Abstract:
:Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the beta-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosphorylation of muscarinic receptors, i.e. stimulation at lower concentrations and inhibition at higher concentrations. The stimulatory effect was reproduced with the beta gamma subunit of Go and the inhibitory effect with the combination of the alpha and beta gamma subunits.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Haga K,Haga Tdoi
10.1016/0014-5793(90)80968-osubject
Has Abstractpub_date
1990-07-30 00:00:00pages
43-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(90)80968-Ojournal_volume
268pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subun...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.090
更新日期:2004-09-24 00:00:00
abstract::Kinetic analyses of cpTat-mediated protein transport across the thylakoid membrane were conducted, revealing three important characteristics of this translocation pathway. First, transport via the cpTAT system displays a non-Michaelis-Menten, sigmoidal rate-substrate relationship with an apparent Hill coefficient of 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00231-x
更新日期:2003-04-10 00:00:00
abstract::Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80661-y
更新日期:1992-07-06 00:00:00
abstract::We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.048
更新日期:2006-05-29 00:00:00
abstract::Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03072-1
更新日期:2001-11-23 00:00:00
abstract::A tetradecapeptide with a sequence identical to residues 26-39 of the cro protein from bacteriophage lambda has been synthesized. This peptide has no secondary structure in an aqueous buffer but adopts an alpha-helical conformation in the presence of 20% hexafluoroisopropanol. The fluorescence of the single tyrosyl re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80638-3
更新日期:1983-03-21 00:00:00
abstract::Gp130 is the common signal transducing receptor subunit of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor and cardiotrophin-1. IL-6 and IL-11 induce gp130 homodimerization whereas the others lead to the formation of heterodimers with LIFR or OSMR. Binding e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01205-9
更新日期:2000-02-25 00:00:00
abstract::In humans, many responses to hypoxia including angiogenesis and erythropoiesis are mediated by the alpha/beta-heterodimeric transcription factor hypoxia inducible factor (HIF). The stability and/or activity of human HIF-1alpha are modulated by post-translational modifications including prolyl and asparaginyl hydroxyla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.012
更新日期:2006-04-03 00:00:00
abstract::The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80286-r
更新日期:1990-07-02 00:00:00
abstract::The purpose of the present study was to investigate whether 5-hydroxytryptamine (5-HT, serotonin) affects migration of vascular endothelial cells. 5-HT significantly enhanced migration of human aortic endothelial cells (HAECs), and this enhancement was completely inhibited by GR 55562, a 5-HT1 receptor antagonist, and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.10.068
更新日期:2005-12-19 00:00:00
abstract::γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12318
更新日期:2016-09-01 00:00:00
abstract::Telomerase is an almost universal cancer target that consists minimally of a core protein human telomerase reverse transcriptase (hTERT) and a RNA component human telomerase RNA (hTR). Some inhibitors of this enzyme are thought to function by the covalent binding to one or several cysteine residues; however, this inhi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12589
更新日期:2017-03-01 00:00:00
abstract::The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80107-8
更新日期:1988-07-18 00:00:00
abstract::Molecular dynamics simulations have been performed on the intrinsically disordered 39-residue N-terminal transactivation domain of p53 (p53(1-39)). Simulations not only revealed that p53(1-39) is natively compact, but also possesses a folded structure. Furthermore, leucine-rich hydrophobic clusters were found to play ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.060
更新日期:2009-02-04 00:00:00
abstract::The structure of rabbit liver Hsp90 was reevaluated by limited trypsinolysis, N-terminal sequencing and determination of the site that is phosphorylated by casein kinase II. Limited proteolysis results in formation of four groups of large peptides with M(r) in the range of 26-41 kDa. Peptides with M(r) 39-41 kDa were ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01056-x
更新日期:1999-09-03 00:00:00
abstract::We previously found a brain-specific glycoprotein in the rat brain. It postnatally increases and is rich in the mature brain. We cloned cDNA of this protein. It is composed of a signal peptide, a V-type immunoglobulin domain, two C1-type immunoglobulin domains, a transmembrane segment and a cytoplasmic region containi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00724-2
更新日期:1997-07-14 00:00:00
abstract::The presence of the hydroxy-aluminium polymer (Al13-(OH)24O4(H2O)12(7+)) was noticed inside synaptosomes when synaptosomes were incubated with Al(NO3)3 at neutral pH values. Dysprosium nitrate (Dy(NO3)3)--a shift reagent--facilities the identification of the Al13 species distinctly inside the synaptosomes. 27Al NMR wa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81364-r
更新日期:1992-10-12 00:00:00
abstract::Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid into prostanoids which are involved in apoptosis and inflammation. Two distinct COXs have been identified: COX-1 which is constitutively expressed and COX-2 which is induced by different products such as tumor promoters or growth factors. Pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02924-6
更新日期:2001-10-12 00:00:00
abstract::The glycine-alanine (GA) repeat of the Epstein-Barr virus nuclear antigen-1 inhibits in cis ubiquitin-dependent proteolysis in mammalian cells through a yet unknown mechanism. In the present study we demonstrate that the GA repeat targets an evolutionarily conserved step in proteolysis since it can prevent the degrada...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01296-1
更新日期:2003-12-04 00:00:00
abstract::A comparison is made between the PQA----P+QA- and PQAQB----P+QAQB-transitions in Rps. viridis and Rb. sphaeroides reaction centers (RCs) by the use of light-induced Fourier transform infrared (FTIR) difference spectroscopy. In Rb. sphaeroides RCs, we identify a signal at 1650 cm-1 which is present in the P+QA-minus-PQ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81506-j
更新日期:1990-06-18 00:00:00
abstract::A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80967-n
更新日期:1990-07-30 00:00:00
abstract::Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01777-9
更新日期:2000-01-21 00:00:00
abstract::A fusion gene was constructed consisting of heat labile enterotoxin chain B (LTB) of E. coli genetically linked at its C-terminus to the beta-subunit of human chorionic gonadotropin in translational fusion, under the control of tac promoter and LTB signal sequence. Expression of the fusion gene (about 5 microgram/ml) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00428-0
更新日期:1996-05-27 00:00:00
abstract::All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme respons...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.074
更新日期:2007-01-09 00:00:00
abstract::The Golgi apparatus is known to modify and sort newly synthesized secretory proteins. However, fundamental mysteries remain about the structure, operation, and dynamics of this organelle. Important insights have emerged from studying the Golgi in yeasts. For example, yeasts have provided direct evidence for Golgi cist...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.10.072
更新日期:2009-12-03 00:00:00
abstract::alpha2-Macroglobulin was derivatized with a sulfhydryl cross-linker and sandwiched between a mica substrate and a silicon nitride tip, both coated with gold, of an atomic force microscope and force curve measurement was carried out. An extensive downward deflection of the cantilever was observed in the retracting real...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00319-5
更新日期:1996-04-29 00:00:00
abstract::Signal recognition particle (SRP) interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the endoplasmic reticulum. Membrane targeting is mediated by the 68 and the 72 kDa proteins of SRP. We have cloned and sequenced cDNA encoding the 68 kDa protein of canine...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80518-n
更新日期:1990-12-10 00:00:00
abstract::Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80070-j
更新日期:1991-01-14 00:00:00
abstract::D-aspartate and human chorionic gonadotropin act synergistically to increase testosterone production in purified rat Leydig cells, and D-aspartate stimulates testosterone synthesis even in the absence of human chorionic gonadotropin stimulation. In addition, D-aspartate enhances steady-state cellular mRNA and protein ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00840-6
更新日期:1999-07-09 00:00:00
abstract::The primary structure of rhodopsin from the octopus Paroctopus defleini has been determined by parallel analysis of the protein and corresponding cDNA. The amino acid sequence is most similar to the recently cloned Drosophila opsins. Similarities to bovine and human opsins are also evident. The transmembrane topology ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80388-0
更新日期:1988-05-09 00:00:00