Pro-urokinase and prekallikrein are both associated with platelets. Implications for the intrinsic pathway of fibrinolysis and for therapeutic thrombolysis.


:The contact-dependent intrinsic pathway of fibrinolysis involving factor XII, prekallikrein (PK) and pro-urokinase (pro-UK) remains poorly understood. Casein autography of washed, intact platelets revealed both PK and pro-UK. Accordingly, platelets may mediate physiological thrombolysis by this pathway since factor XIIa activates PK and kallikrein activates pro-UK. Acid washing dissociated PK but not pro-UK from platelets. Exogenous pro-UK was specifically incorporated by platelets from the ambient fluid and similarly could not be dissociated from intact platelets. Therefore, platelets may also mediate an effect from therapeutically administered pro-UK by prolonging its half-life.




FEBS letters


Gurewich V,Johnstone M,Loza JP,Pannell R




Has Abstract


1993-03-08 00:00:00














  • Identification of a cDNA encoding a Drosophila calcium/calmodulin regulated protein phosphatase, which has its most abundant expression in the early embryo.

    abstract::A 3.3 kb cDNA encoding the complete amino acid sequence of a calcium/calmodulin regulated protein phosphatase has been isolated from a Drosophila eye disc cDNA library. The predicted protein of 560 amino acids (molecular mass 62 kDa) is 73-78% identical to human PP2B isoforms. The cDNA hybridised to the X-chromosome a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brown L,Chen MX,Cohen PT

    更新日期:1994-02-14 00:00:00

  • Cholesterol reverts Triton X-100 preferential solubilization of sphingomyelin over phosphatidylcholine: a 31P-NMR study.

    abstract::The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non-solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X-100. Quant...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahyayauch H,Collado MI,Goñi FM,Lichtenberg D

    更新日期:2009-09-03 00:00:00

  • EPR spectroscopic characterization of an 'iron only' nitrogenase. S = 3/2 spectrum of component 1 isolated from Rhodobacter capsulatus.

    abstract::The alternative nitrogenase of Rhodobacter capsulatus, isolated from a nifHDK deletion mutant, has been purified to near homogeneity and identified as an 'iron only' nitrogenase. The dithionite-reduced component 1 ('FeFe protein') of this enzyme showed an EPR spectrum consisting of two components: a minor S = 1/2 sign...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Müller A,Schneider K,Knüttel K,Hagen WR

    更新日期:1992-05-25 00:00:00

  • Ribosomal proteins S2, S6, S10, S14, S15 and S25 are localized on the surface of mammalian 40 S subunits and stabilize their conformation. A study with immobilized trypsin.

    abstract::Trypsin immobilized on collagen membranes has been used to digest accessible ribosomal proteins of rat liver 40 S subunits. Six proteins (S2, S6, S10, S14, S15 and S25) have been found to be highly exposed on the surface of 40 S particles. They appear to be in close physical contact and localized in the same region of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marion MJ,Marion C

    更新日期:1988-05-23 00:00:00

  • A 3D model of the voltage-dependent anion channel (VDAC).

    abstract::Eukaryotic porins are a group of membrane proteins whose best known role is to form an aqueous pore channel in the mitochondrial outer membrane. As opposed to the bacterial porins (a large family of protein whose 3D structure has been determined by X-ray diffraction), the structure of eukaryotic porins (also termed VD...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Casadio R,Jacoboni I,Messina A,De Pinto V

    更新日期:2002-06-05 00:00:00

  • Insulin activates protein kinase B, inhibits glycogen synthase kinase-3 and activates glycogen synthase by rapamycin-insensitive pathways in skeletal muscle and adipose tissue.

    abstract::Insulin stimulated protein kinase B alpha (PKB alpha) more than 10-fold and decreased glycogen synthase kinase-3 (GSK3) activity by 50 +/- 10% in skeletal muscle and adipocytes. Rapamycin did not prevent the activation of PKB, inhibition of GSK3 or stimulation of glycogen synthase up to 5 min. Thus rapamycin-insensiti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cross DA,Watt PW,Shaw M,van der Kaay J,Downes CP,Holder JC,Cohen P

    更新日期:1997-04-07 00:00:00

  • Dominant-negative effect of mutant valosin-containing protein in aggresome formation.

    abstract::Lewy bodies (LBs) are the pathologic hallmark of Parkinson's disease. Recent studies revealed that LBs exhibit several morphologic and molecular similarities to aggresomes. Aggresomes are perinuclear aggregates representing intracellular deposits of misfolded proteins. Recently, valosin-containing protein (VCP) was on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kitami MI,Kitami T,Nagahama M,Tagaya M,Hori S,Kakizuka A,Mizuno Y,Hattori N

    更新日期:2006-01-23 00:00:00

  • Impact of 9-(2-phosphonylmethoxyethyl)adenine on (deoxy)ribonucleotide metabolism and nucleic acid synthesis in tumor cells.

    abstract::Following exposure to 9-(2-phosphonylmethoxyethyl)adenine (an inhibitor of the cellular DNA polymerases alpha, delta and epsilon), human erythroleukemia K562, human T-lymphoid CEM and murine leukemia L1210 cells markedly accumulated in the S phase of the cell cycle. In contrast to DNA replication, RNA synthesis (trans...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hatse S,De Clercq E,Balzarini J

    更新日期:1999-02-19 00:00:00

  • Characterization of human presenilin 1 using N-terminal specific monoclonal antibodies: Evidence that Alzheimer mutations affect proteolytic processing.

    abstract::The majority of cases of early-onset familial Alzheimer disease are caused by mutations in the recently identified presenilin 1 (PS1) gene, located on chromosome 14. PS1, a 467 amino acid protein, is predicted to be an integral membrane protein containing seven putative transmembrane domains and a large hydrophilic lo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mercken M,Takahashi H,Honda T,Sato K,Murayama M,Nakazato Y,Noguchi K,Imahori K,Takashima A

    更新日期:1996-07-08 00:00:00

  • Divergent effect of proteasome inhibition on interleukin-1beta and tumor necrosis factor alpha signaling in human astroglial cells.

    abstract::Impaired functioning of the proteasome pathway is one of the molecular mechanism underlying neurodegenerative changes in Alzheimer's disease. In this study, we report that dysfunction of the proteasome pathway in astroglial cells leads to decreased survival and dysregulation of chemokines by differential regulation of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Choi K,Lee J,Choi C

    更新日期:2007-10-02 00:00:00

  • Shear stress-induced shedding of soluble intercellular adhesion molecule-1 from saphenous vein endothelium.

    abstract::Within 6 h, shear stress upregulated intercellular adhesion molecule-1 (ICAM-1) (two- to four-fold, P<0.001) and induced matrix metalloproteinase-2 (MMP-2) in cultured human saphenous vein endothelial cells. By 8 h endothelial ICAM-1 levels returned to baseline, with concomitant increase in soluble ICAM-1 (sICAM-1) (P...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sultan S,Gosling M,Nagase H,Powell JT

    更新日期:2004-04-23 00:00:00

  • Diversity and similarity in the tRNA world: overall view and case study on malaria-related tRNAs.

    abstract::Transfer RNAs (tRNAs) are ancient macromolecules that have evolved under various environmental pressures as adaptors in translation in all forms of life but also towards alternative structures and functions. The present knowledge on both "canonical" and "deviating" signature motifs retrieved from vertical and horizont...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Pütz J,Giegé R,Florentz C

    更新日期:2010-01-21 00:00:00

  • Cooperative repression of cyclin-dependent kinase inhibitor p21 gene expression by hepatitis B virus X protein and hepatitis C virus core protein.

    abstract::Co-infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) is common and is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma (HCC). Here, we demonstrated that HBV X protein (HBx) and HCV core protein additively repress the universal cyclin-de...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Han HJ,Jung EY,Lee WJ,Jang KL

    更新日期:2002-05-08 00:00:00

  • Rabbit skeletal muscle myosin. Unfolded carboxyl-terminus and its role in molecular assembly.

    abstract::We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maeda K,Rösch A,Maéda Y,Kalbitzer HR,Wittinghofer A

    更新日期:1991-04-09 00:00:00

  • mRNA 5' ends targeted by cytoplasmic recapping cluster at CAGE tags and select transcripts are alternatively spliced.

    abstract::Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5' rapid amplification of cDNA ends (RACE) shows that nearly all uncapped ends correspond to...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Berger MR,Alvarado R,Kiss DL

    更新日期:2019-04-01 00:00:00

  • Low-temperature microRNA expression in the painted turtle, Chrysemys picta during freezing stress.

    abstract::Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Biggar KK,Storey KB

    更新日期:2015-11-30 00:00:00

  • MDR-1 gene expression is a minor factor in determining the multidrug resistance phenotype of MCF7/ADR and KB-V1 cells.

    abstract::The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim HM,Oh GT,Hong DH,Kim MS,Kang JS,Park SM,Han SB

    更新日期:1997-07-21 00:00:00

  • PP1/PP2A phosphatases inhibitors okadaic acid and calyculin A block ERK5 activation by growth factors and oxidative stress.

    abstract::Okadaic acid is an inhibitor of the protein Ser/Thr phosphatases PP1 and PP2A, which blocks the activation of extracellular signal-regulated protein kinase 5 (ERK5), a member of the MAP kinase family activated by growth factors and several types of stressors. The blocking of ERK5 activation by okadaic acid was observe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Garcia L,Garcia F,Llorens F,Unzeta M,Itarte E,Gómez N

    更新日期:2002-07-17 00:00:00

  • The involvement of photosystem II-generated H2O2 in photoinhibition.

    abstract::The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibito...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bradley RL,Long KM,Frasch WD

    更新日期:1991-07-29 00:00:00

  • Lysine-87 is a functionally important residue in human prothymosin alpha.

    abstract::Human prothymosin alpha mutants were generated with the aid of random mutagenesis and screened for their ability to inhibit yeast Saccharomyces cerevisiae cell growth. Conversion of Lys-87 to Glu resulted in an inactivated prothymosin alpha mutant, which lost the ability of the wild-type protein to block yeast cell gr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rubtsov Y,Vartapetian A

    更新日期:1996-11-18 00:00:00

  • Cyclin A-Cys41 does not undergo cell cycle-dependent degradation in Xenopus extracts.

    abstract::Truncated cyclin A and cyclin B lacking the N-terminal domain comprising the 'destruction box' escape from proteolysis and arrest cells at metaphase. Mutation of a conserved arginine residue of the destruction domain makes cyclin B resistant to proteolysis. Here we show that mutation of the same residue also makes cyc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lorca T,Devault A,Colas P,Van Loon A,Fesquet D,Lazaro JB,Dorée M

    更新日期:1992-07-13 00:00:00

  • Two non-vesicular ATP release pathways in the mouse erythrocyte membrane.

    abstract::Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Qiu F,Wang J,Spray DC,Scemes E,Dahl G

    更新日期:2011-11-04 00:00:00

  • Inhibition of Na,K-ATPase activity by cGMP is isoform-specific in brain endothelial cells.

    abstract::cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pontiggia L,Winterhalter K,Gloor SM

    更新日期:1998-10-09 00:00:00

  • The 20S proteasome gene family in Arabidopsis thaliana.

    abstract::The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Parmentier Y,Bouchez D,Fleck J,Genschik P

    更新日期:1997-10-27 00:00:00

  • A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs.

    abstract::A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

    更新日期:1993-03-08 00:00:00

  • The protein kinase C activator phorbol-12-myristate-13-acetate enhances cyclic AMP accumulation in pheochromocytoma cells.

    abstract::The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hollingsworth EB,Ukena D,Daly JW

    更新日期:1986-02-03 00:00:00

  • Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro.

    abstract::Previously, several studies have demonstrated that autophosphorylation of the C-terminal tyrosine residues (Tyr1316 and Tyr1322) affects the signaling properties of the insulin receptor in vivo. To assess the biochemical consequences of the C-terminal phosphorylation in vitro, we have constructed, purified and charact...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tennagels N,Bergschneider E,Al-Hasani H,Klein HW

    更新日期:2000-08-11 00:00:00

  • Photoreceptors and olfactory cells express the same retinal guanylyl cyclase isoform in medaka: visualization by promoter transgenics.

    abstract::We examined the spatial expression patterns of two orphan receptor guanylyl cyclase genes OlGC4 and OlGC5 during embryogenesis of medaka and characterized the 5' flanking region required for tissue-specific expression of OlGC4 by introducing promoter-GFP fusion constructs into medaka embryos. Expression of OlGC5 is co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kusakabe T,Suzuki N

    更新日期:2000-10-20 00:00:00

  • Homodimerization of coronin A through the C-terminal coiled-coil domain is essential for multicellular differentiation of Dictyostelium discoideum.

    abstract::Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

    更新日期:2020-04-16 00:00:00

  • Overexpression of poly(A)-binding protein down-regulates the translation or the abundance of its own mRNA.

    abstract::Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hornstein E,Harel H,Levy G,Meyuhas O

    更新日期:1999-08-27 00:00:00