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Single cell microspectroscopy reveals that erythrocytes containing hemoglobin S retain a 'memory' of previous sickling cycles.

Abstract：

:Red blood cells from patients homozygotes for hemoglobin S (HbS) have been studied using a computer-controlled microspectrophotometer, which allows measurements of spectra and dynamics to be undertaken in a single erythrocyte. Complete photodissociation of HbCO results in polymerization of intracellular deoxyhemoglobin S and deformation of the cell. This is associated with a delayed optical change, which, for the same cell, was found to be highly reproducible between repeated cycles of sickling. Comparison of photographic records and absorbance time courses indicates that an erythrocyte, once having undergone a photochemically induced sickling event, always deforms along the same axis during subsequent cycles. This behaviour implies that the cell retains a 'memory' of its previous cycle(s), possibly via slow relaxations of the membrane. In addition, rebinding of CO to intracellular hemoglobin was found to be slower if measured after deformation of the cell, with possible important implications for the pathological mechanism of sickling.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Coletta M,Alayash AI,Wilson MT,Benedetti PA,Evangelista V,Brunori M

doi

10.1016/0014-5793(88)80299-0

subject

Has Abstract

pub_date

1988-08-15 00:00:00

pages

127-31

issue

1

eissn

0014-5793

issn

1873-3468

pii

0014-5793(88)80299-0

journal_volume

236

pub_type

杂志文章

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