Abstract:
:Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced a segmentally isotope-labeled PQBP1 composed of a non-labeled segment (residues 1-219; N-segment) and a (13)C/(15)N-labeled segment (residues 220-265; C-segment). Our results demonstrate that the segmental isotope-labeling combined with NMR spectroscopy is useful for detecting a very weak intra-molecular interaction in an intrinsically disordered protein.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nabeshima Y,Mizuguchi M,Kajiyama A,Okazawa Hdoi
10.1016/j.febslet.2014.10.028subject
Has Abstractpub_date
2014-12-20 00:00:00pages
4583-9issue
24eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00775-3journal_volume
588pub_type
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