Abstract:
:We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4+ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol polyphosphate 5-phosphatase. Treatment of cells with up to 10 microg/ml gp120 from between 10 min and 24 h was without effect upon inositol phosphate turnover in both basal cells, and in C305 and OKT3 stimulated cells. This is the first report that biologically competent gp120 does not affect any aspect of inositol phosphate turnover in either basal or receptor-activated lymphocytes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sumner MT,Shears SBdoi
10.1016/s0014-5793(97)00880-6subject
Has Abstractpub_date
1997-08-11 00:00:00pages
75-80issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00880-6journal_volume
413pub_type
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