Abstract:
:Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. Consistently, knockdown of their target kinases (PKA, PKCδ, CaMKII and smMLCK) resulted in a decrease in mitochondrial ATP synthesis activity. Among them, mitochondria of smMLCK-knockdown cells contained only a small amount of ATP synthase, while the α- and β-subunits of ATP synthase were produced normally, suggesting that smMLCK affects assembly (or decay) of ATP synthase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sugawara K,Fujikawa M,Yoshida Mdoi
10.1016/j.febslet.2013.10.012subject
Has Abstractpub_date
2013-11-29 00:00:00pages
3843-7issue
23eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00769-2journal_volume
587pub_type
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