Abstract:
:CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within GM3-enriched plasma membrane domains of SupT1 cells and coimmunoprecipitation experiments revealed that CXCR4 was immunoprecipitated by IgG anti-GM3 after gp120 pretreatment. These findings reveal that gp120 binding induces a strict association between CXCR4 and ganglioside GM3, supporting the view that GM3 and CXCR4 are components of a functional multimolecular complex critical for HIV-1 entry.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sorice M,Garofalo T,Misasi R,Longo A,Mattei V,Sale P,Dolo V,Gradini R,Pavan Adoi
10.1016/s0014-5793(01)02830-7keywords:
subject
Has Abstractpub_date
2001-09-28 00:00:00pages
55-60issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(01)02830-7journal_volume
506pub_type
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