Pro-urokinase and prekallikrein are both associated with platelets. Implications for the intrinsic pathway of fibrinolysis and for therapeutic thrombolysis.

abstract：:Branched-chain 2-oxoacid dehydrogenase complex has been purified to near homogeneity by a simple, rapid procedure. The final product contains endogenous kinase activity capable of phosphorylating and inactivating the complex. Phosphorylation continues after complete inactivation, indicating the possibility of several ...

journal_title：FEBS letters

authors： Lawson R,Cook KG,Yeaman SJ

更新日期：1983-06-27 00:00:00

abstract：:Prodigiosin 25-C inhibited the accumulation of 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange in the acidic compartments of baby hamster kidney cells with little perturbation of cellular ATP levels. In rat liver lysosomes, prodigiosin 25-C inhibited the proton pump activity with an IC50 of a...

journal_title：FEBS letters

authors： Kataoka T,Muroi M,Ohkuma S,Waritani T,Magae J,Takatsuki A,Kondo S,Yamasaki M,Nagai K

更新日期：1995-02-06 00:00:00

abstract：:The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one ...

journal_title：FEBS letters

authors： Kondo S,Kondo H,Nakane S,Kodaka T,Tokumura A,Waku K,Sugiura T

更新日期：1998-06-12 00:00:00

abstract：:A high-potential iron-sulfur cluster with characteristics similar to a Rieske-type center was detected in the plasma membrane of Sulfolobus acidocaldarius by EPR spectroscopy. In the reduced form this center has g-values of gz = 2.031, gy = 1.890 and gx = 1.725 (gav = 1.88, rhombicity = 0.37) and its reduction potenti...

journal_title：FEBS letters

authors： Anemüller S,Schmidt CL,Schäfer G,Teixeira M

更新日期：1993-02-22 00:00:00

abstract：:A PCR-based subtractive cloning procedure was used to identify genes expressed at higher levels in the pancreatic beta cell line betaTC1, as compared to the pancreatic alpha cell line alphaTC1. One of the clones isolated by this procedure corresponded to the regulatory subunit (RIalpha) of protein kinase A (PKA). Usin...

journal_title：FEBS letters

authors： Arava Y,Adamsky K,Belleli A,Shaltiel S,Walker MD

更新日期：1998-03-20 00:00:00

abstract：:Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...

journal_title：FEBS letters

authors： Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela K

更新日期：2006-03-06 00:00:00

abstract：:The mucosal epithelium secretes a variety of antimicrobial peptides that act as part of the innate immune system to protect against invading microbes. Here, we describe the functional properties of human defensin (HD) 5, the major antimicrobial peptide produced by Paneth cells in the ileum, in relation to its structur...

journal_title：FEBS letters

authors： de Leeuw E,Burks SR,Li X,Kao JP,Lu W

更新日期：2007-02-06 00:00:00

abstract：:The regulatory mechanism by which GATA-3 suppresses IFN-gamma gene expression was investigated. A reduction of GATA-3 using RNA interference technology enhanced, whereas overexpression of GATA-3 suppressed IFN-gamma mRNA expression. IL-4 expression was reciprocally affected by GATA-3. GATA-3-mediated down-regulation o...

journal_title：FEBS letters

authors： Kaminuma O,Kitamura F,Kitamura N,Miyagishi M,Taira K,Yamamoto K,Miura O,Miyatake S

更新日期：2004-07-16 00:00:00

abstract：:Activation of T-cells infected by HIV-1 results in activation of long terminal repeat (LTR)-dependent viral transcription and ultimately the production of infectious virus. Although full T-cell activation requires a complex series of intracellular signals, including protein kinase C activation, calcium mobilisation, a...

journal_title：FEBS letters

authors： Baldari CT,Macchia G,Massone A,Telford JL

更新日期：1992-06-15 00:00:00

abstract：:To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...

journal_title：FEBS letters

authors： Fujita-Yoshigaki J,Shirouzu M,Koide H,Nishimura S,Yokoyama S

更新日期：1991-12-09 00:00:00

abstract：:We have developed a high-throughput sequencing approach that enables us to determine terminal telomere sequences from tens of thousands of individual Schizosaccharomyces pombe telomeres. This method provides unprecedented coverage of telomeric sequence complexity in fission yeast. S. pombe telomeres are composed of mo...

journal_title：FEBS letters

authors： Bennett HW,Liu N,Hu Y,King MC

更新日期：2016-12-01 00:00:00

abstract：:We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

journal_title：FEBS letters

authors： Schrempp S,Bayan N,Shechter E

更新日期：1994-12-12 00:00:00

abstract：:Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to...

journal_title：FEBS letters

authors： Golderer G,Loidl P,Gröbner P

更新日期：1987-10-05 00:00:00

abstract：:Intracellular accumulation of anthracycline derivatives was measured in a human embryonic kidney cell line (HEK) and a resistant subline (HEK/multidrug resistance protein (MRP1)) overexpressing MRP1 at the plasma membrane surface. Two compounds (daunorubicin and doxorubicin) were rejected outside the multidrug-resista...

journal_title：FEBS letters

authors： Manciu L,Chang X,Riordan JR,Buyse F,Ruysschaert JM

更新日期：2001-03-23 00:00:00

abstract：:Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as we...

journal_title：FEBS letters

authors： Callebaut I,Courvalin JC,Mornon JP

更新日期：1999-03-05 00:00:00

abstract：:Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction ([NADH][acetaldehyde][H(+)])/([ethanol][NAD(+)]) remains above ...

journal_title：FEBS letters

authors： Kalnenieks U,Galinina N,Toma MM,Pickford JL,Rutkis R,Poole RK

更新日期：2006-09-18 00:00:00

abstract：:Fibroblast growth factor-2 (FGF-2) is mitogenic for the human breast cancer cell line MCF-7; here we investigate some of the signaling pathways subserving this activity. FGF-2 stimulation of MCF-7 cells resulted in a global increase of intracellular tyrosine phosphorylation of proteins, particularly FGF receptor subst...

journal_title：FEBS letters

authors： Vercoutter-Edouart A,Lemoine J,Smart CE,Nurcombe V,Boilly B,Peyrat J,Hondermarck H

更新日期：2000-08-04 00:00:00

abstract：:Recent studies indicate that junctophilin (JP) subtypes contribute to the formation of the junctional membrane complexes between the plasma membrane and the endoplasmic/sarcoplasmic reticulum (ER/SR) in excitable cells. Cardiac muscle contains the diad, in which the transverse (T) tubule of the invaginated cell membra...

journal_title：FEBS letters

authors： Komazaki S,Nishi M,Takeshima H

更新日期：2003-05-08 00:00:00

abstract：:The synthesis of gangliosides GM3 and GD3 is carried out by the successive addition of sialic acid residues on lactosylceramide (LacCer) by the Golgi located sialyltransferases Sial-T1 and Sial-T2, respectively. CHO-K1 cells lack Sial-T2 and only express GM3. Here we show that the activity of Sial-T1 was near 2.5-fold...

journal_title：FEBS letters

authors： Spessott W,Crespo PM,Daniotti JL,Maccioni HJ

更新日期：2012-07-30 00:00:00

abstract：:We screened active compounds from natural marine products able to increase PPARalpha/gamma transcriptional activity. Sargaquinoic acid (SQA) and sargahydroquinoic acid (SHQA) from Sargassum yezoense were identified as novel PPARalpha/gamma dual agonists. The binding affinity of SQA with PPARgamma was higher than that ...

journal_title：FEBS letters

authors： Kim SN,Choi HY,Lee W,Park GM,Shin WS,Kim YK

更新日期：2008-10-15 00:00:00

abstract：:Since the discovery of the new members of the UCP (uncoupling protein) family, UCP2 and UCP3, very few studies have dealt with the regulation of their expression. Bearing this in mind, administration of a single intravenous injection of TNF-alpha (100 microg/kg body weight) to rats resulted in a significant increase i...

journal_title：FEBS letters

authors： Busquets S,Sanchís D,Alvarez B,Ricquier D,López-Soriano FJ,Argilés JM

更新日期：1998-12-04 00:00:00

abstract：:Myotonic dystrophy protein kinase (DMPK) is a serine-threonine protein kinase encoded by the myotonic dystrophy (DM) locus on human chromosome 19q13.3. It is a close relative of other kinases that interact with members of the Rho family of small GTPases. We show here that the actin cytoskeleton-linked GTPase Rac-1 bin...

journal_title：FEBS letters

authors： Shimizu M,Wang W,Walch ET,Dunne PW,Epstein HF

更新日期：2000-06-23 00:00:00

abstract：:Exposing a macrophage-like murine cell line to copper-oxidised low-density lipoprotein led to DNA fragmentation which was inhibited by the putative Ca2+/Mg2+ endonuclease inhibitor, zinc sulphate. DNA fragmentation preceded loss of membrane impermeability. These results suggest that apoptosis may be a mechanism of mac...

journal_title：FEBS letters

authors： Reid VC,Hardwick SJ,Mitchinson MJ

更新日期：1993-10-18 00:00:00

abstract：:We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the ...

journal_title：FEBS letters

authors： Tsuchiya E,Hiraga K,Fukui S,Miyakawa T

更新日期：1989-07-03 00:00:00

abstract：:The two cytoskeletal proteins VASP and WASP and the protein Homer share a conserved domain, currently designated the WHI domain (WASP homology domain 1) or EVH1 domain (ENA/VASP homology domain 1), which could play an important role in various cellular events such as transport, folding of proteins, and signal transduc...

journal_title：FEBS letters

authors： Callebaut I,Cossart P,Dehoux P

更新日期：1998-12-18 00:00:00

abstract：:A photoactive reaction center complex was isolated from a thermophilic green sulfur bacterium, Chlorobium tepidum under anaerobic conditions. The electron transfer occurred from heme c to the photo-oxidized reaction center chlorophyll, P840+, with a half time (t1/2) of 110 or 340 microseconds at 24 or 12 degrees C, re...

journal_title：FEBS letters

authors： Oh-oka H,Kamei S,Matsubara H,Iwaki M,Itoh S

更新日期：1995-05-22 00:00:00

abstract：:Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...

journal_title：FEBS letters

authors： Matzura H

更新日期：1970-01-15 00:00:00

abstract：:Regulation of supra-macromolecular composition and catalytic activity of a heterodimeric enzyme, gamma-glutamyltransferase, in the system of Aerosol OT (sodium bis(2-ethylhexyl) sulfosuccinate) reversed micelles in octane were studied. Variation of the surfactant hydration degree (parameter, determining dimensions of ...

journal_title：FEBS letters

authors： Kabanov AV,Nametkin SN,Chernov NN,Klyachko NL,Levashov AV

更新日期：1991-12-16 00:00:00

abstract：:Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...

journal_title：FEBS letters

authors： Gaul G,Dutly F,Frei K,Foguet M,Lübbert H

更新日期：1992-08-03 00:00:00

abstract：:Polymerising proteins of the actin family are nearly ubiquitous. Crenactins, restricted to Crenarchaea, are more closely related to actin than bacterial MreB. Crenactins occur in gene clusters hinting at an unknown, but conserved function. We solved the crystal structure of crenactin at 3.2 Å resolution. The protein c...

journal_title：FEBS letters

authors： Izoré T,Duman R,Kureisaite-Ciziene D,Löwe J

更新日期：2014-03-03 00:00:00