-dependent protein kinase in mature sperm has been shown to be firmly bound to the flagellum via the regulatory subunit, RII. The present study documents high-levelled expression of a human, testis-specific RI...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oyen O,Myklebust F,Scott JD,Hansson V,Jahnsen T

    更新日期:1989-03-27 00:00:00

  • Voltage-gated proton (H(v)1) channels, a singular voltage sensing domain.

    abstract::The main role of voltage-gated proton channels (Hv1) is to extrude protons from the intracellular milieu when, mediated by different cellular processes, the H(+) concentration increases. Hv1 are exquisitely selective for protons and their structure is homologous to the voltage sensing domain (VSD) of other voltage-gat...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Castillo K,Pupo A,Baez-Nieto D,Contreras GF,Morera FJ,Neely A,Latorre R,Gonzalez C

    更新日期:2015-11-14 00:00:00

  • Isolation and characterization of neuropeptide Y from porcine intestine.

    abstract::The isolation and primary structure of intestinal neuropeptide Y (NPY) is described. The peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for NPY. The amino acid sequence of this peptide is: Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala- Arg-Ty...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tatemoto K,Siimesmaa S,Jörnvall H,Allen JM,Polak JM,Bloom SR,Mutt V

    更新日期:1985-01-01 00:00:00

  • Ascorbic acid accumulation in plated human neutrophils.

    abstract::Ascorbic acid uptake was investigated in isolated, plated human neutrophils using high-performance liquid chromatography with coulometric electrochemical detection. Freshly isolated neutrophils contained 1.3 mM ascorbic acid and accumulated significantly greater amounts when physiologic concentrations of the vitamin w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Washko P,Rotrosen D,Levine M

    更新日期:1990-01-15 00:00:00

  • Unusual kinetic behavior predicted for alpha-keto acid dehydrogenase complexes.

    abstract::A novel regulation type, which may be observed as an unusual kinetic 'cooperativity', is predicted for the alpha-keto acid dehydrogenase complexes. The inter-relationship of this regulation with the well-known regulatory effect of enzyme phosphorylation is discussed. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Goldstein BN,Selivanov VA

    更新日期:1993-03-22 00:00:00

  • Discovery of the catalytic function of a putative 2-oxoacid dehydrogenase multienzyme complex in the thermophilic archaeon Thermoplasma acidophilum.

    abstract::Those aerobic archaea whose genomes have been sequenced possess a single 4-gene operon that, by sequence comparisons with Bacteria and Eukarya, appears to encode the three component enzymes of a 2-oxoacid dehydrogenase multienzyme complex. However, no catalytic activity of any such complex has ever been detected in th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Heath C,Jeffries AC,Hough DW,Danson MJ

    更新日期:2004-11-19 00:00:00

  • Mitochondrial uncoupling protein 2 gene transcript levels are elevated in maturating erythroid cells.

    abstract::Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Flachs P,Sponarova J,Kopecky P,Horvath O,Sediva A,Nibbelink M,Casteilla L,Medrikova D,Neckar J,Kolar F,Kopecky J

    更新日期:2007-03-20 00:00:00

  • Involvement of electrostatic phenomena in phospholamban-induced stimulation of Ca uptake into cardiac sarcoplasmic reticulum.

    abstract::The activity of the Ca-pumping ATPase of cardiac sarcoplasmic reticulum (SR) is controlled by the phosphorylation of the intrinsic regulatory protein phospholamban (PLB), which affects both the apparent Km(Ca) and the Vmax of the transport process. We have investigated the correlation between phosphorylation of PLB an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chiesi M,Schwaller R

    更新日期:1989-02-13 00:00:00

  • Cardiac to cancer: connecting connexins to clinical opportunity.

    abstract::Gap junctions and their connexin components are indispensable in mediating the cellular coordination required for tissue and organ homeostasis. The critical nature of their existence mandates a connection to disease while at the same time offering therapeutic potential. Therapeutic intervention may be offered through ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Grek CL,Rhett JM,Ghatnekar GS

    更新日期:2014-04-17 00:00:00

  • Identification of chemical compounds regulating PD-L1 by introducing HiBiT-tagged cells.

    abstract::Programmed death-ligand 1 (PD-L1) is a co-inhibitory molecule expressed on tumor cells. Immune checkpoint inhibitors focusing on the PD-L1 mechanism are now being studied for the treatment of various cancer types. However, the regulatory mechanism of PD-L1 is yet to be fully clarified, and a high-throughput system for...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Uchida Y,Matsushima T,Kurimoto R,Chiba T,Inutani Y,Asahara H

    更新日期:2021-01-09 00:00:00

  • A mutational analysis of active site residues in trans-3-chloroacrylic acid dehalogenase.

    abstract::trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-haloacrylates to yield malonate semialdehyde by a mechanism utilizing βPro-1, αArg-8, αArg-11, and αGlu-52. These residues are implicated in a promiscuous hydratase activity where 2-oxo-3-pentynoate is processed to acetop...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Poelarends GJ,Serrano H,Huddleston JP,Johnson WH Jr,Whitman CP

    更新日期:2013-09-02 00:00:00

  • The roles of organic anion permeases in aluminium resistance and mineral nutrition.

    abstract::Soluble aluminium (Al(3+)) is the major constraint to plant growth on acid soils. Plants have evolved mechanisms to tolerate Al(3+) and one type of mechanism relies on the efflux of organic anions that protect roots by chelating the Al(3+). Al(3+) resistance genes of several species have now been isolated and found to...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Delhaize E,Gruber BD,Ryan PR

    更新日期:2007-05-25 00:00:00

  • Fluorescent differential display: arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer.

    abstract::We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ito T,Kito K,Adati N,Mitsui Y,Hagiwara H,Sakaki Y

    更新日期:1994-09-05 00:00:00

  • The phosphoinositide 3-kinase/Akt pathway is activated by daunorubicin in human acute myeloid leukemia cell lines.

    abstract::Daunorubicin induces apoptosis in myeloid leukemia cells by activation of neutral sphingomyelinase and ceramide generation occurring 4-10 min after daunorubicin addition. We show here that daunorubicin is able to increase the phosphoinositide 3-kinase activity and enhance intracellular phosphoinositide 3-kinase lipid ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Plo I,Bettaïeb A,Payrastre B,Mansat-De Mas V,Bordier C,Rousse A,Kowalski-Chauvel A,Laurent G,Lautier D

    更新日期:1999-06-11 00:00:00

  • Expression of ATP binding cassette-transporter ABCG1 prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.

    abstract::Oxysterols result from cholesterol by enzymatic or oxidative processes. Some exert cytotoxic effects leading to necrosis or apoptosis. Detoxification of these compounds mainly occurs in the liver and requires transport from peripheral tissues towards it. Some ATP-binding cassette transporters are involved in export of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Engel T,Kannenberg F,Fobker M,Nofer JR,Bode G,Lueken A,Assmann G,Seedorf U

    更新日期:2007-04-17 00:00:00

  • Chemical synthesis and biological activity of a novel antibacterial peptide deduced from a pig myeloid cDNA.

    abstract::Several myeloid precursors of antibacterial peptides have recently been shown to share homologous pre- and pro-regions. Taking advantage of this homology, a novel cDNA was cloned from pig bone marrow RNA. This encodes a 166-residue polypeptide with highly conserved pre- (29 residues) and pro- (101 residues) sequences,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Storici P,Scocchi M,Tossi A,Gennaro R,Zanetti M

    更新日期:1994-01-17 00:00:00

  • Chemical grafting of functional NAD in the active site of a dehydrogenase: regeneration in situ.

    abstract::A functional NAD molecule was immobilized at the active site of Alcohol dehydrogenase within a proteic membrane. The presence and the functionality of the cofactor was checked by fluorescence analysis. The dehydrogenase NAD membrane does not require addition of soluble cofactor for its activity. The system represents ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Legoy MD,le Moullec JM,Thomas D

    更新日期:1978-10-15 00:00:00