RORalpha1 and RORalpha4 suppress TNF-alpha-induced VCAM-1 and ICAM-1 expression in human endothelial cells.


:Retinoic acid receptor-related orphan receptor-alpha (RORalpha) is a nuclear orphan receptor. Adenovirus-mediated overexpression of RORalpha1 and RORalpha4 suppressed tumor necrosis factor-alpha (TNF-alpha)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells. Overexpression of RORalpha1 and RORalpha4 also suppressed TNF-alpha-stimulated translocation of p50 and p65 to the nucleus. In contrast, dominant-negative deletion mutants of RORalpha1 and RORalpha4 failed to suppress the induction of VCAM-1 and ICAM-1 and translocations of p50 and p65. These results suggest that RORalpha1 and RORalpha4 regulate the inflammatory responses via inhibition of the nuclear factor-kappaB signaling pathway in endothelial cells.




FEBS letters


Migita H,Satozawa N,Lin JH,Morser J,Kawai K





Has Abstract


2004-01-16 00:00:00














  • Modulation of cofactor requirement for the activation of protein kinase C by heparin. Possible effect at the regulatory domain.

    abstract::Heparin was found to stimulate the phosphorylation of histone H1 but not protamine sulfate catalyzed by Ca2+/phospholipid-dependent protein kinase (protein kinase C or PKC). The effect of heparin on histone H1 phosphorylation appeared to be due to an increase in phosphatidylserine affinity for PKC activation in the pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Goueli SA,Hanten JA,Ahmed K

    更新日期:1991-05-06 00:00:00

  • Pyrimidine tract binding protein and La autoantigen interact differently with the 5' untranslated regions of lentiviruses and oncoretrovirus mRNAs.

    abstract::Retrovirus genomic mRNA exhibits a several hundred nucleotides-long untranslated region (5' UTR) which encloses many control elements required for retrovirus replication. In addition, this 5' UTR contains translation regulatory elements, such as internal ribosome entry sites (IRESes) that have been described in oncore...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Waysbort A,Bonnal S,Audigier S,Estève JP,Prats AC

    更新日期:2001-02-09 00:00:00

  • A new mutation in the pufL gene responsible for the terbutryn resistance phenotype in Rubrivivax gelatinosus.

    abstract::Rubrivivax gelatinosus is a facultative phototrophic non-sulfur bacterium belonging to the beta subclass of the purple bacteria. A terbutryn-resistant mutant of R. gelatinosus has been isolated and characterized. Increased resistance levels to terbutryn (300-fold), atrazine (6-fold) and o-phenanthroline (3-fold) were ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ouchane S,Picaud M,Astier C

    更新日期:1995-10-23 00:00:00

  • The endothelial monocyte-activating polypeptide II (EMAP II) is a substrate for caspase-7.

    abstract::Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Behrensdorf HA,van de Craen M,Knies UE,Vandenabeele P,Clauss M

    更新日期:2000-01-21 00:00:00

  • Biochemical characterization and subcellular localization of the sterol C-24(28) reductase, erg4p, from the yeast saccharomyces cerevisiae.

    abstract::The yeast ERG4 gene encodes sterol C-24(28) reductase which catalyzes the final step in the biosynthesis of ergosterol. Deletion of ERG4 resulted in a complete lack of ergosterol and accumulation of the precursor ergosta-5,7,22,24(28)-tetraen-3beta-ol. An erg4 mutant strain exhibited pleiotropic defects such as hypers...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zweytick D,Hrastnik C,Kohlwein SD,Daum G

    更新日期:2000-03-17 00:00:00

  • A novel mechanism of resistance to alpha-difluoromethylornithine induced by cycloheximide. Growth with abnormally low levels of putrescine and spermidine.

    abstract::Treatment of the chemically transformed fibroblasts BP-A31 and other cell lines with low concentrations of cycloheximide (CHM) for 72 h followed by the removal of the protein synthesis inhibitor leads to the proliferation of alpha-difluoromethylornithine (DFMO)-resistant phenotypes. These drug-resistant cells contain ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Medrano EE,Burrone OR,Ferrer MM,Cafferata EG,Algranati ID

    更新日期:1986-09-29 00:00:00

  • Rescue of a neutralizing anti-viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells.

    abstract::The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this rea...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gargano N,Cattaneo A

    更新日期:1997-09-15 00:00:00

  • Sustained activation of AMP-activated protein kinase induces c-Jun N-terminal kinase activation and apoptosis in liver cells.

    abstract::The aim of this work was to study the effect of a sustained activation of AMP-activated protein kinase (AMPK) on liver cell survival. AMPK activation was achieved by incubating FTO2B cells with AICA-riboside, which is transformed into ZMP, an AMP analogue, or by adenoviral transfection of hepatocytes with a constituti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meisse D,Van de Casteele M,Beauloye C,Hainault I,Kefas BA,Rider MH,Foufelle F,Hue L

    更新日期:2002-08-28 00:00:00

  • High concentrations of low density lipoprotein decrease basement membrane-associated heparan sulfate proteoglycan in cultured endothelial cells.

    abstract::The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olgemöller B,Schleicher ED,Schwaabe S,Guretzki HJ,Gerbitz KD

    更新日期:1990-05-07 00:00:00

  • Catechin derivatives: specific inhibitor for caspases-3, 7 and 2, and the prevention of apoptosis at the cell and animal levels.

    abstract::Tea-catechin derivatives are shown to inhibit activities of caspases-3, 2 and 7 in vitro, and prevented experimental apoptosis at the cell and animal levels. Epigallo-catechin-gallate showed the strongest inhibition at 1 x 10(-7)M to these caspases, but cysteine cathepsins and caspase-8 were not inhibited. Caspase-3 i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Katunuma N,Ohashi A,Sano E,Ishimaru N,Hayashi Y,Murata E

    更新日期:2006-02-06 00:00:00

  • Molecular cloning of a novel protein-tyrosine phosphatase SH-PTP3 with sequence similarity to the src-homology region 2.

    abstract::Protein-tyrosine phosphorylation and dephosphorylation are directly associated with cellular growth, signal transduction, and neoplastic transformation. Here we report the isolation of a complementary DNA (cDNA) clone encoding a novel protein-tyrosine phosphatase (PTP) from a human T cell PEER cDNA library. The predic...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Adachi M,Sekiya M,Miyachi T,Matsuno K,Hinoda Y,Imai K,Yachi A

    更新日期:1992-12-21 00:00:00

  • NMR structural studies of the myristoylated N-terminus of ADP ribosylation factor 6 (Arf6).

    abstract::Arf proteins are guanine nucleotide binding proteins that are implicated in endocytotic pathways and vesicle trafficking. The two widely studied isoforms of Arf proteins (Arf1 and Arf6) have different cellular functions and localizations but similar structures. Arf proteins have an N-terminal helix with a covalently b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gizachew D,Oswald R

    更新日期:2006-07-24 00:00:00

  • Convenient plasmid vectors for construction of chimeric mouse/human antibodies.

    abstract::Chimeric antibodies composed of mouse-derived variable regions and human-derived constant regions have been developed for clinical use. However, construction of chimeric mouse/human genes in expression vectors is time-consuming work. In this study, we developed convenient vectors for construction of chimeric mouse/hum...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kameyama K,Imai K,Itoh T,Taniguchi M,Miura K,Kurosawa Y

    更新日期:1989-02-27 00:00:00

  • Identification of a SecM segment required for export-coupled release from elongation arrest.

    abstract::SecM in Escherichia coli has two functionally crucial regions. The arrest motif near the C-terminus interacts with the ribosomal exit tunnel to arrest its own translational elongation. The signal sequence at the N-terminus directs the SecM nascent polypeptide to the Sec-mediated export pathway to release the arrested ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakamori K,Chiba S,Ito K

    更新日期:2014-08-25 00:00:00

  • Modification of the GABA/benzodiazepine receptor with the arginine reagent, 2,3-butanedione.

    abstract::Treatment of either crude or purified preparations of the gamma-aminobutyrate (GABA)/benzodiazepine receptor complex with arginine-specific reagents resulted in a time- and concentration-dependent loss of [3H]muscimol binding activity. Following exposure to either 2,3-butanedione or phenylglyoxal (less than or equal t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Widdows KB,Kirkness EF,Turner AJ

    更新日期:1987-09-28 00:00:00

  • Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance.

    abstract::The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Viglino P,Fogolari F,Formisano S,Bortolotti N,Damante G,Di Lauro R,Esposito G

    更新日期:1993-12-28 00:00:00

  • Augmentation of urea-synthetic capacity by inhibition of nitric oxide synthesis in butyrate-induced differentiated human hepatocytes.

    abstract::We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate tr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoon JH,Lee HS,Kim TH,Woo GH,Kim CY

    更新日期:2000-06-02 00:00:00

  • 15N and 1H NMR evidence for multiple conformations of the complex of dihydrofolate reductase with its substrate, folate.

    abstract::The binding of folate to Lactobacillus casei dihydrofolate reductase in the presence and absence of NADP+ has been studied by 15N NMR, using [5-15N]folate. In the presence of NADP+, three separate signals were observed for the single 15N atom, in agreement with our earlier evidence from 1H and 13C NMR for multiple con...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Birdsall B,De Graw J,Feeney J,Hammond S,Searle MS,Roberts GC,Colwell WT,Crase J

    更新日期:1987-06-08 00:00:00

  • Subcellular localization of the pyoverdine biogenesis machinery of Pseudomonas aeruginosa: a membrane-associated "siderosome".

    abstract::The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Imperi F,Visca P

    更新日期:2013-11-01 00:00:00

  • Rabbit skeletal muscle myosin. Unfolded carboxyl-terminus and its role in molecular assembly.

    abstract::We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Maeda K,Rösch A,Maéda Y,Kalbitzer HR,Wittinghofer A

    更新日期:1991-04-09 00:00:00

  • Mitochondrial ryanodine receptors and other mitochondrial Ca2+ permeable channels.

    abstract::Ca(2+) channels that underlie mitochondrial Ca(2+) transport first reported decades ago have now just recently been precisely characterized electrophysiologically. Numerous data indicate that mitochondrial Ca(2+) uptake via these channels regulates multiple intracellular processes by shaping cytosolic and mitochondria...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Ryu SY,Beutner G,Dirksen RT,Kinnally KW,Sheu SS

    更新日期:2010-05-17 00:00:00

  • Unique molecular properties of superoxide dismutase from teleost fish skin.

    abstract::A unique Cu,Zn-SOD was found and isolated from plaice Paralichthys olivaceus skin. Surprisingly, the properties of purified fish skin SOD were very different from those of SOD from other sources reported so far. The purified SOD was composed of four same subunits of 16 kDa and the molecular weight of the native SOD wa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakano T,Sato M,Takeuchi M

    更新日期:1995-02-27 00:00:00

  • Haemozoin (beta-haematin) biomineralization occurs by self-assembly near the lipid/water interface.

    abstract::Several blood-feeding organisms, including the malaria parasite detoxify haem released from host haemoglobin by conversion to the insoluble crystalline ferriprotoporphyrin IX dimer known as haemozoin. To date the mechanism of haemozoin formation has remained unknown, although lipids or proteins have been suggested to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Egan TJ,Chen JY,de Villiers KA,Mabotha TE,Naidoo KJ,Ncokazi KK,Langford SJ,McNaughton D,Pandiancherri S,Wood BR

    更新日期:2006-09-18 00:00:00

  • Creutzfeldt-Jakob infection increases adenylate cyclase activity in specific regions of guinea pig brain.

    abstract::Creutzfeldt-Jakob disease is a slow, infectious, progressive neurological disorder which results in human dementia. Synaptic membranes from various brain regions of guinea pigs infected with Creutzfeldt-Jakob disease show increased guanyl nucleotide- or 5-hydroxytryptamine-mediated activation of adenylate cyclase. Thi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rasenick MM,Valley S,Manuelidis EE,Manuelidis L

    更新日期:1986-03-17 00:00:00

  • Modulation of O2 reduction in Saccharomyces cerevisiae mitochondria.

    abstract::Respiratory supercomplex factor (Rcf) 1 is a membrane-bound protein that modulates the activity of cytochrome c oxidase (CytcO) in Saccharomyces cerevisiae mitochondria. To investigate this regulatory mechanism, we studied the interactions of CytcO with potassium cyanide (KCN) upon removal of Rcf1. While the addition ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Rydström Lundin C,Brzezinski P

    更新日期:2017-12-01 00:00:00

  • isomiRex: web-based identification of microRNAs, isomiR variations and differential expression using next-generation sequencing datasets.

    abstract::We present an open-access web platform isomiRex, to identify isomiRs and on the fly graphical visualization of the differentially expressed miRNAs in control as well as treated library. The open-access web-platform is not restricted only to NGS sequence dataset from animals and potentially analyzes a wider dataset for...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sablok G,Milev I,Minkov G,Minkov I,Varotto C,Yahubyan G,Baev V

    更新日期:2013-08-19 00:00:00

  • Evidence for multiple interacting binding sites in bovine tryptase.

    abstract::The interaction of bovine pancreatic trypsin inhibitor (BPTI) and bovine tryptase, which are co-localized in the same granules of bovine mast cells, has been analyzed at 30 degrees C in 0.1 M Tris-HCl, pH 8.0. The analysis has unravelled that the functional unit of bovine tryptase is formed of (at least) four binding ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fiorucci L,Erba F,Coletta M,Ascoli F

    更新日期:1995-04-17 00:00:00

  • Serum amyloid A and protein AA: molecular mechanisms of a transmissible amyloidosis.

    abstract::Systemic AA-amyloidosis is a complication of chronic inflammatory diseases and the fibril protein AA derives from the acute phase reactant serum AA. AA-amyloidosis can be induced in mice by an inflammatory challenge. The lag phase before amyloid develops can be dramatically shortened by administration of a small amoun...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Westermark GT,Westermark P

    更新日期:2009-08-20 00:00:00

  • Dynamic analysis of a genomic island in Magnetospirillum sp. strain AMB-1 reveals how magnetosome synthesis developed.

    abstract::The entire structure of a 98 kb genomic region that abounds in genes related to magnetosome synthesis was first described in the Magnetospirillum sp. strain AMB-1. The deletion of this 98 kb genomic region and the circular form after excision from the chromosome was detected by PCR amplification. This strongly suggest...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fukuda Y,Okamura Y,Takeyama H,Matsunaga T

    更新日期:2006-02-06 00:00:00

  • The biosynthetic routes for carbon monoxide and cyanide in the Ni-Fe active site of hydrogenases are different.

    abstract::The incorporation of carbon into the carbon monoxide and cyanide ligands of [NiFe]-hydrogenases has been investigated by using (13)C labelling in infrared studies of the Allochromatium vinosum enzyme and by (14)C labelling experiments with overproduced Hyp proteins from Escherichia coli. The results suggest that the b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roseboom W,Blokesch M,Böck A,Albracht SP

    更新日期:2005-01-17 00:00:00