RORalpha1 and RORalpha4 suppress TNF-alpha-induced VCAM-1 and ICAM-1 expression in human endothelial cells.

abstract：:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1...

journal_title：FEBS letters

authors： Hung F,Luo D,Sauvé DM,Muller MT,Roberge M

更新日期：1996-02-12 00:00:00

abstract：:In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation ...

journal_title：FEBS letters

authors： Lal SK,Kumar V

更新日期：1999-07-30 00:00:00

abstract：:A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...

journal_title：FEBS letters

authors： Selden C,Jones M,Wade D,Hodgson H

更新日期：1990-09-17 00:00:00

abstract：:Glucocorticoid hormones (GHs) regulate cell proliferation of neural progenitor cells (NPCs) contributing to reduction of neurogenesis after stress. We show here that dexamethasone (Dex) decreases BRUCE/Apollon (BRUCE) in cultured NPCs in a GH-receptor-dependent manner. Downregulation of BRUCE by Dex or using silencing...

journal_title：FEBS letters

authors： Sippel M,Rajala R,Korhonen L,Bornhauser B,Sokka AL,Naito M,Lindholm D

更新日期：2009-07-07 00:00:00

abstract：:Monocyte chemoattractant protein-1 (MCP-1) is a member of the beta chemokine family which acts through specific seven transmembrane receptors to recruit monocytes, basophils, and T lymphocytes to sites of inflammation. To identify regions of the human MCP-1 protein which are important for its biological activity, we h...

journal_title：FEBS letters

authors： Steitz SA,Hasegawa K,Chiang SL,Cobb RR,Castro MA,Lobl TJ,Yamada M,Lazarides E,Cardarelli PM

更新日期：1998-07-03 00:00:00

abstract：:A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from ...

journal_title：FEBS letters

authors： Prestle J,Hornung E,Schönfelder M,Mundry KW

更新日期：1992-02-10 00:00:00

abstract：:We tested the truncated 7-37 glucagon-like peptide 1 (TGLP-1), a naturally occurring porcine intestinal peptide, and other members of the glucagon family, including pancreatic glucagon (G-29), GLP-1 and GLP-2 for their ability to activate the cAMP generating system in rat gastric glands and HGT-1 human gastric cancer ...

journal_title：FEBS letters

authors： Hansen AB,Gespach CP,Rosselin GE,Holst JJ

更新日期：1988-08-15 00:00:00

abstract：:Genetic factors seem to play a significant role in susceptibility to systemic lupus erythematosus (SLE). We previously described the amino acid polymorphism (Val14Met) within the IFN-gamma receptor 1 (IFN-gammaRI), and that the frequency of the Metl4 allele in SLE patients was significantly higher than that of the hea...

journal_title：FEBS letters

authors： Nakashima H,Inoue H,Akahoshi M,Tanaka Y,Yamaoka K,Ogami E,Nagano S,Arinobu Y,Niiro H,Otsuka T,Niho Y

更新日期：1999-06-18 00:00:00

abstract：:5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20-100-fold weaker than the natur...

journal_title：FEBS letters

authors： Rossi AG,Thomas MJ,O'Flaherty JT

更新日期：1988-11-21 00:00:00

abstract：:Structural and biochemical studies have begun to elucidate the pathway of RNA silencing that leads to the formation of the RISC complex. The outstanding feature of this pathway is the precise recognition and processing of double-stranded RNA. We present a review of recent structures that illustrate the molecular mecha...

journal_title：FEBS letters

authors： Collins RE,Cheng X

更新日期：2005-10-31 00:00:00

abstract：:gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a m...

journal_title：FEBS letters

authors： Touqui L,Jandrot-Perrus M,Vargaftig BB

更新日期：1989-09-25 00:00:00

abstract：:It has been suggested that alpha-dystroglycan links the dystrophin-associated protein complex and extracellular matrix and that the absence of dystrophin and alpha-dystroglycan in Duchenne muscular dystrophy (DMD) may lead to the breakdown of this linkage. In the present study, myotubes from DMD patients and murine X-...

journal_title：FEBS letters

authors： Angoli D,Corona P,Baresi R,Mora M,Wanke E

更新日期：1997-05-26 00:00:00

abstract：:Disruption of the gene pgil of Saccharomyces cerevisiae, which codes for phosphoglucose isomerase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6-phosphate was much higher in mutant than in wild-type cells. Phosphorylase a activity and the s...

journal_title：FEBS letters

authors： Corominas J,Clotet J,Fernández-Bañares I,Boles E,Zimmermann FK,Guinovart JJ,Ariño J

更新日期：1992-09-28 00:00:00

abstract：:Hyperthermia such as that occurring during fever may improve cell survival during infection, although its mechanism of action is largely unknown. Here we show that acute exposure to mild, but not severe, heat shock induces the synthesis of cyclin D1 that plays a critical role(s) in G1 progression of the cell cycle. Th...

journal_title：FEBS letters

authors： Han SI,Oh SY,Jeon WJ,Kim JM,Lee JH,Chung HY,Choi YH,Yoo MA,Kim HD,Kang HS

更新日期：2002-03-27 00:00:00

abstract：:Guluronate and mannuronate oligomers with various degree of polymerization were prepared from polyguluronate (PG) and polymannuronate (PM) with an alginate lyase from a Pseudoalteromonas sp., and their activities to induce cytokine secretion from mouse macrophage cell line RAW264.7 cells were examined. Enzymatically d...

journal_title：FEBS letters

authors： Iwamoto M,Kurachi M,Nakashima T,Kim D,Yamaguchi K,Oda T,Iwamoto Y,Muramatsu T

更新日期：2005-08-15 00:00:00

abstract：:In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...

journal_title：FEBS letters

authors： Faulhammer HG,Joshi RL

更新日期：1987-06-15 00:00:00

abstract：:The ubiquitin-like modifier, FAT10, is involved in proteasomal degradation and antigen processing. As ubiquitin and the ubiquitin-like modifier, ISG15, cotranslationally modify proteins, we investigated whether FAT10 could also be conjugated to newly synthesized proteins. Indeed, we found that nascent proteins are mod...

journal_title：FEBS letters

authors： Spinnenhirn V,Bitzer A,Aichem A,Groettrup M

更新日期：2017-01-01 00:00:00

abstract：:FACS analysis together with PIPLC treatment was applied to PI-anchoring antigens such as DAF (decay-accerelating factor, CD55), 1F5 antigen (CD59), CD14 and CD16 on the cell surfaces of blood cells from a normal adult and a male patient with paroxysmal nocturnal hemoglubinuria (PNH). Through the extensive analysis, th...

journal_title：FEBS letters

authors： Taguchi R,Funahashi Y,Ikezawa H,Nakashima I

更新日期：1990-02-12 00:00:00

abstract：:Artificial spider silk proteins may form fibers with exceptional strength and elasticity. Wrapping silk, or aciniform silk, is the toughest of the spider silks, and has a very different protein composition than other spider silks. Here, we present the characterization of an aciniform protein (AcSp1) subunit named W1, ...

journal_title：FEBS letters

authors： Xu L,Tremblay ML,Orrell KE,Leclerc J,Meng Q,Liu XQ,Rainey JK

更新日期：2013-10-01 00:00:00

abstract：:Apolipoprotein E- (ApoE-) rich high-density lipoprotein (HDL), which was prepared from the bound fraction of normolipemic volunteers on heparin-Sepharose and from a hyperalphalipoproteinemic patient, potently inhibited aggregation of human platelets in a dose-dependent fashion. Dimyristoyl phosphatidylcholine liposome...

journal_title：FEBS letters

authors： Higashihara M,Kinoshita M,Teramoto T,Kume S,Kurokawa K

更新日期：1991-04-22 00:00:00

abstract：:Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syn...

journal_title：FEBS letters

authors： Ying Y,Li L,Cao W,Yan D,Zeng Q,Kong X,Lu L,Yan M,Xu X,Qu J,Su Q,Ma X

更新日期：2012-10-19 00:00:00

abstract：:G-quadruplex structures in the 5' UTR of mRNAs have been shown to suppress translation. However, all experiments published so far made use of artificial reporter systems. Here, we investigate for the first time the biological effects of the genomic disruption of a G-quadruplex by CRISPR/Cas9. In melanoma cells lacking...

journal_title：FEBS letters

authors： Serikawa T,Eberle J,Kurreck J

更新日期：2017-11-01 00:00:00

abstract：:CDK11(p58), a 58kDa protein of the PITSLRE kinase family, plays an important role in cell cycle progression, and is closely related to cell apoptosis. To gain further insight into the function of CDK11(p58), we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. He...

journal_title：FEBS letters

authors： Zong H,Li Z,Liu L,Hong Y,Yun X,Jiang J,Chi Y,Wang H,Shen X,Hu Y,Niu Z,Gu J

更新日期：2005-07-04 00:00:00

abstract：:The protein RPE65 has an important role in retinoid processing and/or retinoid transport in the eye. Retinoids are involved in cell differentiation, embryogenesis and carcinogenesis. Since the kidney is known as an important site for retinoid metabolism, the expression of RPE65 in normal kidney and transformed kidney ...

journal_title：FEBS letters

authors： Ma JX,Zhang D,Laser M,Brownlee NA,Re GG,Hazen-Martin DJ,Redmond TM,Crouch RK

更新日期：1999-06-11 00:00:00

abstract：:The myristoylated N-terminal latching to the C-terminal lobe of c-Abl was recently demonstrated to be an important regulatory element for the kinase, playing a role similar to that of the tyrosine-phosphorylated C-terminal tail of c-Src. A potential mechanism for activating c-Abl is the dissociation of the myristoylat...

journal_title：FEBS letters

authors： Zhou HX

更新日期：2003-09-25 00:00:00

abstract：:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...

journal_title：FEBS letters

authors： Will A,Tanner W

更新日期：1996-02-26 00:00:00

abstract：:Post-absorptive gluconeogenesis from lactate measured in vivo increases 3-fold in 24-month-old rats compared to 3-month-old animals. Fractional lactate turnover rates showed no significant differences between the two groups of animals. Lower plasma glucose concentrations and insulin-glucagon ratios may explain the inc...

journal_title：FEBS letters

authors： Satrústegui J,Cuezva JM,Machado A

更新日期：1986-03-03 00:00:00

abstract：:The pl6INK4a tumor suppressor negatively regulates progression through the G1 phase of the mammalian cell cycle. To mimic the downmodulation of p16INK4a commonly seen in cancer, we designed and characterized a hammerhead ribozyme against exon E1alpha of the murine pl6INK4a transcript. Stable expression of the ribozyme...

journal_title：FEBS letters

authors： Nylandsted J,Rohde M,Bartek J,Strauss M

更新日期：1998-09-25 00:00:00

abstract：:We examined whether a proposed spatial proximity between Asp114(2.50) and Asn332(7.49) affected the functional properties of the mu opioid receptor. The D114(2.50)N mutant had reduced binding affinities for morphine, DAMGO and CTAP, but not for naloxone and [3H]diprenorphine; this mutation also abolished agonist-induc...

journal_title：FEBS letters

authors： Xu W,Ozdener F,Li JG,Chen C,de Riel JK,Weinstein H,Liu-Chen LY

更新日期：1999-03-26 00:00:00

abstract：:A structure of residues 1-177 of the cyclophilin domain of a large divergent cyclophilin from the filarial nematode parasite Brugia malayi has been crystallised and solved in two different crystal forms. The active site has a similar structure to that of human cyclophilin A. Two of the 13 residues important in forming...

journal_title：FEBS letters

authors： Taylor P,Page AP,Kontopidis G,Husi H,Walkinshaw MD

更新日期：1998-03-27 00:00:00