Abstract:
:We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units are located on the 3' side of the aptamer loop display an affinity for TAR below 1nM, i.e. one order of magnitude higher than the parent RNA aptamer. One of these combinations inhibits the TAR-dependent luciferase expression in a cell assay.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Di Primo C,Rudloff I,Reigadas S,Arzumanov AA,Gait MJ,Toulmé JJdoi
10.1016/j.febslet.2007.01.047subject
Has Abstractpub_date
2007-02-20 00:00:00pages
771-4issue
4eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00089-0journal_volume
581pub_type
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