Identification of the regions involved in DNA binding by the mouse PEBP2alpha protein.

abstract：:Iron regulatory protein 2 (IRP2) is a critical switch for cellular and systemic iron homeostasis. In iron-deficient or hypoxic cells, IRP2 binds to mRNAs containing iron responsive elements (IREs) and regulates their expression. Iron promotes proteasomal degradation of IRP2 via the F-box protein FBXL5. Here, we explor...

journal_title：FEBS letters

authors： Hausmann A,Lee J,Pantopoulos K

更新日期：2011-02-18 00:00:00

abstract：:Cellulysin, a crude cellulase from the plant parasitic fungus Trichoderma viride, induces the biosynthesis of volatiles in higher plants (Nicotiana plumbaginifolia, Phaseolus lunatus, and Zea mays) when applied to cut petioles by the transpiration stream. The pattern of the emitted volatiles largely resembles that fro...

journal_title：FEBS letters

authors： Piel J,Atzorn R,Gäbler R,Kühnemann F,Boland W

更新日期：1997-10-20 00:00:00

abstract：:Highly selective affinity labeling of the primer site of E. coli DNA polymerase I was performed with the 5'-reactive derivatives of oligothymidylate in the presence of poly(dA) template. Subtilysine cleavage proved that the site of affinity modification belonged to the 'Klenow' part of DNA polymerase I. If taken separ...

journal_title：FEBS letters

authors： Mitina RL,Mustaev AA,Zaychikov EF,Khomov VV,Lavrik OI

更新日期：1990-10-15 00:00:00

abstract：:The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...

journal_title：FEBS letters

authors： Kotelnikova RA,Kotelnikov AI,Bogdanov GN,Romanova VS,Kuleshova EF,Parnes ZN,Vol'pin ME

更新日期：1996-07-01 00:00:00

abstract：:The spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is an important viral structural protein. Based on bioinformatics analysis, 10 antigenic peptides derived from the S protein sequence were selected and synthesized. The antigenicity and immunoreactivity of all the peptides were tested in...

journal_title：FEBS letters

authors： Lu W,Wu XD,Shi MD,Yang RF,He YY,Bian C,Shi TL,Yang S,Zhu XL,Jiang WH,Li YX,Yan LC,Ji YY,Lin Y,Lin GM,Tian L,Wang J,Wang HX,Xie YH,Pei G,Wu JR,Sun B

更新日期：2005-04-11 00:00:00

abstract：:The potent inhibitors of protein kinase C (PKC), H7, staurosporine, and staurosporine derivatives, were examined for their inhibitory effects on novel PKC (nPKC) isozymes delta and epsilon. H7 and staurosporine, usually used as selective inhibitors of PKC, showed similar inhibitory effects on cPKC (a mixture of cPKC a...

journal_title：FEBS letters

authors： Mizuno K,Saido TC,Ohno S,Tamaoki T,Suzuki K

更新日期：1993-09-13 00:00:00

abstract：:Polysulfide is a bound sulfur species derived from endogenous H2S. When mouse neuroblastoma, Neuro2A cells were exposed to tert-butyl hydroperoxide after treatment with polysulfide, a significant decline in cell toxicity was observed. Rapid uptake of polysulfides induced translocation of Nrf2 into the nucleus, resulti...

journal_title：FEBS letters

authors： Koike S,Ogasawara Y,Shibuya N,Kimura H,Ishii K

更新日期：2013-11-01 00:00:00

abstract：:We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units ...

journal_title：FEBS letters

authors： Di Primo C,Rudloff I,Reigadas S,Arzumanov AA,Gait MJ,Toulmé JJ

更新日期：2007-02-20 00:00:00

abstract：:The human gene frataxin and its yeast homolog YFH1 affect mitochondrial function. Deficits in frataxin result in Friedreich ataxia, while deletion of YFH1 results in respiratory incompetence. We determined that as long as respiratory incompetent yeast express Yfh1p they do not accumulate excessive mitochondrial iron. ...

journal_title：FEBS letters

authors： Chen OS,Kaplan J

更新日期：2001-11-30 00:00:00

abstract：:The N-terminus of MDM2 proto-oncoprotein interacts with p53 and down modulates p53 activity by inhibiting transcriptional activity and promoting p53 degradation. MDMX is structurally related to MDM2 and also binds to p53. However, the function of MDMX has not been clarified yet. We found that MDM2 hetero-oligomerized ...

journal_title：FEBS letters

authors： Tanimura S,Ohtsuka S,Mitsui K,Shirouzu K,Yoshimura A,Ohtsubo M

更新日期：1999-03-19 00:00:00

abstract：:Persistent tumour necrosis factor alpha (TNF-alpha) exposure uncouples proximal T-cell receptor (TCR)-signalling events. Here, we demonstrate that chronic TNF-alpha exposure also attenuates signalling distal to the TCR, by specifically inhibiting Ca2+ influx evoked by thapsigargin in CD4+ T-cells. Mitogen-induced Ca2+...

journal_title：FEBS letters

authors： Church LD,Goodall JE,Rider DA,Bacon PA,Young SP

更新日期：2005-02-28 00:00:00

abstract：:In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brai...

journal_title：FEBS letters

authors： Seebahn A,Rose M,Enz R

更新日期：2008-07-09 00:00:00

abstract：:The cDNA coding for a novel member of the cysteine-rich protein family was isolated from a rat brain cDNA library. It encodes a protein, denoted cysteine-rich protein 2 (CRP2), of 208 amino acid residues containing two tandem repeats of an unusual LIM/double zinc-finger-like motif. The ubiquitous tissue distribution a...

journal_title：FEBS letters

authors： Okano I,Yamamoto T,Kaji A,Kimura T,Mizuno K,Nakamura T

更新日期：1993-10-25 00:00:00

abstract：:Neuritic plaques are the key pathological feature of Alzheimer's disease, and amyloid beta (Abeta) peptides are major component of these plaques. In this study, we demonstrated the influence of aluminum (Al) on the Abeta peptide degradation by cathepsin D. Al did not directly affect the cathepsin D activity using smal...

journal_title：FEBS letters

authors： Sakamoto T,Saito H,Ishii K,Takahashi H,Tanabe S,Ogasawara Y

更新日期：2006-12-11 00:00:00

abstract：:Using specific monoclonal and polyclonal antibodies, we have analyzed protein kinase C alpha and beta isozyme expression in human T cells from peripheral blood (PB) and from thymus. While the PKC beta isozyme was present in all T cell sub-types isolated from both PB and thymus, the alpha isozyme was found only in sing...

journal_title：FEBS letters

authors： Tarantino N,Debré P,Körner M

更新日期：1994-02-07 00:00:00

abstract：:A new secondary structure model for parts of the 7 SL RNA is proposed which indicates for a stretch of at least 40 bases a strong structural homology to the ribosomal protein L5 binding site of eukaryotic 5 S rRNA. It is suggested that the 5 S rRNA-like structural part of 7 SL RNA mediates binding of the signal recogn...

journal_title：FEBS letters

authors： Boehm S

更新日期：1987-02-09 00:00:00

abstract：:We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histon...

journal_title：FEBS letters

authors： Okuwaki M,Matsumoto K,Tsujimoto M,Nagata K

更新日期：2001-10-12 00:00:00

abstract：:We propose a unified framework for the identification of functional interaction structures of biomolecular networks in a way that leads to a new experimental design procedure. In developing our approach, we have built upon previous work. Thus we begin by pointing out some of the restrictions associated with existing s...

journal_title：FEBS letters

authors： Cho KH,Choo SM,Wellstead P,Wolkenhauer O

更新日期：2005-08-15 00:00:00

abstract：:Stimulation of human platelets increases cytoplasmic pH (pHi) via activation of Na+/H+ exchange. We have determined the effect of inhibiting Na+/H+ exchange on (i) thrombin-induced Ca2+ mobilization and (ii) turnover of 32P-labelled phospholipids. Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethyliso...

journal_title：FEBS letters

authors： Siffert W,Siffert G,Scheid P,Riemens T,Gorter G,Akkerman JW

更新日期：1987-02-09 00:00:00

abstract：:The Ala-30 and Asn-32 residues involved in the major antiparallel beta-sheet structure of human epidermal growth factor (hEGF) were substituted with various amino acid residues, and the receptor-binding affinities of the nine variant hEGFs were determined by the use of human KB cells. The Ala-30----Arg, Ala-30----His ...

journal_title：FEBS letters

authors： Koide H,Muto Y,Kasai H,Hoshi K,Takusari H,Kohri K,Takahashi S,Sasaki T,Tsukumo K,Miyake T

更新日期：1992-05-04 00:00:00

abstract：:In plant organelle genomes, homeologous recombination between heteroallelic positions of repetitive sequences is increased by dysfunction of the gene encoding MutS homolog 1 (MSH1), a plant organelle-specific homolog of bacterial mismatch-binding protein MutS1. The C-terminal region of plant MSH1 contains the GIY-YIG ...

journal_title：FEBS letters

authors： Fukui K,Harada A,Wakamatsu T,Minobe A,Ohshita K,Ashiuchi M,Yano T

更新日期：2018-12-01 00:00:00

abstract：:The effect of oligosaccharide carrying lipids on membrane fluidity has been investigated. Gangliosides GM1 and GQ1 were reconstituted into phosphatidylcholine bilayer membranes at low concentrations (less than 5 mol%). A strong fluidizing effect was observed leading to a suppression of the phase transition temperature...

journal_title：FEBS letters

authors： Ollmann M,Galla HJ

更新日期：1985-01-01 00:00:00

abstract：:Membrane incorporation and aggregation of the peptide alamethicin have been investigated as a function of lipid type. Head group and acyl chain regions both contribute to modulate alamethicin incorporation. Specifically, the peptide prefers thin membranes and saturated chains; incorporation is reduced by the presence ...

journal_title：FEBS letters

authors： Stankowski S,Schwarz G

更新日期：1989-07-03 00:00:00

abstract：:The effect of the three platelet-derived growth factor (PDGF) isoforms AA, AB and BB on the induction of the early growth response genes c-fos, egr-1 and c-myc mRNA in vascular smooth muscle cells from rat was compared with their respective mitogenic potency. The three PDGF isoforms strongly stimulated the induction t...

journal_title：FEBS letters

authors： Sachinidis A,Schulte K,Ko Y,Meyer zu Brickwedde MK,Hoppe V,Hoppe J,Vetter H

更新日期：1993-03-22 00:00:00

abstract：:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

journal_title：FEBS letters

authors： Colloc'h N,Prangé T

更新日期：2014-05-02 00:00:00

abstract：:In hepatocytes from control rats, the ureogenic action of epinephrine is mainly mediated through alpha 1-adrenoceptors and the effect is independent of the presence of extracellular calcium. In hepatocytes from adrenalectomized rats, both alpha 1- and beta-adrenoceptors are involved in the action of epinephrine. Furth...

journal_title：FEBS letters

authors： Hernández-Sotomayor SM,García-Sáinz JA

更新日期：1984-01-30 00:00:00

abstract：:Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...

journal_title：FEBS letters

authors： Rentsch D,Laloi M,Rouhara I,Schmelzer E,Delrot S,Frommer WB

更新日期：1995-08-21 00:00:00

abstract：:Sequence homology was found by computer analysis between potato spindle tuber viroid (PSTV) RNA and U3B snRNA of Novikoff hepatoma cells. This homology is colinear in arrangement, extends in length to 81% of the entire U3B snRNA molecule and is involved in the PSTV molecule unique sites which, if depicted in terms of ...

journal_title：FEBS letters

authors： Kiss T,Pósfai J,Solymosy F

更新日期：1983-11-14 00:00:00

abstract：:The manganese containing center of the oxygen evolving complex accumulates four oxidizing equivalents in the four stepped water oxidation cycle. Based on experiments on electrochromic absorption transients and the reduction rate of the primary electron donor, P680, it has been speculated that the oscillations of these...

journal_title：FEBS letters

authors： Haumann M,Bögershausen O,Junge W

更新日期：1994-11-21 00:00:00

abstract：:Acylphosphatase (AcP) activity in prokaryotes was classically attributed to some aspecific acid phosphatases. We identified an open reading frame for a putative AcP in the b0968 Escherichia coli gene and purified the recombinant enzyme after checking by RT-PCR that it was indeed expressed. EcoAcP has a predicted typic...

journal_title：FEBS letters

authors： Ramazzotti M,Parrini C,Stefani M,Manao G,Degl'Innocenti D

更新日期：2006-12-22 00:00:00