Structural domains in RNAi.

abstract：:Benzimidazoles are widely used as anthelmintic agents and systemic fungicides. In susceptible organisms, benzimidazoles bind to beta-tubulin and block microtubule polymerization. To further characterize this interaction, site-directed mutagenesis followed by gene replacement was used to change Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Li J,Katiyar SK,Edlind TD

更新日期：1996-04-29 00:00:00

abstract：:We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase. Three different mechanisms of inhibition were identified: at low concentrations (< 100 nM), the cleavage of the RNA template by the retroviral RNase H at the ...

journal_title：FEBS letters

authors： Boiziau C,Moreau S,Toulmé JJ

更新日期：1994-03-07 00:00:00

abstract：:During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....

journal_title：FEBS letters

authors： Gingrich JA,Andersen PH,Tiberi M,el Mestikawy S,Jorgensen PN,Fremeau RT Jr,Caron MG

更新日期：1992-11-09 00:00:00

abstract：:Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but d...

journal_title：FEBS letters

authors： Petit T,Blázquez MA,Gancedo C

更新日期：1996-01-08 00:00:00

abstract：:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1...

journal_title：FEBS letters

authors： Baumruk F,Flachs P,Horáková M,Floryk D,Kopecký J

更新日期：1999-02-12 00:00:00

abstract：:Anion channels/transporters appear as key players in signaling pathways leading to the adaptation of plant cells to abiotic and biotic environmental stresses, in the control of metabolism and in the maintenance of electrochemical gradients. Focusing on the most recent advances, this review aims at providing a descript...

journal_title：FEBS letters

authors： de Angeli A,Thomine S,Frachisse JM,Ephritikhine G,Gambale F,Barbier-Brygoo H

更新日期：2007-05-25 00:00:00

abstract：:This paper reviews our present knowledge of sphingomyelinases as enzymes, and as enzymes acting on a membrane constituent lipid, sphingomyelin. Six types of sphingomyelinases are considered, namely acidic, secretory, Mg(2+)-dependent neutral, Mg(2+)-independent neutral, alkaline, and bacterial enzymes with both phosph...

journal_title：FEBS letters

authors： Goñi FM,Alonso A

更新日期：2002-10-30 00:00:00

abstract：:The retromer complex is a key element of the endosomal protein sorting machinery that is conserved through evolution and has been shown to play a role in diseases such as Alzheimer's disease and Parkinson's disease. Through sorting various membrane proteins (cargo), the function of retromer complex has been linked to ...

journal_title：FEBS letters

authors： Mukadam AS,Seaman MN

更新日期：2015-09-14 00:00:00

abstract：:α-Synuclein (αSyn) is a key player in the pathogenesis of Parkinson's disease and other synucleinopathies. Here, we report the existence of a novel soluble α-helical conformer of αSyn, obtained through transient interaction with lipid interfaces, and propose dynamic oligomerization as the mechanism underlying its stab...

journal_title：FEBS letters

authors： Rovere M,Sanderson JB,Fonseca-Ornelas L,Patel DS,Bartels T

更新日期：2018-05-01 00:00:00

abstract：:One of the main objectives in the analysis of microarray experiments is the identification of genes that are differentially expressed under two experimental conditions. This task is complicated by the noisiness of the data and the large number of genes that are examined simultaneously. Here, we present a novel techniq...

journal_title：FEBS letters

authors： Breitling R,Armengaud P,Amtmann A,Herzyk P

更新日期：2004-08-27 00:00:00

abstract：:Calf chymosin molecules exist in the two alternative structural forms: the first one has S1 and S3 binding pockets occluded by its own Tyr77 residue (the self-inhibited form); the second has these pockets free for a substrate binding (the active form). The preliminary incubation of the enzyme with a pentapeptide corre...

journal_title：FEBS letters

authors： Gustchina E,Rumsh L,Ginodman L,Majer P,Andreeva N

更新日期：1996-01-22 00:00:00

abstract：:We present the three-dimensional structure of the N-terminal FK506-binding protein (FKBP)-like domain of the immunophilin FKBP42 from Arabidopsis thaliana. The data provide the structural background for the explanation of key functional properties reported previously. ...

journal_title：FEBS letters

authors： Weiergräber OH,Eckhoff A,Granzin J

更新日期：2006-01-09 00:00:00

abstract：:The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

journal_title：FEBS letters

authors： Marais RM,Nguyen O,Woodgett JR,Parker PJ

更新日期：1990-12-17 00:00:00

abstract：:The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...

journal_title：FEBS letters

authors： Rosenblum JS,Nomanbhoy TK,Kozarich JW

更新日期：2013-06-27 00:00:00

abstract：:Red blood cells from patients homozygotes for hemoglobin S (HbS) have been studied using a computer-controlled microspectrophotometer, which allows measurements of spectra and dynamics to be undertaken in a single erythrocyte. Complete photodissociation of HbCO results in polymerization of intracellular deoxyhemoglobi...

journal_title：FEBS letters

authors： Coletta M,Alayash AI,Wilson MT,Benedetti PA,Evangelista V,Brunori M

更新日期：1988-08-15 00:00:00

abstract：:The complete amino acid sequence of a mammalian acetylcholinesterase from fetal bovine serum (FBS AChE) is presented. This enzyme has a high degree of sequence identity with other cholinesterases, liver carboxyesterases, esterase-6, lysophospholipase, and thyroglobulin. The locations of 191 amino acids in 10 regions o...

journal_title：FEBS letters

authors： Doctor BP,Chapman TC,Christner CE,Deal CD,De La Hoz DM,Gentry MK,Ogert RA,Rush RS,Smyth KK,Wolfe AD

更新日期：1990-06-18 00:00:00

abstract：:We investigated whether activation of integrin receptors could modulate the expression of monocyte chemotactic protein-1 (MCP-1) in human hepatic stellate cells (HSC), mesenchymal cells responsible for extracellular matrix synthesis within the liver. When compared to non-adherent cells, HSC plated on collagen types I ...

journal_title：FEBS letters

authors： Marra F,Pastacaldi S,Romanelli RG,Pinzani M,Ticali P,Carloni V,Laffi G,Gentilini P

更新日期：1997-09-08 00:00:00

abstract：:We report the identification of bovine miRNAs by cloning small RNAs from adipose tissue and the mammary gland. Fifty-nine distinct miRNAs were identified, five of them were not homologous to known mammalian miRNAs, and many of them had 3' and/or 5' end variants. Ribonuclease protection assays indicated that miR-23a an...

journal_title：FEBS letters

authors： Gu Z,Eleswarapu S,Jiang H

更新日期：2007-03-06 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...

journal_title：FEBS letters

authors： Wang Z,Takemori H,Halder SK,Nonaka Y,Okamoto M

更新日期：1999-06-18 00:00:00

abstract：:A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...

journal_title：FEBS letters

authors： Gafvelin G,Carlquist M,Mutt V

更新日期：1985-05-20 00:00:00

abstract：:The effects of metal cations on the activation of recombinant human SK4 (also known as hIK1 or hKCa4) channels, expressed in HEK 293 cells, were tested using patch clamp recording. Of the nine metals tested, cobalt, iron, magnesium, and zinc did not activate the SK4 channels when applied, at concentrations up to 100 m...

journal_title：FEBS letters

authors： Cao YJ,Houamed KM

更新日期：1999-03-05 00:00:00

abstract：:Low-density lipoprotein receptor-related protein 4 (Lrp4) is essential for pre- and post-synaptic specialization at the neuromuscular junction (NMJ), an indispensable synapse between a motor nerve and skeletal muscle. Muscle-specific receptor tyrosine kinase MuSK must form a complex with Lrp4 to organize postsynaptic ...

journal_title：FEBS letters

authors： Hoshi T,Tezuka T,Yokoyama K,Iemura S,Natsume T,Yamanashi Y

更新日期：2013-11-29 00:00:00

abstract：:The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A...

journal_title：FEBS letters

authors： Wei AZ,Mayr I,Bode W

更新日期：1988-07-18 00:00:00

abstract：:The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

journal_title：FEBS letters

authors： Höcker B,Schmidt S,Sterner R

更新日期：2002-01-16 00:00:00

abstract：:The thermal blockage of QA-. oxidation was analysed in PS II membrane fragments by monitoring flash-induced changes of the relative fluorescence quantum yield as a function of temperature. The results obtained reveal: (a) in dark-adapted samples the fraction of QA-. that is not reoxidised within a time domain of 10 s ...

journal_title：FEBS letters

authors： Reifarth F,Renger G

更新日期：1998-05-29 00:00:00

abstract：:Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 microgram/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (...

journal_title：FEBS letters

authors： Deigner HP,Claus R

更新日期：1996-05-06 00:00:00

abstract：:Using a cationic lipid bilayer, we show that DNA can be reliably adsorbed to the bilayer surface for atomic force microscopy (AFM) in aqueous buffers at high resolution. The measured width of the dsDNA is close to 2 nm, and a periodic modulation on dsDNA is reproducibly detected by the AFM. The measured period is 3.4 ...

journal_title：FEBS letters

authors： Mou J,Czajkowsky DM,Zhang Y,Shao Z

更新日期：1995-09-11 00:00:00

abstract：:DNA complementary to mRNA extracted from the thyroid glands of patients suffering from medullary carcinoma of the thyroid (MCT), a calcitonin-producing tumour, was inserted in the Pst site of pBR 322 by G-C tailing. The recombinant plasmids were used to transform Escherichia coli DP 50. Ampicillin-resistant clones wer...

journal_title：FEBS letters

authors： Le Moullec JM,Jullienne A,Chenais J,Lasmoles F,Guliana JM,Milhaud G,Moukhtar MS

更新日期：1984-02-13 00:00:00

abstract：:Magainins are a family of antimicrobial peptides present in the skin extracts of Xenopus laevis. Both magainin-1 and -2 do not have any significant effect on the activity of protein kinase C (PKC). Magainin-2 was found to be readily phosphorylated by PKC to 0.5 mol 32P/mol of peptide. Neither magainin-1, which has a s...

journal_title：FEBS letters

authors： Nakabayashi H,Brown JH,Morell JL,Chen HC,Huang KP

更新日期：1990-07-02 00:00:00