Abstract:
:A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11)beta precursor protein consisting of 499 amino acids including an extension peptide of 24 amino acids at the NH2-terminus. The cDNA contains 1247 nucleotides at the 3'-noncoding region including 51 nucleotides of poly A, but lacks the 5'-noncoding region. The deduced amino acid sequence shows 61% similarity to that of bovine P-450(11)beta. Putative binding sites for heme and steroid are highly conserved among steroidogenic P-450s of known structure.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nonaka Y,Matsukawa N,Morohashi,Omura T,Ogihara T,Teraoka H,Okamoto Mdoi
10.1016/0014-5793(89)81053-1subject
Has Abstractpub_date
1989-09-11 00:00:00pages
21-6issue
1eissn
0014-5793issn
1873-3468journal_volume
255pub_type
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