Expression of blood clotting factor VIII:C gene in capillary endothelial cells.

abstract：:Brain nitric oxide synthase is a Ca2+/calmodulin-regulated enzyme which converts L-arginine into NO. Enzymatic activity of this enzyme essentially depends on NADPH and is stimulated by tetrahydrobiopterin (H4biopterin). We found that purified NO synthase contains enzyme-bound H4biopterin, explaining the enzymatic acti...

journal_title：FEBS letters

authors： Mayer B,John M,Heinzel B,Werner ER,Wachter H,Schultz G,Böhme E

更新日期：1991-08-19 00:00:00

abstract：:Chemotherapy is a major anticancer therapeutic modality, however, multidrug resistance (MDR) is frequently observed and hinders treatment efficacy. Here, we investigated the role and potential mechanism of the long noncoding RNA (lncRNA) FENDRR in adriamycin resistance of chronic myeloid leukaemia (CML) cells. FENDRR ...

journal_title：FEBS letters

authors： Zhang F,Ni H,Li X,Liu H,Xi T,Zheng L

更新日期：2019-08-01 00:00:00

abstract：:Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)-gamma1 in cell mitogenesis. In this study, we introduced wild-type PLC-gamma1 plasmid and mutants Y771F and Y783F into CCL-39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771...

journal_title：FEBS letters

authors： Pei ZD,Williamson JR

更新日期：1998-02-13 00:00:00

abstract：:Erythrocytes are exceptionally suited for analysis of non-exocytotic release mechanisms of ATP, because these cells under physiological conditions lack vesicles. Previous studies have indicated, that Pannexin1 (Panx1) provides a key ATP permeation pathway in many cell types, including human and frog erythrocytes. Here...

journal_title：FEBS letters

authors： Qiu F,Wang J,Spray DC,Scemes E,Dahl G

更新日期：2011-11-04 00:00:00

abstract：:As a result of Myc-dependent transcription of the LDH-A gene, Myc-transformed cells (Rat1-Myc) exhibit increased lactate production rates (LPR) even under aerobic conditions (the Warburg effect). Recently, the increased susceptibility to stress-induced apoptosis associated with Myc transfection has been linked to the ...

journal_title：FEBS letters

authors： Papas KK,Sun L,Roos ES,Gounarides JS,Shapiro M,Nalin CM

更新日期：1999-03-12 00:00:00

abstract：:A recombinant antibody light chain (L chain) maintained under non-denaturing conditions displayed preferential cleavage of synthetic peptides conjugated to methylcoumarinamide (MCA) on the C-terminal side of Arg and Lys residues. The same L chain renatured from a denaturing solvent (guanidine hydrochloride) acquired t...

journal_title：FEBS letters

authors： Sun M,Paul S

更新日期：1997-05-05 00:00:00

abstract：:Vesicular stomatitis virus (VSV) is a prototypic virus commonly used in studies of endocytosis and membrane trafficking. One proposed mechanism for VSV entry involves initial fusion with internal vesicles of multivesicular endosomes followed by back-fusion of these vesicles into the cytoplasm. One feature of endosomal...

journal_title：FEBS letters

authors： Roth SL,Whittaker GR

更新日期：2011-03-23 00:00:00

abstract：:The α-subunit of the cardiac voltage-gated sodium channel (NaV1.5) plays a central role in cardiomyocyte excitability. We have recently reported that NaV1.5 is post-translationally modified by arginine methylation. Here, we aimed to identify the enzymes that methylate NaV1.5, and to describe the role of arginine methy...

journal_title：FEBS letters

authors： Beltran-Alvarez P,Espejo A,Schmauder R,Beltran C,Mrowka R,Linke T,Batlle M,Pérez-Villa F,Pérez GJ,Scornik FS,Benndorf K,Pagans S,Zimmer T,Brugada R

更新日期：2013-10-01 00:00:00

abstract：:This review focuses on the factors that regulate the proliferation of pancreatic islet beta-cells in vitro, and in particular on the intracellular pathways that convey the mitogenic signal into a proliferative response. Substances as diverse as nutrients, polypeptides, cytokines, adrenergic agents, lithium, phorbol es...

journal_title：FEBS letters

authors： Sjöholm A

更新日期：1992-10-19 00:00:00

abstract：:Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...

journal_title：FEBS letters

authors： Flachs P,Sponarova J,Kopecky P,Horvath O,Sediva A,Nibbelink M,Casteilla L,Medrikova D,Neckar J,Kolar F,Kopecky J

更新日期：2007-03-20 00:00:00

abstract：:Recombinant porcine growth hormone (rPGH) was solubilised from inclusion bodies (IB's) using either 6 M guanidinium hydrochloride (GnHCl), 7.5 M urea or by a novel method using a cationic surfactant, cetyltrimethylammonium chloride (CTAC). Circular dichroism (CD) analysis of the secondary (2 degrees) structure of the ...

journal_title：FEBS letters

authors： Puri NK,Cardamone M

更新日期：1992-07-06 00:00:00

abstract：:3-(4'-Methyl-1'-naphthyl)-propionic acid, 1',4'-endoperoxide (NEPO) provides singlet state of oxygen (1O2, 1delta g) at 37 degrees C in sodium phosphate buffer (pH 7.2), acetate buffer (pH 4.5), methanol or chloroform, through the retro-Diels-Alder reaction. The total amount of 1O2 generated by NEPO was calculated usi...

journal_title：FEBS letters

authors： Nakano M,Kambayashi Y,Tatsuzawa H,Komiyama T,Fujimori K

更新日期：1998-07-31 00:00:00

abstract：:Recent studies show LDL receptor-related protein 1B, LRP1B as a transducer of extracellular signals. Here, we identify six interacting partners of the LRP1B cytoplasmic region by yeast two-hybrid screen and confirmed their in vivo binding by immunoprecipitation. One of the partners, PICK1 recognizes the C-terminus of ...

journal_title：FEBS letters

authors： Shiroshima T,Oka C,Kawaichi M

更新日期：2009-01-05 00:00:00

abstract：:Both HIV infection per se and antiretroviral drugs might contribute to oxidative stress and mitochondrial dysfunctions. In this study we assess zidovudine, stavudine and didanosine on U937 and CEM cell lines. All these drugs induced apoptosis and increased intracellular hydrogen peroxide but not superoxide anions. The...

journal_title：FEBS letters

authors： Ferraresi R,Troiano L,Roat E,Nemes E,Lugli E,Nasi M,Pinti M,Calvani M,Iannuccelli M,Cossarizza A

更新日期：2006-12-11 00:00:00

abstract：:The tendency of DNA to form fibers upon condensation with counterions is reviewed. It is shown that chromatin fibers may acquire a relatively constant diameter of about 30 nm simply as an optimal size achieved upon neutralization of DNA, without requiring a repetitive internal structure. Thus the size of chromatin fib...

journal_title：FEBS letters

authors： Subirana JA

更新日期：1992-05-11 00:00:00

abstract：:Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fr...

journal_title：FEBS letters

authors： Froidevaux R,Krier F,Nedjar-Arroume N,Vercaigne-Marko D,Kosciarz E,Ruckebusch C,Dhulster P,Guillochon D

更新日期：2001-02-23 00:00:00

abstract：:Incorporation of dioleoyl N-(monomethoxy polyethyleneglycol succinyl)phosphatidylethanolamine (PEG-PE) into large unilamellar liposomes composed of egg phosphatidylcholine:cholesterol (1:1) does not significantly increase the content leakage when the liposomes are exposed to 90% human serum at 37 degrees C, yet the li...

journal_title：FEBS letters

authors： Klibanov AL,Maruyama K,Torchilin VP,Huang L

更新日期：1990-07-30 00:00:00

abstract：:Retinoic acid receptor-related orphan receptor-alpha (RORalpha) is a nuclear orphan receptor. Adenovirus-mediated overexpression of RORalpha1 and RORalpha4 suppressed tumor necrosis factor-alpha (TNF-alpha)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) ...

journal_title：FEBS letters

authors： Migita H,Satozawa N,Lin JH,Morser J,Kawai K

更新日期：2004-01-16 00:00:00

abstract：:Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....

journal_title：FEBS letters

authors： Hejgaard J,Jacobsen S,Svendsen I

更新日期：1991-10-07 00:00:00

abstract：:The complete amino acid sequence of human plasma apolipoprotein C-III (apoC-III) isolated from normal subjects is described. ApoC-III is a linear polypeptide chain of 79 amino acids. Tryptic digestion of intact apoC-III produced 5 major peptides, while tryptic digestion of the citraconylated protein yielded two peptid...

journal_title：FEBS letters

authors： Hospattankar AV,Brewer HB Jr,Ronan R,Fairwell T

更新日期：1986-03-03 00:00:00

abstract：:The lack of trehalose accumulation in most plant species has been partly attributed to the presence of an active trehalase. Although trehalose synthesis enzymes are thought to be cytosolic, and previous studies have indicated that trehalase activity is extracellular, the exact location of the enzyme has not yet been e...

journal_title：FEBS letters

authors： Frison M,Parrou JL,Guillaumot D,Masquelier D,François J,Chaumont F,Batoko H

更新日期：2007-08-21 00:00:00

abstract：:A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase gene family, designated GalNAc-T7, was cloned and expressed. GalNAc-T7 exhibited different properties compared to other characterized members of this gene family, in showing apparent exclusive specificity for partial...

journal_title：FEBS letters

authors： Bennett EP,Hassan H,Hollingsworth MA,Clausen H

更新日期：1999-10-29 00:00:00

abstract：:The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of th...

journal_title：FEBS letters

authors： Toutant M,Bockaert J,Homburger V,Rouot B

更新日期：1987-09-28 00:00:00

abstract：:The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine...

journal_title：FEBS letters

authors： van Heyningen S

更新日期：1983-11-28 00:00:00

abstract：:Small RNAs are non-coding regulatory RNA molecules that control gene expression by mediating mRNA degradation, translational inhibition, or chromatin modification. Virus-derived small RNAs induce silencing of viral RNAs and are essential for antiviral defense in both animal and plant systems. The role of host endogeno...

journal_title：FEBS letters

authors： Jin H

更新日期：2008-08-06 00:00:00

abstract：:Connexin 43 (Cx43) hemichannels may form open channels in the plasma membrane when exposed to specific stimuli, e.g. reduced extracellular concentration of divalent cations, and allow passage of fluorescent molecules and presumably a range of smaller physiologically relevant molecules. However, the permeability profil...

journal_title：FEBS letters

authors： Hansen DB,Braunstein TH,Nielsen MS,MacAulay N

更新日期：2014-04-17 00:00:00

abstract：:Plant G proteins of the ROP/RAC family regulate cellular processes including cytoskeletal rearrangement in polar growth. Activation of the ROP molecular switch is triggered by guanine nucleotide exchange factors. Plant-specific RopGEFs are exclusively active on ROPs despite their high homology to animal Rho proteins. ...

journal_title：FEBS letters

authors： Fricke I,Berken A

更新日期：2009-01-05 00:00:00

abstract：:cGMP has been shown to either activate or inhibit Na,K-ATPase activity. Using mouse brain endothelial cells which express both ouabain-resistant alpha1 and ouabain-sensitive alpha2 and alpha3 isoforms, we show that cGMP reduces total Na,KATPase activity to about 58%. The inhibition is prevented by the protein kinase G...

journal_title：FEBS letters

authors： Pontiggia L,Winterhalter K,Gloor SM

更新日期：1998-10-09 00:00:00

abstract：:The effect of the non-steroidal antioestrogens tamoxifen and toremifene on voltage-gated cationic currents was examined in primary cultures of rat hypothalamic neurones and the C1300 mouse neuroblastoma cell line using the whole-cell patch clamp technique. When applied to the external bathing solution both tamoxifen a...

journal_title：FEBS letters

authors： Hardy SP,deFelipe C,Valverde MA

更新日期：1998-09-04 00:00:00

abstract：:The prohormone convertase PC2 is one of the major subtilisin/kexin-like enzymes responsible for the formation of small bioactive peptides in neural and endocrine cells. This convertase is unique among the members of the subtilisin/kexin-like mammalian serine proteinase family in that it undergoes zymogen processing of...

journal_title：FEBS letters

authors： Benjannet S,Mamarbachi AM,Hamelin J,Savaria D,Munzer JS,Chrétien M,Seidah NG

更新日期：1998-05-22 00:00:00