Abstract:
:We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the presence of WGA-binding materials around the nuclear pore under an electron microscope. Using WGA-agarose column chromatography of yeast nuclear extracts, a novel Mg2+-dependent ATPase was isolated. Its activity was highly sensitive to WGA and stimulated by Nonidet P-40 or phosphatidylserine. We suggest that the WGA-sensitive ATPase plays a role in yeast nuclear transport of DNA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tsuchiya E,Hiraga K,Fukui S,Miyakawa Tdoi
10.1016/0014-5793(89)80739-2subject
Has Abstractpub_date
1989-07-03 00:00:00pages
285-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80739-2journal_volume
250pub_type
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