Abstract:
:A fusion gene was constructed consisting of heat labile enterotoxin chain B (LTB) of E. coli genetically linked at its C-terminus to the beta-subunit of human chorionic gonadotropin in translational fusion, under the control of tac promoter and LTB signal sequence. Expression of the fusion gene (about 5 microgram/ml) in E. coli was confirmed by immunoblot analysis using both anti-LTB and anti-betahCG polyclonal antibodies. The fusion protein was efficiently processed and exported to the periplasmic space. LTB in the fusion protein retained its ability to bind to GM1 ganglioside receptor. Mice immunized with the fusion protein produce antibodies that recognize recombinant betahCG and the native hCG suggesting its potential use as a contraceptive vaccine.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Pillai D,Dixit A,Alok D,Garg LCdoi
10.1016/0014-5793(96)00428-0subject
Has Abstractpub_date
1996-05-27 00:00:00pages
23-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(96)00428-0journal_volume
387pub_type
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